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1. 发明公开

EP1060263A4 METHOD AND COMPOSITIONS FOR DETECTING LUCIFERASE IN BIOLOGICAL SAMPLES 有权
标题翻译：方法和组合物荧光素酶检测生物样品
公开(公告)号：EP1060263A4
公开(公告)日：2005-07-20
申请号：EP99903144
申请日：1999-01-18
申请人： PACKARD INSTRUMENT CO INC
发明人： ROELANT CHRISTIAAN
IPC分类号： G01N21/76 , C12N9/99 , C12Q1/66
CPC分类号： C12Q1/66
摘要： The presence of renilla luciferase alone or both renilla luciferase and firefly luciferase is detected by adding reagent mixture(s) to a biological sample and producing glow luminescence having a duration of at least an hour. In producing luminescence from renilla luciferase alone, a reagent is added comprising coelenterazine, and dithiothreitol (DTT) and EDTA, or functional equivalents of DTT and EDTA. Luminescence from samples containing both firefly luciferase and renilla luciferase is produced by first adding a reagent comprising firefly luciferin, ATP, co-factors necessary for firefly luciferase activity (e.g., Ca and Mg ), dithiothreitol (DTT) or functional equivalents thereof, and AMP. Following measurement of the firefly luciferase, coelenterazine and EDTA, or functional equivalents of EDTA, are added, and the luminescence produced by renilla luciferase is measured.

2. 发明公开

EP1237665A4 PIEZOELECTRIC-DROP-ON-DEMAND TECHNOLOGY 有权
标题翻译：压电式点播技术
公开(公告)号：EP1237665A4
公开(公告)日：2002-11-20
申请号：EP00973512
申请日：2000-10-13
申请人： PACKARD INSTRUMENT CO INC
发明人： PAPEN ROELAND E
IPC分类号： B01L3/02 , B03C1/28 , B08B9/04 , B41J2/17 , B41J2/175 , C40B60/14 , G01N35/00 , G01N35/10 , B08B7/00 , B01L11/00 , B08B3/14 , B08B9/00 , B08B9/02
CPC分类号： B08B9/057 , B01J2219/00378 , B01L3/0268 , B03C1/288 , B41J2/16526 , B41J2/1707 , B41J2/175 , C40B60/14 , G01N35/0098 , G01N2035/1006
摘要： A method and apparatus for cleaning the interior of capillary tubes (62) used to dispense 1 to 100 micron diameter liquid droplets by a piezoelectric transducer (60) surrounding each capillary tube. Magnetic particles are aspirated into the capillary tubes and moved by an exterior magnet to cause deposits on the interior walls to be dislodged and subsequently discharged from the capillary tube. In a preferred embodiment, the magnetic particles are coated with a material capable of binding such deposits.

