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1. 发明授权

EP2350660B1 IMMOBILIZATION SUBSTRATE AND METHOD FOR PRODUCING THE SAME 有权
标题翻译：固定基片和生产该基片的方法
公开(公告)号：EP2350660B1
公开(公告)日：2016-03-09
申请号：EP09822111.2
申请日：2009-10-23
申请人： FUJIFILM Corporation , The University of Tokyo
发明人： MINAMI, Koichi , TSUZUKI, Hirohiko , UEDA, Hiroshi , IHARA, Masaki
IPC分类号： C07K17/14 , G01N33/543 , G01N33/68
CPC分类号： C07K16/40 , C07K16/18 , C07K2317/56 , Y10T428/269

2. 发明授权

EP2359138B1 IMMOBILIZATION SUBSTRATE AND METHOD FOR PRODUCING THE SAME 有权
标题翻译：固定化及其制造方法
公开(公告)号：EP2359138B1
公开(公告)日：2016-05-04
申请号：EP09830496.7
申请日：2009-12-04
申请人： FUJIFILM Corporation , The University of Tokyo
发明人： MINAMI, Koichi , TSUZUKI, Hirohiko , UEDA, Hiroshi , IHARA, Masaki
IPC分类号： G01N33/543 , C07K19/00 , G01N33/547
CPC分类号： G01N33/54353

3. 发明公开

EP2350660A1 IMMOBILIZATION SUBSTRATE AND METHOD FOR PRODUCING THE SAME 有权
标题翻译：固定基片和生产该基片的方法
公开(公告)号：EP2350660A1
公开(公告)日：2011-08-03
申请号：EP09822111.2
申请日：2009-10-23
申请人： FUJIFILM Corporation , The University of Tokyo
发明人： MINAMI, Koichi , TSUZUKI, Hirohiko , UEDA, Hiroshi , IHARA, Masaki
IPC分类号： G01N33/543 , C07K17/00 , C12N15/09
CPC分类号： C07K16/40 , C07K16/18 , C07K2317/56 , Y10T428/269
摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein the antibody fragments of each set includes at least two types of separate antibody fragments that are capable of recognizing one type of antigen and that are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.
摘要翻译：抗体片段固定化底物包括底物和至少一组抗体片段，其中每组抗体片段包括至少两种类型的分开的抗体片段，其能够识别一种类型的抗原并且独立地固定在以允许一组中的每种抗体片段与相同抗原结合的位置关系处理底物。

4. 发明公开

EP2359138A1 IMMOBILIZATION SUBSTRATE AND METHOD FOR PRODUCING THE SAME 有权
标题翻译：固定化及其制造方法
公开(公告)号：EP2359138A1
公开(公告)日：2011-08-24
申请号：EP09830496.7
申请日：2009-12-04
申请人： FUJIFILM Corporation , The University of Tokyo
发明人： MINAMI, Koichi , TSUZUKI, Hirohiko , UEDA, Hiroshi , IHARA, Masaki
IPC分类号： G01N33/543 , C07K19/00 , G01N33/547
CPC分类号： G01N33/54353
摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein antibody fragments of each set include at least two types of separate antibody fragments, the at least two types of separate antibody fragments include at least one type of labeled antibody fragment having a labeled site labeled with a luminescent substance and at least one type of acceptor antibody fragment having an acceptor site for accepting emission from the labeled antibody fragment, and the at least one type of labeled antibody fragment and the at least one type of acceptor antibody fragment are capable of cooperatively recognizing one type of antigen in combination and are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

5. 发明公开

EP1536005A1 METHOD OF ASSAYING INTERACTION BETWEEN PROTEINS 审中-公开
标题翻译： VERFAHREN ZUM TESTEN DER WECHSELWIRKUNG ZWISCHEN PROTEINEN
公开(公告)号：EP1536005A1
公开(公告)日：2005-06-01
申请号：EP03788129.9
申请日：2003-08-15
申请人： The University of Tokyo
发明人： UEDA, Hiroshi , NAGAMUNE, Teruyuki
IPC分类号： C12N15/09 , C12N15/70 , C12Q1/02 , G01N33/53
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12N15/70
摘要： A novel method for determining interaction between VH fragment and VL fragment of the variable region of an antibody is provided by the present invention. The method according to this invention can be widely used for detection of protein interactions. If a phagemid vector containing an amber codon is used for transformation of an amber suppressor strain of E.coli according to the method of the invention to produce phages, both of the VH fragment and the VL fragment will be displayed on the phage particles. In contrast, if the same vector is used to transformation of an amber non-suppressing strain of E.coli to produce phages, for the presence of the amber codon, only the VH fragment will be displayed on the phage particles, while the VL fragment will be secreted into the culture medium by the E.coli. Thus, display switch occurs. The interaction between the VH fragment and the VL fragment can be determined, by immobilizing the VL fragment secreted into the culture medium on a solid phase and quantifying binding between the VH fragment displayed by phages and the VL fragment.
摘要翻译：本发明提供了用于确定抗体可变区的VH片段和VL片段之间相互作用的新方法。根据本发明的方法可广泛用于蛋白质相互作用的检测。如果含有琥珀密码子的噬菌粒载体用于根据本发明的方法转化大肠杆菌的琥珀抑制菌株以产生噬菌体，那么VH片段和VL片段都将显示在噬菌体颗粒上。相比之下，如果使用相同的载体转化大肠杆菌的琥珀非抑制菌株以产生噬菌体，则对于琥珀密码子的存在，只有VH片段将显示在噬菌体颗粒上，而VL片段将被大肠杆菌分泌到培养基中。因此，显示切换发生。可以通过将分泌到培养基中的VL片段固定在固相上并定量由噬菌体展示的VH片段与VL片段之间的结合来确定VH片段和VL片段之间的相互作用。

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