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热词
    • 2. 发明公开
    • Detection of microbes in a sample
    • Nachweis von Mikroben in einer Probe。
    • EP0254771A2
    • 1988-02-03
    • EP86117754.1
    • 1986-12-19
    • Edberg, Stephen C.
    • Edberg, Stephen C.
    • C12Q1/04
    • C12Q1/10C12Q1/04C12Q1/34
    • The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can meta­bolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.
    • 通过向样品中加入测试培养基来确定样品中预定目标微生物的存在或不存在,反之亦然。 测试培养基为目标微生物提供选择性生长培养基,并且包括只有目标微生物可代谢的特定营养物质。 通过将样品改变部分附接到其上来改变该特定营养物,从而将营养物转化为营养指示剂。 只有当特定营养物质被目标微生物代谢时,样品改变部分被激活才能改变样品。 样品改变部分可以是改变样品的颜色(可见或不可见)或改变样品的电特性或改变样品的一些其它可检测特征的材料。 测试介质不必保持无菌状态,测试程序不必在无菌环境中进行。