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81. 发明公开

EP1155151A1 METHODS FOR DETERMINING THE PRESENCE OF NUCLEIC ACID TARGET SEQUENCES AND APPLICATIONS THEREOF 审中-公开
标题翻译：用于确定NUCLEIC靶序列及其用途
公开(公告)号：EP1155151A1
公开(公告)日：2001-11-21
申请号：EP00911863.9
申请日：2000-02-18
申请人： PROMEGA CORPORATION
发明人： SHULTZ, John, W. , LEWIS, Martin, K. , LEIPPE, Donna , MANDREKAR, Michelle , KEPHART, Daniel , RHODES, Richard, B. , ANDREWS, Christine, Ann , HARTNETT, James, R. , GU, Trent , OLSON, Ryan, J. , WOOD, Keith, V. , WELCH, Roy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/66 , C12Q1/6823 , C12Q1/6827 , Y10T436/24 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.

82. 发明公开

EP0803058A4 FLUOROMETRIC ASSAY FOR DETECTING NUCLEIC ACID CLEAVAGE 失效
标题翻译：荧光测试为进行裂解NUCLEIC的检测
公开(公告)号：EP0803058A4
公开(公告)日：2001-08-16
申请号：EP95944537
申请日：1995-12-27
申请人： UNIV GEORGETOWN
发明人： HAN MYUN KI , LEE S PAUL , CHIRIKJIAN JACK G
IPC分类号： C12Q1/44 , C12Q1/68 , G01N21/64 , C12P19/34 , G01N21/76 , G01N33/53 , G01N33/533
CPC分类号： C12Q1/6818 , C12Q1/44 , C12Q1/68 , C12Q1/6823 , C12Q2563/107 , C12Q2521/301 , C12Q2565/101
摘要： A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

83. 发明公开

EP1049802A1 FLUORIMETRIC DETECTION SYSTEM OF A NUCLEIC ACID 有权
标题翻译：荧光系统，对核酸的检测
公开(公告)号：EP1049802A1
公开(公告)日：2000-11-08
申请号：EP98956993.4
申请日：1998-11-27
申请人： The Secretary of State for Defence , Bio/gene Limited
发明人： LEE, Martin, Alan , FUERST, RoderickBio/gene Limited
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6823 , Y10S436/80 , Y10T436/143333 , C12Q2563/107 , C12Q2561/113 , C12Q2527/107 , C12Q2563/173
摘要： A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: (a) adding to a sample suspected of containing said target nucleic acid sequence, a probe specific for said target sequence and DNA duplex binding agent, said probe comprising a reactive molecule able to absorb fluorescence from or donate fluorescent energy to said DNA duplex binding agent, (b) subjecting the thus formed mixture to an amplification reaction in which target nucleic acid is amplified, (c) subjecting said sample to conditions under which the said probe hybridises to the target sequence, and (d) monitoring fluorescence from said sample. This method can be used for example to monitor amplification reactions such as PCR reactions, such that the amount of target sequence present in the sample may be determined. Additionally or alternatively, it may be used to generate duplex destabilisation data such as melt hysteris information for amplification monitoring or for detection and quantitation of polymorphisms or allelic variation, and so is useful in genetic diagnosis.

84. 发明公开

EP1029077A2 SPEZIFISCHES UND EMPFINDLICHES NUKLEINSÄURENACHWEISVERFAHREN 审中-公开
标题翻译：特异性和敏感性NUCLEIC检测方法
公开(公告)号：EP1029077A2
公开(公告)日：2000-08-23
申请号：EP98955529.7
申请日：1998-11-03
申请人： Roche Diagnostics GmbH
发明人： KESSLER, Christoph , HABERHAUSEN, Gerd , BARTL, Knut , ORUM, Henrik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid comprising the production of a plurality of amplifications of a section of said nucleic acid with the assistance of two primers of which one can bond on a bonding sequence A of the nucleic acid and the other can bond on a bonding sequence C' which is complimentary to a sequence C. Sequence C does not overlap A and is situated in a 3' direction from A. The inventive method also includes bringing the amplifications in contact with a probe having a bonding sequence D which can bond on a sequence B, said sequence B being situated between sequences A and C, or the complement thereof. In addition, the invention relates to the detection of the construction of a hybrid out of the amplification and the probe, whereby the sequence situated between the bonding sequences A and C contains no nucleotides, said nucleotides not being linked to the bonding sequence D of the probe or to complement D' thereof.

