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1. 发明公开

EP2081070A3 Polariscopic phase microscope 审中-公开
标题翻译： Polariskopisches相差显微镜
公开(公告)号：EP2081070A3
公开(公告)日：2009-07-29
申请号：EP09150365.6
申请日：2009-01-12
申请人： Gwangju Institute of Science and Technology
发明人： Kim, Dug Young , Lee, Ji Yong
IPC分类号： G02B21/14 , G02B21/00
CPC分类号： G02B21/0092 , G02B21/14
摘要： The present invention relates to a polariscopic phase microscope that can precisely observe a specimen, and more specifically, to a polariscopic phase microscope that can observe a structure and change of a physiological cell by using a phase contrast of light passing through components of the physiological cell. There is provided a polariscopic phase microscope according to the present invention comprising: an optical image generator that acquires images for a specimen to be observed; an object plane onto which light beams of the images acquired from the optical image generator are projected; a first transform lens that performs primary Fourier transformation on the light beams passing through the object plane; a λ/4 wavelength plate that is positioned to be spaced by a focal distance of the first transform lens from the first transform lens; a secondary transform lens that performs secondary Fourier transformation on the light beams passing through the λ/4 wavelength plate; and a phase image generator including a photo detector on which the images of the light beams subjected to the secondary Fourier transformation is focused.
The present invention has an effect that can observe the structure and motion of the physiological cell by acquiring the quantitative phase information of the biological specimen such as the physiological cell.

2. 发明公开

EP2096430A2 Apparatus and method for obtaining images using coherent anti-stokes Raman scattering 审中-公开
标题翻译：装置和使用相干反斯托克斯拉曼散射成像方法
公开(公告)号：EP2096430A2
公开(公告)日：2009-09-02
申请号：EP09153244.0
申请日：2009-02-19
申请人： Gwangju Institute of Science and Technology
发明人： Kim, Dug Young , Cho, Seung Bum
IPC分类号： G01N21/65
CPC分类号： G01N21/65 , G01J3/44 , G01N2021/653
摘要： Disclosed is an apparatus and method for obtaining images using coherent anti-stokes Raman scattering. The apparatus for obtaining images using coherent anti-stokes Raman scattering according to the present invention comprises: a pump light source (401) and a stokes light source (402) that irradiate pump light and stokes light on a sample (S) to generate anti-stokes light having anti-stokes frequency; a reference light source (410) that generates reference light; and an image obtaining unit (430) that obtains the images of the sample using a change in phase of the reference light due to a change in the refractive index of the sample in the vicinity of the anti-stokes frequency. Thereby, the present invention provides an apparatus for obtaining images using coherent anti-stokes Raman scattering that is not affected by non-resonant background signals, that has strong resistance against noise even for weak signals, and that has excellent sensitivity and resolution.
摘要翻译：公开了一种用于使用相干反斯托克斯拉曼散射获得图像的装置和方法。对于使用相干反斯托克斯拉曼获取图像散射雅丁到本发明的设备包括：一个泵浦光源（401）和一个斯托克斯光源（402）那样照射到泵浦光，并在样品（S），以产生反斯托克斯光 -stokes具有光反斯托克斯频; （410）做了一个参考光源生成参考光; 以及图像获得单元（430）没有获得用参照光的相位的变化的试样的图像由于在反斯托克斯频率附近的样本的折射率的改变。由此，本发明提供的装置用于使用相干反斯托克斯拉曼散射并不会受到非共振背景信号获得的图像，并具有针对即使对于弱信号噪声抗性强，并做具有优良的灵敏度和分辨率。

3. 发明公开

EP2096430A3 Apparatus and method for obtaining images using coherent anti-stokes Raman scattering 审中-公开
标题翻译：装置和使用相干反斯托克斯拉曼散射成像方法
公开(公告)号：EP2096430A3
公开(公告)日：2013-02-20
申请号：EP09153244.0
申请日：2009-02-19
申请人： Gwangju Institute of Science and Technology
发明人： Kim, Dug Young , Cho, Seung Bum
IPC分类号： G01N21/65 , G01J3/44
CPC分类号： G01N21/65 , G01J3/44 , G01N2021/653
摘要： Disclosed is an apparatus and method for obtaining images using coherent anti-stokes Raman scattering. The apparatus for obtaining images using coherent anti-stokes Raman scattering according to the present invention comprises: a pump light source (401) and a stokes light source (402) that irradiate pump light and stokes light on a sample (S) to generate anti-stokes light having anti-stokes frequency; a reference light source (410) that generates reference light; and an image obtaining unit (430) that obtains the images of the sample using a change in phase of the reference light due to a change in the refractive index of the sample in the vicinity of the anti-stokes frequency. Thereby, the present invention provides an apparatus for obtaining images using coherent anti-stokes Raman scattering that is not affected by non-resonant background signals, that has strong resistance against noise even for weak signals, and that has excellent sensitivity and resolution.

4. 发明公开

EP2081070A2 Polariscopic phase microscope 审中-公开
标题翻译： Polariskopisches Phasenkontrastmikroskop
公开(公告)号：EP2081070A2
公开(公告)日：2009-07-22
申请号：EP09150365.6
申请日：2009-01-12
申请人： Gwangju Institute of Science and Technology
发明人： Kim, Dug Young , Lee, Ji Yong
IPC分类号： G02B21/14 , G02B21/00
CPC分类号： G02B21/0092 , G02B21/14
摘要： The present invention relates to a polariscopic phase microscope that can precisely observe a specimen, and more specifically, to a polariscopic phase microscope that can observe a structure and change of a physiological cell by using a phase contrast of light passing through components of the physiological cell. There is provided a polariscopic phase microscope according to the present invention comprising: an optical image generator that acquires images for a specimen to be observed; an object plane onto which light beams of the images acquired from the optical image generator are projected; a first transform lens that performs primary Fourier transformation on the light beams passing through the object plane; a λ/4 wavelength plate that is positioned to be spaced by a focal distance of the first transform lens from the first transform lens; a secondary transform lens that performs secondary Fourier transformation on the light beams passing through the λ/4 wavelength plate; and a phase image generator including a photo detector on which the images of the light beams subjected to the secondary Fourier transformation is focused.
The present invention has an effect that can observe the structure and motion of the physiological cell by acquiring the quantitative phase information of the biological specimen such as the physiological cell.
摘要翻译：本发明涉及一种可以精确观察样本的偏光相位显微镜，更具体地说，涉及通过使用通过生理细胞的成分的光的相位对比来观察生理细胞的结构和变化的偏光相显微镜。提供了根据本发明的偏振相显微镜，包括：光学图像生成器，其获取待观察的样本的图像; 投影从光学图像生成器获取的图像的光束的物平面; 对通过物平面的光束进行主傅立叶变换的第一变换透镜; 定位成与第一变换透镜的焦距相隔离的第一变换透镜的»/ 4波长板; 对通过»/ 4波长板的光束进行二次傅立叶变换的次级变换透镜; 以及相关图像发生器，其包括光电检测器，其上经受二级傅里叶变换的光束的图像被聚焦。本发明具有通过获取诸如生理细胞的生物样本的定量相位信息来观察生理细胞的结构和运动的效果。

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