3. 发明公开

EP0810438A3 Microvolume liquid handling system 失效
标题翻译： Vorrichtung zur Handhabung vonMikroflüssigkeitsmengen
公开(公告)号：EP0810438A3
公开(公告)日：1998-09-30
申请号：EP97108726
申请日：1997-05-30
申请人： PACKARD INSTRUMENT CO INC
发明人： PELC RICHARD E , CHIBUCOS NICHOLAS S , PAPEN ROELAND F , MEYER WILHELM J
IPC分类号： B67D3/00 , B01L3/02 , C40B60/14 , G01F1/48 , G01F3/00 , G01N1/00 , G01N35/00 , G01N35/10
CPC分类号： G01N35/1065 , B01J2219/00378 , B01L3/0268 , C40B60/14 , G01F1/48 , G01F3/00 , G01N2035/00237 , G01N2035/1018 , G01N2035/1039 , G01N2035/1041
摘要： A low volume liquid handling system is described which includes a microdispenser (10) employing a piezoelectric transducer (14) attached to a glass capillary, a positive displacement pump (12) for priming and aspirating transfer liquid (24) into the microdispenser, controlling the pressure of the liquid system, and washing the microdispenser between liquid transfers, and a pressure sensor to measure the liquid system (20) pressure and produce a corresponding electrical signal. The pressure signal is used to verify and quantify the microvolume of transfer liquid (24) dispensed and is used to perform automated calibration and diagnostics on the microdispenser. In another embodiment (Fig.7) of the low volume liquid handling system, a system reservoir (214) is connected with tubing to a pressure control system (218) for controlling the liquid system pressure in the system reservoir. The system reservoir (214) is coupled to one or more microdispensers (212) through a distribution tube having a branched section for each microdispenser. In this embodiment, each microdispenser (212) is coupled to its own flow sensor (244) and microvalve (242) to enable a system controller to respectively measure and control the flow of liquid in the each microdispenser.
摘要翻译：描述了一种低容量液体处理系统，其包括使用附接到玻璃毛细管的压电换能器的微型分配器，用于将转移液体引入和吸入微量分配器的正排量泵，控制液体系统的压力，以及在液体之间洗涤微分液传送和压力传感器来测量液体系统压力并产生相应的电信号。压力信号用于验证和量化分配的转移液体的微量，并用于对微型分散器执行自动校准和诊断。在低体积液体处理系统的另一个实施例中，系统储存器与管道连接到压力控制系统，用于控制系统储存器中的液体系统压力。系统储存器通过具有每个微分配器的分支部分的分配管耦合到一个或多个微分配器。在该实施例中，每个微型分配器连接到其自身的流量传感器和微型阀，以使得系统控制器能够分别测量和控制每个微型分配器中的液体的流动。

4. 发明公开

EP0156801A4 METHOD AND APPARATUS FOR MEASURING RADIOACTIVE DECAY. 失效
标题翻译：方法和设备测量放射性衰变。
公开(公告)号：EP0156801A4
公开(公告)日：1987-01-20
申请号：EP83903332
申请日：1983-09-27
申请人： PACKARD INSTRUMENT CO INC
发明人： VALENTA ROBERT J
IPC分类号： G01T1/178 , G01T1/208 , G01T1/38
CPC分类号： G01T1/208 , G01T1/178 , G01T1/38
摘要： Method and apparatus for counting nuclear scintillations particularly the activity in a specimen containing tritium segregates the pulses which are emitted from a scintillator due on interaction with a nuclear particle from the pulses attributable to random noise. The system uses a single photomultiplier (12), the output of which is split among a two-path circuit (22, 32). A pulse height analyzer (46) provides an output when one path (32) has an integrated output signal of preselected amplitude when the other path (22) output has a preselected number of component photon response pulses occurring in a preselected period of time. The system is operable with many scintillation materials as long as the characteristic output from the scintillator is long relative to the duration of a random noise pulse. The preferred embodiment incorporates a solid scintillator although a liquid material may be substituted if the functional criteria are satisfied. Also discussed is the response of an apparatus in accordance with the present invention if some relatively high energy isotopes such as carbon-fourteen are tested.

5. 发明公开

EP1299708A4 UNIVERSAL MICROPLATE ANALYZER 审中-公开
标题翻译： UNIVERSALER MIKROPLATTENANALYSIERER
公开(公告)号：EP1299708A4
公开(公告)日：2006-03-15
申请号：EP01948407
申请日：2001-06-15
申请人： PACKARD INSTRUMENT CO INC
发明人： BOOKER DAVID D , FISCHER ROBERT E , NEWELL MICHAEL P , KAPPEL DAVID W , MORITZ SCOTT , OLEKSY JEROME E
IPC分类号： G01N21/59 , G01N21/64 , G01N21/01
CPC分类号： G01N21/6452 , B01J2219/00702 , G01N2021/6463 , G01N2021/6484
摘要： A universal microplate analyzer capable of carrying out measurements on samples contained in the wells of microplates (42) by fluorescence, absorbance, luminescence employs at least two light sources (32a, 32b) and optical fiber channels (34a-c) for directing excitation light to the sample wells. Flexibility of operation is provided by arrays of mirrors (22a-c), apertures (44, 56, 58), and polarizers (48) which can be positioned as required for the analysis to be carried out.
摘要翻译：通过荧光，吸光度，发光能够对包含在微孔板（42）的孔中的样品进行测量的通用微孔板分析仪采用至少两个光源（32a，32b）和光纤通道（34a-c）用于引导激发光到样品井。操作的灵活性由可根据需要进行分析的镜子阵列（22a-c），孔（44,56,58）和偏振器（48）提供。

