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3. 发明公开

EP3173478A1 BIOTECHNOLOGICAL PRODUCTION OF OMEGA-FUNCTIONALISED CARBOXYLIC ACIDS AND ESTERS THEREOF 审中-公开
标题翻译：生物技术研究所大卫·冯·奥马焦恩·芬克斯
公开(公告)号：EP3173478A1
公开(公告)日：2017-05-31
申请号：EP16199533.7
申请日：2016-11-18
申请人： Evonik Degussa GmbH
发明人： Schaffer, Steffen , Haas, Thomas , Brügging, Wilhelm , Meier, Ralf
IPC分类号： C12N9/04 , C12N9/02 , C12N9/10 , C12P7/40 , C12P7/62
CPC分类号： C12P7/62 , C12N9/0006 , C12N9/0077 , C12N9/1029 , C12N9/1096 , C12N15/52 , C12P7/6436 , C12P7/649 , C12Y101/01001 , C12Y101/01002 , C12Y101/0302 , C12Y114/15003 , C12Y203/01075 , C12Y203/01084 , C12Y206/01
摘要： There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of
(i) Enzyme E 1 capable of converting the alkane to the corresponding 1-alkanol;
(ii) Enzyme E 2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal;
(iii) Enzyme E 3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid;
(iv) Enzyme E 4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and
(iv) Enzyme E 5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester,
and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E 20 - E 24 selected from the group consisting of:
- E 20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.22,
- E 21 Acyl-CoA thioesterase, of EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22,
- E 22 Acyl-CoA:ACP transacylase,
- E 23 Polyketide synthase, and
- E 24 Hexanoic acid synthase
摘要翻译：提供了一种用于从至少一种烷烃生产至少一种ω官能化羧酸酯的微生物细胞，其中所述细胞被遗传修饰以增加相对于野生型细胞的表达，所述（i）能够转化烷烃至相应的1-链烷醇; （ii）能够将（i）的1-链烷醇转化为相应的1-链烷醛的酶E 2; （iii）能够将（ⅱ）的1-链烷酸转化成相应的链烷酸的酶E 3; （iv）能够将（iii）的链烷酸转化成相应的链烷酸酯的酶E 4; 和（iv）能够将（iv）的链烷酸酯转化成相应的ω-羟基 - 链烷酸酯的酶E 5，并且其中所述细胞不包含增加相对于野生型细胞的表达的遗传修饰 EC 3.1中的至少一种选自以下的酶E 20 -E 24：EC 3.1.2.14或EC 3.1.2.22的E 20酰基-ACP硫酯酶，-E 21酰基-CoA硫酯酶。 2.2，EC 3.1.2.18，EC 3.1.2.19，EC 3.1.2.20或EC 3.1.2.22，E 22酰基-CoA：ACP转酰基酶，-E 23聚酮化合物合酶和-E 24己酸合酶

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