专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP0922759B1 Aldehyde dehydrogenase 有权
标题翻译：乙醛脱氢酶
公开(公告)号：EP0922759B1
公开(公告)日：2004-01-28
申请号：EP98122223.5
申请日：1998-11-23
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Miyazaki, Taro , Sugisawa, Teruhide
IPC分类号： C12N9/02 , C12P7/60
CPC分类号： C12N9/0008 , C12P7/60

2. 发明公开

EP0922759A3 Aldehyde dehydrogenase 有权
标题翻译：乙醛脱氢酶
公开(公告)号：EP0922759A3
公开(公告)日：2000-01-19
申请号：EP98122223.5
申请日：1998-11-23
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Miyazaki, Taro , Sugisawa, Teruhide
IPC分类号： C12N9/02 , C12P7/60
CPC分类号： C12N9/0008 , C12P7/60
摘要： A new aldehyde dehydrogenase having the physico-chemical properties:-molecular weight: 150,000 ± 6,000 or 230,000 ± 9,000; substrate specificity:active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c ; optimum pH: 7.0-8.5; and inhibitors: Co 2+ , Cu 2+ , Fe 2+ , Ni 2+ , Zn 2+ , monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter . Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. 2-Keto-L-gulonic acid (2-KGA) can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) a Gluconobacter microorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-KGA from the reaction mixture.

3. 发明公开

EP0790301A2 Aldehyde Dehydrogenase 失效
标题翻译：醛脱氢酶
公开(公告)号：EP0790301A2
公开(公告)日：1997-08-20
申请号：EP97102079.7
申请日：1997-02-10
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Sugisawa, Teruhide
IPC分类号： C12N9/02 , C12P7/60 , C12N1/20
CPC分类号： C12N9/0008 , C12P7/60 , Y10S435/822
摘要： A new aldehyde dehydrogenase having the physico-chemical properties:- molecular weight: 91,000 ± 5,000; substrate specificity:active on aldehyde compounds; inhibition: by Cu 2+ , Zn 2+ , Ni 2+ and EDTA; optimum pH: 6.0-8.5; optimum temperature: 20-40°C; and stimulator: Ca 2+ and PQQ, is derived from a microorganism belonging to the genus Gluconobacter . Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. 2-Keto-L-gulonic acid (2-KGA) can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) a Gluconobacter microorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-KGA from the reaction mixture.
摘要翻译：具有物理化学性质的新型醛脱氢酶： - 分子量：91,000 +/- 5,000; 底物特异性：对醛化合物有活性; 通过Cu 2+，Zn 2+，Ni 2+和EDTA抑制：最适pH：6.0-8.5; 最适温度：20-40℃; 和刺激剂Ca 2+和PQQ来源于属于葡糖杆菌属的微生物。所述醛脱氢酶可以通过在有氧条件下在水性营养培养基中培养能够产生具有上述性质的醛脱氢酶的葡糖杆菌属微生物，破坏微生物细胞并从其中分离和纯化醛脱氢酶微生物破坏细胞的无细胞提取物。 2-酮-L-古洛糖酸（2-KGA）可以由L-山梨糖酮通过在电子受体的存在下将L-山梨醇与（i）醛脱氢酶接触，（ii）能产生醛脱氢酶在有氧条件下在水性介质中或（iii）所述微生物的无细胞提取物，并且在每种情况下从反应混合物中分离得到的2-KGA。

4. 发明公开

EP0728840A3 D-Sorbitol dehydrogenase 失效
标题翻译： D-山梨醇脱氢酶
公开(公告)号：EP0728840A3
公开(公告)日：2001-12-19
申请号：EP96102405.6
申请日：1996-02-17
申请人： F. Hoffmann-La Roche AG
发明人： Hoshino, Tatsuo , Ojima, Setsuko , Sugisawa, Teruhide
IPC分类号： C12N9/04 , C12P19/02
CPC分类号： C12N9/0006 , Y10S435/823
摘要： D-sorbitol dehydrogenase in purified form, which catalyzes the oxidation of D-sorbitol to L-sorbose, has a molecular structure consisting of homologous subunit of molecular weight 79,000±5,000, a substrate specificity for polyols and an optimum pH of 6.0-7.0. Said D-sorbitol dehydrogenase is producible by a process comprising cultivating a microorganism belonging to the genus Gluconobacter or Acetobacter, or a mutant or variant thereof, which is capable of producing the D-sorbitol dehydrogenase in the cells and isolating it from the cells, e.g. by disrupting the cells and isolation from cell-free extract of the disrupted cells. The so-isolated D-sorbitol hydrogenase is useful for catalyzing the oxidation of D-sorbitol to L-sorbose, the latter being an important intermediate for the production of vitamin C.

5. 发明授权

EP0476442B1 L-gulono-gamma-lactone dehydrogenase 失效转让
标题翻译： L-Gulono-Gamma-Lacton脱氢酶
公开(公告)号：EP0476442B1
公开(公告)日：1999-06-02
申请号：EP91114996.1
申请日：1991-09-05
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Matzinger, Peter Karl , Ojima, Setsuko , Sugisawa, Teruhide
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12P17/04

