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    • 1. 发明公开
    • MULTIPLEXED ANALYSIS OF TARGET NUCLEIC ACIDS
    • MULTIPLEX-ANALYZE VONZIELNUKLEINSÄUREN
    • EP2989215A4
    • 2016-12-28
    • EP14788247
    • 2014-04-25
    • FIREFLY BIOWORKS INC
    • PREGIBON DANIEL CSTONER ISAACFUSCO ANTHONYTACKETT MICHAEL R
    • C12Q1/68C12Q1/25
    • C12Q1/6806C12Q1/682C12Q2525/155C12Q2525/191C12Q2525/207C12Q2563/149C12Q2563/179
    • The present invention provides, among other things, methods of detecting target nucleic acid, comprising steps of: a) contacting a sample with one or more capturing probes, each comprising at least one target capturing sequence, under conditions that permit the one or more capturing probes to capture one or more target nucleic acids in the sample; b) amplifying the captured one or more target nucleic acids in a reaction mixture comprising a detectable entity such that the amplified one or more target nucleic acids are labeled with the detectable entity; c) incubating amplification product with a plurality of re-capturing probes such that the amplified one or more target nucleic acids are re-captured by the plurality of the re-capturing probes; and d) detecting signal generated by detectable entity associated with the re-captured amplified one or more target nucleic acids, wherein the presence and/or abundance of the detectable signal indicates the presence and/or abundance of the one or more target nucleic acids in the sample.
    • 本发明尤其提供了检测靶核酸的方法,包括以下步骤:a)在允许一个或多个捕获的条件下,将样品与一个或多个捕获探针接触,每个捕获探针包含至少一个靶捕获序列 探针以捕获样品中的一种或多种靶核酸; b)在包含可检测实体的反应混合物中扩增捕获的一种或多种靶核酸,使得扩增的一个或多个靶核酸用可检测实体标记; c)用多个再捕获探针孵育扩增产物,使得扩增的一个或多个靶核酸被多个重新捕获探针重新捕获; 和d)检测由与被再捕获的扩增的一个或多个靶核酸相关联的可检测实体产生的信号,其中可检测信号的存在和/或丰度表示一个或多个靶核酸的存在和/或丰度 例子。