6. 发明公开

EP1163497A4 PRODUCING AND MEASURING LIGHT AND DETERMINING THE AMOUNTS OF ANALYTES IN MICROPLATE WELLS 有权
标题翻译：光产生光测量和产品的数量法测定微盘井
公开(公告)号：EP1163497A4
公开(公告)日：2002-04-24
申请号：EP00915795
申请日：2000-02-18
申请人： PACKARD INSTRUMENT CO INC
发明人： DUEBENDORFER JUERG , JONES DONALD , NEUMANN KENNETH , WANG CHANG
IPC分类号： G01N21/64 , G01J3/30 , G01J1/58 , G01N21/25 , G01N35/02
CPC分类号： G01N21/6452 , G01N2201/0648
摘要： An apparatus for measuring light in samples using a high intensity light source (12), is presented. The system utilizes bifurcated fiber bundle (26) to transmit light at the excitation and emission wavelength bands. It also uses a band-pass filter (36) for eliminating extraneous light, including that which corresponds to the excitation wavelength range, while permitting the emitted light to pass to a detector (41) for quantitation. The system employs a shutter (39) to shield the detector (41) while the laser light source is activated, and a controller to intermittently activate the laser light and close the shutter. The apparatus preferably includes lenses (16, 17, 37) for better illumination and read out conditions. The apparatus is employed in Luminescence Oxygen Channeling Immunoassays. The method has high sensitivity, accuracy and precision, and the apparatus is highly compact. Accordingly, the analyzer can perform assays in nanoliter to microliter sample volumes in standard microplates having at least 96, 384 or 1536 wells.

