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2. 发明公开

EP2601304A1 MASS SPECTROMETRIC DIAGNOSIS OF SEPSIS WITHOUT BLOOD CULTURE 有权转让
标题翻译：败血症的无血群众性文化诊断
公开(公告)号：EP2601304A1
公开(公告)日：2013-06-12
申请号：EP11749128.2
申请日：2011-07-29
申请人： Bruker Daltonik GmbH
发明人： KOSTRZEWA, Markus , PUSCH, Wolfgang , MAIER, Thomas , MICHELMANN, Karsten , FRANZEN, Jochen
IPC分类号： C12Q1/04 , G01N33/68 , C12M1/34 , C12M3/00
摘要： The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.

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