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    • 2. 发明公开
    • Modified reductase from Penicillium citrinum and its encoding gene
    • 枸杞子枸杞子
    • EP1378566A2
    • 2004-01-07
    • EP03254141.9
    • 2003-06-30
    • Sumitomo Chemical Company, Limited
    • Asako, HiroyukiShimizu, Masatoshi
    • C12N9/02C12N15/53C12P7/42C12P7/62C12P13/00
    • C12N9/0036C12P7/42C12P7/62
    • There is disclosed a reductase having good heat stability, which results in decrease in reaction time and improvement of reaction efficiency. Specifically, disclosed is an enzyme having an amino acid sequence of SEQ ID NO: 1 in which at least one of the amino acids of the positions 245 and 271 of the amino acid sequence of SEQ ID NO:1 is replaced with another amino acid(s); a polynucleotide having a nucleotide sequence that encodes the amino acid sequence of said reductase; a vector containing said polynucleotide; a transformant containing said polynucleotide or said vector, and the like.
    • 公开了具有良好的热稳定性的还原酶,这导致反应时间的减少和反应效率的提高。 具体地,公开了具有SEQ ID NO:1的氨基酸序列的酶,其中SEQ ID NO:1的氨基酸序列的位置245和271的至少一个氨基酸被替换为另一个氨基酸( 或多个); 具有编码所述还原酶的氨基酸序列的核苷酸序列的多核苷酸; 含有所述多核苷酸的载体; 含有所述多核苷酸或所述载体的转化体等。公开了具有良好的热稳定性的还原酶,这导致反应时间的减少和反应效率的提高。 具体地,公开了具有SEQ ID NO:1的氨基酸序列的酶,其中SEQ ID NO:1的氨基酸序列的位置245和271的至少一个氨基酸被替换为另一个氨基酸( 或多个); 具有编码所述还原酶的氨基酸序列的核苷酸序列的多核苷酸; 含有所述多核苷酸的载体; 含有所述多核苷酸或所述载体的转化体等。