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1. 发明公开

EP0190893A3 Test kit and method for the determination of streptococcus mutans 失效
标题翻译：测试工具包和方法，用于确定毛霉菌
公开(公告)号：EP0190893A3
公开(公告)日：1987-09-16
申请号：EP86300669
申请日：1986-01-31
申请人： FORSYTH DENTAL INFIRMARY FOR CHILDREN
发明人： Jordan, Harold V. , Marmel, Max
IPC分类号： C12Q01/14
CPC分类号： C12Q1/14 , G01N2333/315 , Y10S435/80 , Y10S435/801 , Y10S435/885
摘要： The semi-quantitive detection of Streptococcus mutans from the oral cavity of a patient in vitro involves introducing saliva from a patient into a diluent solution to which has been added just before the saliva a predetermined powdered amount of bacitracin, and contacting the diluent solution with a sterile medium-coated paddle, then incubating the medium-coated paddle containing the diluent and test saliva, and thereafter semi-quantitively determining the amount of Streptococcus mutans in the test saliva by comparing the percent of colony density on the paddle with a standard Streptococcus mutans colony density chart. The amount of Steptococcus mutans in the oral sample is indicative of the patient's predispostion towards dental caries. A test kit for the determination of Streptococcus mutans includes an asceptic first vial (12) having a paddle (20) bearing a coating of medium (22) comprising agar and sucrose, a second asceptic vial (14) containing a buffered saline solution, a predetermined amount of bacitracin (28) e.g. in pellet form, a carbon dioxide generating composition (26), and standard Streptococcus mutans colony density charts (30).

2. 发明公开

EP0190893A2 Test kit and method for the determination of streptococcus mutans 失效
标题翻译： Reagenzsatz und Verfahren zur Bestimmung von变形链球菌。
公开(公告)号：EP0190893A2
公开(公告)日：1986-08-13
申请号：EP86300669.8
申请日：1986-01-31
申请人： FORSYTH DENTAL INFIRMARY FOR CHILDREN
发明人： Jordan, Harold V. , Marmel, Max
IPC分类号： C12Q1/14
CPC分类号： C12Q1/14 , G01N2333/315 , Y10S435/80 , Y10S435/801 , Y10S435/885
摘要： The semi-quantitive detection of Streptococcus mutans from the oral cavity of a patient in vitro involves introducing saliva from a patient into a diluent solution to which has been added just before the saliva a predetermined powdered amount of bacitracin, and contacting the diluent solution with a sterile medium-coated paddle, then incubating the medium-coated paddle containing the diluent and test saliva, and thereafter semi-quantitively determining the amount of Streptococcus mutans in the test saliva by comparing the percent of colony density on the paddle with a standard Streptococcus mutans colony density chart. The amount of Steptococcus mutans in the oral sample is indicative of the patient's predispostion towards dental caries.
A test kit for the determination of Streptococcus mutans includes an asceptic first vial (12) having a paddle (20) bearing a coating of medium (22) comprising agar and sucrose, a second asceptic vial (14) containing a buffered saline solution, a predetermined amount of bacitracin (28) e.g. in pellet form, a carbon dioxide generating composition (26), and standard Streptococcus mutans colony density charts (30).
摘要翻译：来自患者体外口腔的变形链球菌的半定量检测包括将患者的唾液引入稀释液溶液中，在唾液中加入预定粉末量的杆菌肽，并将稀释液与然后将包含稀释剂和测试唾液的培养基涂覆的培养皿孵育，然后通过将桨上的菌落密度与标准变形链球菌的百分比进行半定量测定来测定测试唾液中变形链球菌的量菌落密度图。口腔样品中变形链球菌的量表明患者对龋齿的预测。用于测定变形链球菌的测试试剂盒包括具有桨叶（20）的无菌第一小瓶（12），所述桨叶（20）承载包含琼脂和蔗糖的培养基（22），第二吸收小瓶（14）缓冲盐水溶液，预定量的杆菌肽（28），例如颗粒形式，二氧化碳生成组合物（26）和标准的变形链球菌集落密度图（30）。

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