7. 发明公开

EP1007973A4 MICROVOLUME LIQUID HANDLING SYSTEM 失效
标题翻译： MIKROVOLUMENFLUESSIGKEITSHANDHABUNGSSYSTEM
公开(公告)号：EP1007973A4
公开(公告)日：2000-06-21
申请号：EP98915459
申请日：1998-04-08
申请人： PACKARD INSTRUMENT CO INC
发明人： PELC RICHARD E , CHIBUCOS NICHOLAS S , PAPEN ROELAND F , MAYER WILHELM
IPC分类号： C40B60/14 , G01F1/48 , G01F3/00 , G01N35/00 , G01N35/10 , B01L3/02
CPC分类号： G01N35/1016 , B01J2219/00378 , C40B60/14 , G01F1/48 , G01F3/00 , G01N2035/00237 , G01N2035/1018 , G01N2035/1025 , G01N2035/1039 , G01N2035/1041
摘要： A microvolume liquid handling system includes a microdispenser employing a piezoelectric transducer attached to a glass capillary, a positive displacement pump for priming and aspirating transfer liquid into the microdispenser, controlling the pressure of the liquid system, and washing the microdispenser between liquid transfers, and a pressure sensor to measure the liquid system pressure and produce a corresponding electrical signal. The pressure signal is used to verify and quantify the microvolume of transfer liquid dispensed and is used to perform automated calibration and diagnostics on the microdispenser. In another embodiment of the microvolume liquid handling system, a system reservoir is connected with tubing to a pressure control system for controlling the liquid system pressure in the system reservoir. The system reservoir is coupled to one or more microdispensers through a distribution tube having a branched section for each microdispenser. In this embodiment, each microdispenser is coupled to its own flow sensor and to enable a system controller to respectively measure and control the flow of liquid in the each microdispenser. Dispensing of a single sub-nanoliter drop can be detected in real time. As the result of dispensing the liquid in sub-nanoliter droplets, the dispensed volume can be precisely controlled. The dispenser automatically detects the liquid surface of the transfer liquid, automatically aspirate, analyze desired volume of the transfer liquid, dispense the transfer liquid without contacting the destination vessel or its contents, and automatically wash off the transfer liquid from dispensing system after each transfer. This system is capable of automatically sensing liquid surfaces, aspirating liquid to be transferred, and then dispensing small quantities of liquid with high accuracy, speed and precision. The system is pulsated at high frequency to prevent or eliminate clogging. Immiscible liquid between the transfer liquid and the system liquid reduces the required amount of transfer liquid needed for dispensing.
摘要翻译：微量液体处理系统包括使用连接至玻璃毛细管的压电换能器的微分配器，用于将转移液体灌注并吸入微量分配器中的正排量泵，控制液体系统的压力以及在液体转移之间清洗微分配器，压力传感器来测量液体系统压力并产生相应的电信号。压力信号用于验证和量化分配的转移液体的微量，并用于对微分配器进行自动校准和诊断。在微体积液体处理系统的另一个实施例中，系统容器通过管连接到用于控制系统容器中的液体系统压力的压力控制系统。系统贮存器通过具有用于每个微分配器的分支部分的分配管与一个或多个微分配器连接。在该实施例中，每个微分配器耦合到其自己的流量传感器并且使系统控制器能够分别测量和控制每个微分配器中的液体流量。可以实时检测单次亚纳升滴的分配。由于以亚纳升液滴分配液体，可以精确控制分配体积。分配器自动检测转印液体的液面，自动吸取，分析转印液体的所需体积，分配转印液体而不接触目的容器或其内容物，并在每次转印后自动从分配系统清洗转印液体。该系统能够自动检测液体表面，吸取要传送的液体，然后以高精度，高速度和精确度分配少量液体。系统以高频脉动，以防止或消除堵塞。转印液体和系统液体之间的不混溶液体减少了分配所需的转印液体量。

8. 发明公开

EP1181099A4 DISPENSING LIQUID DROPS ONTO POROUS BRITTLE SUBSTRATES 无效
标题翻译：供应液滴在多孔基材上SPRÖDBRÜCHIGE
公开(公告)号：EP1181099A4
公开(公告)日：2002-09-04
申请号：EP01942582
申请日：2001-01-11
申请人： PACKARD INSTRUMENT CO INC
发明人： PAPEN ROELAND E
IPC分类号： B01L3/02 , C40B60/14 , G01F1/48 , G01F3/00 , G01N35/00 , G01N35/10 , B01L3/00 , G01N1/10
CPC分类号： B01L3/0268 , B01J2219/00378 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/0063 , B01J2219/00659 , B01J2219/00689 , B01J2219/00691 , C40B60/14 , G01F1/48 , G01F3/00 , G01N35/1016 , G01N35/1065 , G01N2035/00237 , G01N2035/1018 , G01N2035/1025 , G01N2035/1034 , G01N2035/1039 , G01N2035/1041 , Y10T436/2575
摘要： A system for aspirating and ejecting microvolume drops (26) of liquid onto porous sites of a substrate wafer includes a microdispenser (16) employing a piezoelectric transducer (60) attached to a glass capillary (62), a means for priming and aspirating transfer liquid (24) into the microdispenser (16), for controlling the pressure of the system liquid (20), and for washing the microdispenser (16) between liquid transfers, and a pressure sensor (14) to measure the system liquid pressure and produce a corresponding electrical signal. The drops are generally in the 10 to 100 micron range and the pores are generally 10 to 10,000 times smaller than the diameter of the drops deposited thereon. The resulting spots are uniform, and only slightly larger in diameter of the drops. The drops are ejected from a distance greater than the diameter of the drops, thus avoiding any contact with the dispenser that could damage the wafer. The system detects dispensing of a drop onto the reaction site.

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