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71. 发明公开

EP1329509A3 Method for generating a subtracted cDNA library and uses of the generated library 失效
标题翻译：形成消减cDNA文库和它们的使用方法
公开(公告)号：EP1329509A3
公开(公告)日：2003-10-15
申请号：EP03006745.8
申请日：1994-10-24
申请人： The Trustees of Columbia University in the City of New York
发明人： Fisher, Paul B. , Jiang, Hongping
IPC分类号： C12N15/12 , C12N15/10 , C07K14/54 , C12Q1/68 , G01N33/574 , G01N33/68
CPC分类号： C07K14/54 , C07K2319/00 , C12N15/1072 , C12N15/1096 , C12Q1/6809 , C12Q1/6886 , C12Q2600/112 , C12Q2600/136 , C12Q2600/158 , G01N33/57407 , G01N33/57426 , G01N33/5743 , G01N33/6866 , G01N2333/705
摘要： This invention provides a method of generating a subtracted cDNA library of a cell comprising: a) generating a cDNA library of the cell: b) isolating double-stranded DNAs from the cDNA library: c) releasing the double-stranded cDNA inserts from the double-stranded DNAs: d) denaturing the isolated double-stranded cDNA inserts: e) hybridizing the denatured double-stranded cDNA inserts with a labelled single-stranded nucleic acid molecules which are to be subtracted from the cDNA library: and f) separating the hybridized labeled single-stranded nucleic acid molecule from the double-stranded cDNA inserts, thereby generating a subtracted cDNA library of a cell. This invention also provides different uses of the subtracted library.

72. 发明公开

EP1343908A2 NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸扩增
公开(公告)号：EP1343908A2
公开(公告)日：2003-09-17
申请号：EP01993343.1
申请日：2001-12-21
申请人： Arcturus Engineering, Inc.
发明人： ERLANDER, Mark, G. , SALUNGA, Ranelle, C.
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12N15/1096 , C12Q1/6865 , C12Q2525/173 , C12Q2525/143
摘要： The present invention provides methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

73. 发明公开

EP1320596A1 METHOD OF PERFORMING REVERSE TRANSCRIPTION REACTION USING REVERSE TRANSCRIPTASE ENCODED BY NON-LTR RETROTRANSPOSABLE ELEMENT 审中-公开
标题翻译： FOR逆转录酶反应中使用一项所述的非LTR反转录转座元件的程序的执行CODED逆转录酶
公开(公告)号：EP1320596A1
公开(公告)日：2003-06-25
申请号：EP01966453.1
申请日：2001-08-30
申请人： University of Rochester
发明人： EICKBUSH, Thomas, H. , BIBILLO, Arkadiusz
IPC分类号： C12N15/10 , C12N15/11 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6844 , C12N9/1276 , C12N15/1096 , C12Q2521/107
摘要： The present invention relates to a method of preparing a cDNA molecule which includes: contacting an RNA molecule, in the presence of dNTPs, with a non-LTR retrotransposon protein or polypeptide having reverse transcriptase activity under conditions effective for production of a cDNA molecule complementary to the RNA molecule, said contacting being carried out in the absence of a target DNA molecule of the non-LTR retrotransporon protein or polypeptide; and isolating the cDNA molecule.

74. 发明公开

EP1090996A4 VECTOR EXPRESSING THE FULL-LENGTH GENE OF RNA VIRUS AND USE THEREOF 审中-公开
标题翻译： VELTOR，完整的RNA病毒基因的表达，其用途
公开(公告)号：EP1090996A4
公开(公告)日：2003-03-26
申请号：EP99926798
申请日：1999-06-24
申请人： CHUGAI PHARMACEUTICAL CO LTD
发明人： KOHARA MICHINORI , KOHARA KYOKO , TAIRA KAZUNARI , MATSUZAKI JUNICHI , OHMORI HIROSHI
IPC分类号： C07K14/18 , C12N7/00 , C12N15/10 , C12N15/40 , C12N15/51 , C12N15/66 , A01K67/027 , C12N5/16 , C12Q1/70
CPC分类号： C07K14/005 , C12N7/00 , C12N15/1096 , C12N15/66 , C12N2770/24221 , C12N2770/24222
摘要： A vector containing a cDNA which encodes an RNA virus gene and having been constructed so as to ensuring exact and homogeneous transcription of both terminals of the RNA virus gene. This vector is useful in clarifying the mechanism of the replication of the RNA virus, clarifying the mechanism of the onset of the infection with the RNA virus, developing remedies and therapeutic means therefor, etc.