85. 发明公开

EP1016731A1 PROBES FOR DETECTING TARGET NUCLEIC ACID, METHOD OF DETECTING TARGET NUCLEIC ACID, AND SOLID PHASE FOR DETECTING TARGET NUCLEIC ACID AND PROCESS FOR PRODUCING THE SAME 审中-公开
标题翻译：探针 - 靶核酸的测定，测定方法的靶核酸，固相的靶核酸和制造方法的测定
公开(公告)号：EP1016731A1
公开(公告)日：2000-07-05
申请号：EP99900152.2
申请日：1999-01-08
申请人： Laboratory of Molecular Biophotonics
发明人： ABE, Satoshi, Laboratory of Molecular Biophotonics , KODAMA, Hirofumi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6862 , C12Q1/6823 , C12Q1/6834 , C12Q2565/543 , C12Q2521/501 , C12Q2523/107 , C12Q2521/101
摘要： This invention relates to a pair of probes for the detection of a target nucleic acid, said pair of probes comprising (1) probe 1 having base sequence B1 complementary to A1 between two specific sequential base sequences A1 and A2 of the nucleic acid, wherein (a) the 5'-terminus of base sequence B1 is phosphorylated and (b) a first solid phase immobilizing part is bound to the 3'-terminus of base sequence B1, and (2) probe 2 having base sequence B2 complementary to A2 between base sequences A1 and A2, wherein (a) the 5'-terminus of base sequence B2 is bound to one end of a cleavage part and (b) a second solid phase immobilizing part is bound to the other part of the cleavage part. When the target nucleic acid is hybridized above the solid phase of the invention where said probes are immobilized on its surface, the probes on the solid phase occupy spatial positions beneficial to the formation of a hybrid and thus forms the hybrid efficiently. Therefore, probe 1 and probe 2 are efficiently ligated by ligase reaction. Furthermore, after the target nucleic acid is removed from the hybrid, only probe 2 ligated as described above will be able to exist on the solid phase through cleavage reaction of the cleavage part. Therefore, after the free probe 2 has been removed by washing, it will become possible to detect the presence of probe 2 on the solid phase ligated as described above, with high sensitivity and high recognition. This will enable detection of the presence of a target nucleic acid with higher sensitivity and higher recognition as compared to methods in the prior art.
摘要翻译：本发明涉及到一对探针的用于检测靶核酸的，所述一对探针，其包括核酸，worin的两个特定顺序的碱基序列A1和A2之间的（1）探针具有1碱基序列A1互补B1的（一个）的碱基序列B1的5'末端被磷酸化和（b）第一固相固定部分结合到碱基序列B1的3'末端，和（2）样品2具有碱基序列B2互补A2之间碱基序列A1和A2，worin的（a）碱基序列B2的5“末端结合到切割部分的一端和（b）第二固相固定部分结合到切割部分的另一部分。当靶核酸，其中所述探针在其表面上固定化本发明的固相以上的杂交，在固相上探针占据的杂交体的形成有利的空间位置，从而有效地形成混合。因此，探针1和探针2被有效地通过连接酶反应连接。进一步，在靶后核酸从混合去除，只有两个测试连接如上所述将能够通过切割部分的切割反应在固相存在。因此，免费试用2已经通过洗涤除去后，将成为能够检测上如上述那样，具有高灵敏度和高识别连接在固相样品2的存在。这将使一个靶核酸与更高的灵敏度和更高的识别的存在的检测相比，在现有技术中的方法。

86. 发明公开

EP1015632A1 CHARACTERISING NUCLEIC ACID BY MASS SPECTROMETRY 审中-公开
标题翻译：表征NUCLEIC以质量
公开(公告)号：EP1015632A1
公开(公告)日：2000-07-05
申请号：EP98942924.6
申请日：1998-09-15
申请人： Brax Group Limited
发明人： SCHMIDT, Günter , THOMPSON, Andrew, Hugin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6872 , C12Q1/6823 , C12Q2563/167 , C12Q2537/157
摘要： The present invention provides a method for analysing a population of nucleic acid fragments each labelled with a mass label, which method comprises: i) ionising the population; ii) sorting the ionised population in a mass spectrometer according to mass into sub-populations each containing at least one labelled fragment; iii) cleaving each sub-population to release the mass label associated with each labelled fragment; iv) determining the mass of each released mass label by mass spectroscopy; and v) assigning each mass label to its associated fragment.

