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1. 发明公开

EP0731951A4 OPTICAL SPECIMEN ANALYSIS SYSTEM AND METHOD 有权
标题翻译：优化探头分析仪器在VERFAHREN
公开(公告)号：EP0731951A4
公开(公告)日：1997-09-17
申请号：EP94909763
申请日：1994-02-24
申请人： E Y LAB INC
发明人： CHU ALBERT E , TAO LIAN
IPC分类号： G01N21/82 , G01N15/14 , G01N21/27 , G01N21/59 , G01N21/86 , G06T7/40 , G06K9/00
CPC分类号： G01N21/8483 , G01N15/1468 , G01N15/1475 , G01N2021/177 , G01N2021/5919 , G01N2021/5923 , G01N2021/593 , G01N2021/5934 , G01N2021/8488 , G06T7/90 , G06T2207/10024 , G06T2207/30004
摘要： A method and system for automatically analyzing the presence of an analyte in a liquid specimen of biological origin are described. The liquid specimen is added to a testing substrate that has a receptor immobilized on a portion of its surface. If analyte is present in the specimen, it will specifically bind to the receptor. A labeled reagent is added to the testing substrate which will bind to the analyte, if present, and generate a color on the testing substrate. The testing substrate is then illuminated and, using electronic equipment, a digital image of its surface is acquired and automatically scanned to locate an area that has the highest color density, which corresponds to the presence of labeled reagent. A measurement of color density, corresponding to pixels per unit area, is generated. An area peripheral to the area of highest color density, which represents background density, is also located and a measured. The presence or absence of analyte in the liquid sample is determined by adjusting the measurement of labeled reagent with the background density measurement in accordance with a predefined mathematical function. In some cases, the result of the test will be interpreted as a binary, positive/negative result according to whether the measurement taken is above or below a given threshold. In other cases, a continuous range of values may be generated from different samples that correspond to the concentration of the analyte present in each tested sample.
摘要翻译：描述了用于自动分析生物来源的液体样品中分析物的存在的方法和系统。将液体样品加入到具有固定在其表面的一部分上的受体的测试基底上。如果分析物存在于样品中，则其将特异性结合受体。将标记的试剂加入到测试底物中，其将结合分析物（如果存在）并在测试底物上产生颜色。然后照射测试基板，并且使用电子设备获取其表面的数字图像并自动扫描以定位具有最高着色密度的区域，其对应于标记试剂的存在。产生对应于每单位面积的像素的颜色浓度的测量。位于最高颜色密度区域外围的区域也代表背景密度，也被测量。通过根据预定义的数学函数调整具有背景密度测量的标记试剂的测量来确定液体样品中分析物的存在或不存在。在某些情况下，根据测量值是否高于或低于给定阈值，测试结果将被解释为二进制正/负结果。在其他情况下，可以从对应于每个测试样品中存在的分析物的浓度的不同样品产生连续范围的值。

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