6. 发明公开

EP0476442A2 L-gulono-gamma-lactone dehydrogenase 失效转让
标题翻译： L-Gulono-Gamma-Lacton脱氢酶。
公开(公告)号：EP0476442A2
公开(公告)日：1992-03-25
申请号：EP91114996.1
申请日：1991-09-05
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Matzinger, Peter Karl , Ojima, Setsuko , Sugisawa, Teruhide
IPC分类号： C12N9/04 , C12P17/04
CPC分类号： C12N9/0006 , C12P17/04
摘要： The present invention concerns a novel process for the microbiological manufacture of L-ascorbic acid from L-gulono-gamma-lactone, a process for preparing the enzyme being responsible for the microbiological oxidation of L-gulono-gamma-lactone, and said enzyme in purified form, which is a L-gulono-gamma-lactone dehydrogenase.
摘要翻译：本发明涉及一种用于从L-gulono-γ-内酯微生物制造L-抗坏血酸的新方法，该方法用于制备负责L-gulono-γ-内酯的微生物氧化的酶，所述酶在纯化形式，其是L-古洛宁-γ-内酯脱氢酶。

7. 发明授权

EP0213591B1 Process for the manufacture of keto gulonic acid 失效转让
标题翻译： KETO GULONIC ACID的制造工艺
公开(公告)号：EP0213591B1
公开(公告)日：1992-03-25
申请号：EP86111793.5
申请日：1986-08-26
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Fujiwara, Akiko , Sugisawa, Teruhide , Shinjoh, Masako Dai 3 Ohfuna Park Town G-612 , Setoguchi, Yutaka , Hoshino, Tatsuo
IPC分类号： C12P7/60 , C07D307/62 , C12N1/20
CPC分类号： C12N9/0006 , C12N15/03 , C12P7/60

8. 发明公开

EP0248400A2 Enzym und Verfahren zu seiner Herstellung 失效
标题翻译： Enzym und Verfahren zu seiner Herstellung。
公开(公告)号：EP0248400A2
公开(公告)日：1987-12-09
申请号：EP87107960.4
申请日：1987-06-02
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Fujiwara, Akiko , Hoshino, Tatsuo , Sugisawa, Teruhide
IPC分类号： C12N9/04 , C12P19/02
CPC分类号： C12N9/0006 , C12P19/02 , Y10S435/822
摘要： Die erfindungsgemässe neue L-Sorbose-Dehydrogenase katalysiert die Oxidation von L-Sorbose zu L-Sorboson, dem Vorläufer der 2-Keto-L-gulonsäure, die ihrerseits ein wichtiges Zwischenprodukt bei der Herstellung von Vitamin C ist.
Die erfindungsgemässe Herstellung der neuen L-Sorbose-Dehydrogenase erfolgt durch Kultivieren eines zur Gattung Gluconobacter gehörenden Mikroorganismus oder einer Mutante davon, die zur Herstellung der neuen L-Sorbose-Dehydrogenase in Zellmaterial befähigt sind, Zertrümmern des Zellmaterials, Isolieren der L-Sorbose-Dehydrogenase aus zellfreiem Extrakt des zertrümmerten Zellmaterials, vorzugsweise aus der Membranfraktion des Zellmaterials, sowie Reinigen der L-Sorbose-Dehydrogenase.
摘要翻译：根据本发明的新型L-山梨糖 - 脱氢酶催化L-山梨糖氧化为L-山梨糖醇，其是2-酮-L-古洛糖酸的前体，其又是制备维生素C的重要中间体。 ...根据本发明制备新型L-山梨糖脱氢酶的方法通过培养属于葡糖杆菌属的微生物或该微生物的突变体进行，其能够产生新的L - 脱水山梨醇 - 脱氢酶，分解细胞材料，从破碎细胞材料的无细胞提取物中分离L-山梨糖 - 脱氢酶，优选从细胞材料的膜部分中纯化L-山梨糖 - 脱氢酶，山梨糖脱氢酶。

9. 发明公开

EP0911415A3 Microbiological manufacture of L-ascorbic acid 失效
标题翻译：微生物制造L-抗坏血酸
公开(公告)号：EP0911415A3
公开(公告)日：1999-05-06
申请号：EP98121547.8
申请日：1991-09-05
申请人： F. Hoffmann-La Roche AG
发明人： Hoshino, Tatsuo , Matzinger, Peter Karl , Ojima, Setsuko , Sugisawa, Teruhide
IPC分类号： C12P17/04
CPC分类号： C12N9/0006 , C12P17/04
摘要： The present invention concerns a novel process for the microbiological manufacture of L-ascorbic acid from L-gulono-gamma-lactone.
摘要翻译：本发明涉及由L-古洛糖酸-γ-内酯微生物制造L-抗坏血酸的新方法。

10. 发明公开

EP0366922A1 Fermentation process for producing 2-keto-L-gulonic acid 失效
标题翻译： Fermentationsverfahren zur Herstellung von 2-Keto-L-Gulonsäure。
公开(公告)号：EP0366922A1
公开(公告)日：1990-05-09
申请号：EP89117499.7
申请日：1989-09-21
申请人： F. HOFFMANN-LA ROCHE AG
发明人： Hoshino, Tatsuo , Nomura, Setsuko , Sugisawa, Teruhide
IPC分类号： C12P7/60 , C12P39/00
CPC分类号： C12P7/60 , C12P39/00 , Y10S435/822 , Y10S435/911 , Y10S435/921 , Y10S435/924 , Y10S435/93 , Y10S435/941 , Y10S435/942 , Y10S435/944
摘要： A process of producing 2-keto-L-gulonic acid by fermentative conversion of L,-sorbose utilizing a fermentation system composed of component produced from a microorganism having the identifying characteristics of strain DSM No. 4025 and a yeast component.
摘要翻译：利用由具有菌株DSM No.4025的鉴定特征的微生物产生的组分和酵母成分组成的发酵系统，通过发酵转化L， - 山梨糖来生产2-酮-L-古洛糖酸的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式