87. 发明公开

EP1015626A1 METHOD OF MONITORING THE COMPETITIVENESS OF A MICROBIAL STRAIN 审中-公开
标题翻译： PROCEDURE用于监测竞争力的微生物菌株
公开(公告)号：EP1015626A1
公开(公告)日：2000-07-05
申请号：EP98940492.6
申请日：1998-09-11
申请人： Microtag AS
发明人： ALESTROM, Peter , LILLEHAUG, Dag , VEGARUD, Gerd
IPC分类号： C12Q1/04 , C12Q1/68 , C12N1/00 , C12N1/20 , C12N1/21 , A23C9/127 , A23C19/032
CPC分类号： C12Q1/6888 , A23C19/0321 , A23C19/0323 , A23C2220/202 , C12N15/10 , C12Q1/6809 , C12Q1/6823 , C12Q2600/158
摘要： A method of selectively monitoring development and/or competitiveness of a microbial strain in a sample environment, comprising inserting a selectively detectable genetic label into the strain and adding the labelled strain to the sample environment and isolating DNA or RNA of said strain from the sample environment and detecting the amount of the genetic label. The method is also used for selecting, among a multiplicity of microbial strains, the strain that in a pre-selected environment and under pre-selected conditions has the highest capability to survive, multiply and/or express a pre-selected gene. The amount of genetic label is detected by PCR including a PCR technique that is capable of detecting a label consisting of a single nucleotide substitution, including a technique where the first PCR cycle involves the use of a reverse transcriptase. The method is also useful for establishing the identity and origin of a microbial strain by inserting a genetic label comprising a coded message.

88. 发明公开

EP0951565A1 IMPROVED PROBING OF SPECIFIC NUCLEIC ACIDS 失效
标题翻译：特定碱基的完善的检测
公开(公告)号：EP0951565A1
公开(公告)日：1999-10-27
申请号：EP97921072.0
申请日：1997-04-30
申请人： Landegren, Ulf
发明人： Landegren, Ulf
IPC分类号： C12N15 , C12Q1
CPC分类号： C12Q1/6823 , C12Q1/6827 , C12Q1/6834 , C12Q2533/107 , C12Q2525/307 , C12Q2525/313 , C12Q2563/131 , C12Q2523/107 , C12Q2521/501
摘要： The present invention relates to improved methods for probing of specific nucleic acids using circularizable probes designed such that they report the presence of a target sequence by allowing a detectable moiety to remain bound if and only if the probe has been cyclized in a target-dependent linking reaction. The invention may be used for distinction between sequence specific variations of nucleic acids.

89. 发明公开

EP0914471A2 METHOD FOR DISSOCIATING BIOTIN COMPLEXES 失效
标题翻译：方法解离素复合物作者：
公开(公告)号：EP0914471A2
公开(公告)日：1999-05-12
申请号：EP97926469.0
申请日：1997-05-13
申请人： SEQUENOM, INC.
发明人： JURINKE, Christian , VAN DEN BOOM, Dirk , KÖSTER, Hubert
IPC分类号： C12N15 , C12Q1 , G01N33
CPC分类号： G01N33/53 , C12Q1/6823 , C12Q1/6834 , C12Q1/6869 , C12Q2565/627 , C12Q2563/131 , C12Q2565/518
摘要： A method for dissociating a complex of a biotin compound and a biotin-binding compound, by contacting the complex with an amine, is disclosed.

90. 发明公开

EP0688366A4 SENSITIVE NUCLEIC ACID SANDWICH HYBRIDIZATION ASSAYS AND KITS 失效
标题翻译：敏感NUCLEIC三明治杂交法和试剂盒
公开(公告)号：EP0688366A4
公开(公告)日：1999-04-21
申请号：EP94906682
申请日：1994-01-14
申请人： NEW YORK HEALTH RES INST
发明人： TYAGI SANJAY , LANDEGRAN ULF D , LIZARDI PAUL M , KRAMER FRED R , BLOK HERMAN J
IPC分类号： G01N33/53 , C07H21/04 , C12N15/09 , C12Q1/34 , C12Q1/68 , G01N33/566 , G01N33/58 , C12P19/34
CPC分类号： C12Q1/6867 , C12Q1/6813 , C12Q1/682 , C12Q1/6823 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2521/501 , C12Q2521/337
摘要： Nucleic acid sandwich hybridization assays are provided that incorporate one or a combination of background reduction steps. Those steps include use of a separate capture probe and separation from immobilized capture probes by cleavage and isolation. A very sensitive assay for RNA targets includes both of those steps, plus RNA binary probes, and RNA-directed RNA ligase and amplification by an RNA-directed RNA polymerase. Kits of reagents for performing assays according to this invention are also provided.

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