75. 发明公开

EP1275734A1 Method for random cDNA synthesis and amplification 审中-公开
标题翻译： Verfahren zurzufälligeSynthese und AmplifikationkomplementäresDNA
公开(公告)号：EP1275734A1
公开(公告)日：2003-01-15
申请号：EP01115787.2
申请日：2001-07-11
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： Brehm, Ursula , Eichner, Cornelia , Wartbichler, Regina , Frey, Bruno, Dr.
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1096 , C12Q1/6853 , C12Q2600/158 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143
摘要： The new invention is directed to a method for amplification of a pool of RNA sequences, comprising (I) synthesis of total first strand cDNA with a first primer comprising a first segment located at the 3' terminal part of said oligonucleotide which is capable of hybridizing to substantially all RNAs contained in the RNA pool and a second segment, said second segment being located more proximal to the 5' end of said oligonucleotide, said second segment being capable of serving as a primer binding side for another nucleic acid amplification primer by itself and (II) synthesis of total second strand cDNA with a second primer, said second primer comprising a first randomized segment located at the 3' end of said primer and a second segment which is located more proximal to the 5' end of said oligonucleotide, said second segment being capable of serving as a primer binding side by itself for a nucleic acid amplification primer such that a double stranded cDNA is generated.
摘要翻译：本发明涉及一种用于扩增RNA序列池的方法，其包括（I）总第一链cDNA的合成，第一引物包含位于所述寡核苷酸的3'端部分的第一区段，其能够杂交基本上包含在所述RNA库中的所有RNA和第二片段，所述第二片段位于所述寡核苷酸的5'末端更靠近的位置，所述第二片段能够自身用作另一核酸扩增引物的引物结合侧和（II）用第二引物合成总第二链cDNA，所述第二引物包含位于所述引物3'末端的第一个随机区段和位于更靠近所述寡核苷酸5'末端的第二区段，所述第二片段本身可以用作核酸扩增引物的引物结合侧，从而产生双链cDNA。

76. 发明公开

EP1250420A2 TECHNIQUES FOR GROWTH AND DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS 审中-公开
标题翻译：方法对人类多能干细胞的增殖和分化
公开(公告)号：EP1250420A2
公开(公告)日：2002-10-23
申请号：EP01900997.6
申请日：2001-01-10
申请人： Geron Corporation
发明人： CARPENTER, Melissa, K. , FUNK, Walter, D. , GOLD, Joseph, D. , INOKUMA, Margaret, S. , XU, Chunhui
IPC分类号： C12N5/06 , C12N5/08 , G01N33/50 , C07H21/00 , C12N5/10 , C12P19/34 , C12N15/10 , C12P21/02 , C07K16/18
CPC分类号： C12N5/0606 , A61K35/12 , C12N15/1034 , C12N15/1072 , C12N15/1096 , C12N2500/14 , C12N2500/30 , C12N2500/38 , C12N2500/44 , C12N2500/62 , C12N2501/105 , C12N2501/13 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/999 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2510/00 , C12N2510/04 , C12N2533/90
摘要： This disclosure provides an improved system for culturing human pluripotent stem (pPS) cells in the absence of feeder cells. The role of the feeder cells can be replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. Permanent cell lines are provided that can produce conditioned medium on a commercial scale. Methods have also been discovered to genetically alter pPS cells by introducing the cells with a viral vector or DNA/lipid complex. The system described in this disclosure allows for bulk proliferation of pPS cells for use in studying the biology of pPS cell differentiation, and the production of important products for use in human therapy.

77. 发明公开

EP1243650A3 Process for constructing a cdna library, and a polypeptide and dna coding for the same 失效
标题翻译：一种用于cDNA文库的制备和多肽及其相应的DNA过程
公开(公告)号：EP1243650A3
公开(公告)日：2002-10-02
申请号：EP02008698.9
申请日：1994-01-14
申请人： Honjo, Tasuku , ONO PHARMACEUTICAL CO., LTD.
发明人： Honjo, Tasuku , Tashiro, Kei , Tada, Hideaki, c/o Ono Pharmaceutical Co.,Ltd.
IPC分类号： C12N15/10 , C12N15/12 , C12N15/62 , C07K14/47 , C07K16/18 , C12Q1/68 , A61K38/17
CPC分类号： C12N15/1051 , A61K38/00 , C07K14/475 , C07K14/70567 , C07K14/715 , C07K2319/02 , C07K2319/75 , C12N15/1093 , C12N15/1096 , C12N15/625 , C12Q1/6853
摘要： A process for constructing a cDNA library which has a selectivity for signal peptides, that makes it possible to efficiently identify unknown and useful polypeptide comprising a signal peptide. A novel polypeptide consisting of 89 amino acids (including a signal peptide) produced by a stroma cell line, which is useful as an agent for preventing or treating, for example, anemia, leukopenia or infections and the like, and DNAs coding for said polypeptide, have been identified using the process of the invention.

78. 发明公开

EP1229134A2 Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell 审中-公开
标题翻译：识别核酸序列影响使用转录后基因沉默的其中的细胞的功能
公开(公告)号：EP1229134A2
公开(公告)日：2002-08-07
申请号：EP02250681.0
申请日：2002-01-31
申请人： Nucleonics, Inc , Message Pharmaceuticals, Inc.
发明人： Giordano, Tony , Pachuk, Catherine , Satishchandran, Chandrasekhar
IPC分类号： C12Q1/68 , C12N15/10 , G01N33/50
CPC分类号： C12N15/1086 , C12N15/1096 , C12Q1/6897
摘要： Described herein are methods for identifying nucleic acid sequences that modulate the function of a cell, the expression of a gene in a cell, or the biological activity of a target polypeptide in a cell. The methods involve the use of double stranded RNA expression libraries, double stranded RNA molecules, and post-transcriptional gene silencing techniques.
摘要翻译：用于鉴定核酸序列描述于一些方法做了调制单元的功能，一个基因在细胞中的表达，或在细胞中的靶多肽的生物活性。该方法涉及使用双链RNA表达文库，双链RNA分子，和转录后基因沉默技术。

79. 发明授权

EP0735144B1 Method for molecular indexing of genes using restriction enzymes 失效
标题翻译：使用限制性内切酶基因的分子索引方法
公开(公告)号：EP0735144B1
公开(公告)日：2002-06-05
申请号：EP96104817.0
申请日：1996-03-26
申请人： Japan Science and Technology Corporation
发明人： Kato, Kikuya
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6809 , C12N15/1096 , C12Q1/6855 , C12Q2537/149 , C12Q2521/313

80. 发明公开

EP1137812A4 DNA-PROTEIN FUSIONS AND USES THEREOF 有权
标题翻译： DNA蛋白合并及的相同的应用程序
公开(公告)号：EP1137812A4
公开(公告)日：2002-04-24
申请号：EP99967171
申请日：1999-12-02
申请人： PHYLOS INC
发明人： LOHSE PETER , KURZ MARKUS , WAGNER RICHARD
IPC分类号： C12N15/09 , C07B61/00 , C07H19/20 , C07H21/00 , C07K9/00 , C07K14/00 , C07K17/02 , C07K19/00 , C12M1/00 , C12N15/10 , C12P21/02 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12N15/1096 , C07B2200/11 , C07H19/20 , C07H21/00 , C07K14/00 , C12N15/1062 , C12N15/1075 , C40B30/04 , C40B40/00 , C40B40/08 , C40B40/10 , C40B50/14
摘要： The invention provides methods for covalently tagging proteins with their encoding DNA sequences as shown in the Figure. These DNA-protein fusions may be used in molecular evolution and recognition techniques.

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