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71. 发明公开

EP1456226A1 SITE SELECTIVE ACYLATION 审中-公开
标题翻译： MAKE选择性酰化
公开(公告)号：EP1456226A1
公开(公告)日：2004-09-15
申请号：EP02783948.9
申请日：2002-11-18
申请人： ModPro AB
发明人： BALTZER, Lars
IPC分类号： C07K1/10 , C07K14/00
CPC分类号： C07K1/1072
摘要： Disclosed are novel polypeptides consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, wherein either both have a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5 or SEQ. ID. No. 7, or one has a sequences according to SEQ. ID. No. 6, and the other one has a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5 or SEQ. ID. No. 7. Also disclosed is a method for site-selective acylation of a folded polypeptide or protein based on the use of a four helix bundle formed of two dimerized helix-loop-helix motifs folded in an antiparallel mode, said helix-loop-helix motifs comprising amino acid residues in a heptad repeat pattern a b c d e fgn, wherein all but 1-3 of said amino acid residues in positions a and d are non-polar and wherein said 1-3 amino acid residues in positions a and d that are non-polar, are selected from the group consisting of lysine, ornithine, diaminobutyric acid and homolysine, wherein said amino acid residues in positions a and d form a hydrophobic core, wherein the polypeptide and/or protein to be acylated is placed in an aqueous solution and an acylation agent is added. Furthermore, use of said method is disclosed.

72. 发明公开

EP1399465A4 LIGATION METHOD AND REAGENTS TO FORM AN AMIDE BOND 有权
标题翻译： LIGATIONSVERFAHREN UND REAGENZIEN ZUR BILDUNG EINER AMID-BINDUNG
公开(公告)号：EP1399465A4
公开(公告)日：2004-03-31
申请号：EP01935432
申请日：2001-05-11
申请人： WISCONSIN ALUMNI RES FOUND
发明人： RAINES RONALD T , KIESSLING LAURA L , NILSSON BRADLEY L
IPC分类号： C07K1/06 , C07F9/48 , C07F9/50 , C07K1/08 , C07K1/107 , C07K1/12 , C07K1/13 , C07K1/00
CPC分类号： C07K1/082 , C07F9/4866 , C07F9/5004 , C07F9/5022 , C07F9/5054 , C07K1/1072 , C07K1/1077
摘要： Methods and reagents for the formation of amide bonds between an activated carboxylic acid derivative and an azide useful in the synthesis of peptides, proteins and derivatized or labeled amino acids, peptide or proteins. The method involves the formation of a phosphinothioester which reacts with an azide resulting in amide formation. The invention provides phosphinothiol reagents which convert activated carboxylic acid derivatives to phosphinothioesters which then react with azides to form an amide bond. The methods and reagents of the invention can be used for stepwise synthesis of peptides on solid supports or for the ligation to two or more amino acids, two or more peptide or two or more protein fragments.
摘要翻译：用于在活性羧酸衍生物和叠氮化物之间形成酰胺键的方法和试剂，用于合成肽，蛋白质和衍生或标记的氨基酸，肽或蛋白质。该方法包括形成与叠氮化物反应导致酰胺形成的膦硫代酯。本发明提供了将活化的羧酸衍生物转化成膦酰硫酯的膦硫醇试剂，然后膦酰硫酯随后与叠氮化物反应形成酰胺键。本发明的方法和试剂可用于在固体支持物上逐步合成肽或用于连接两个或更多个氨基酸，两个或更多个肽或两个或更多个蛋白质片段。

73. 发明公开

EP1399465A2 LIGATION METHOD AND REAGENTS TO FORM AN AMIDE BOND 有权
标题翻译：连接方法和试剂形成酰胺键
公开(公告)号：EP1399465A2
公开(公告)日：2004-03-24
申请号：EP01935432.3
申请日：2001-05-11
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人： RAINES, Ronald, T. , KIESSLING, Laura, L. , NILSSON, Bradley, L.
IPC分类号： C07K1/00
CPC分类号： C07K1/082 , C07F9/4866 , C07F9/5004 , C07F9/5022 , C07F9/5054 , C07K1/1072 , C07K1/1077
摘要： Methods and reagents for the formation of amide bonds between an activated carboxylic acid derivative and an azide useful in the synthesis of peptides, proteins and derivatized or labeled amino acids, peptide or proteins. The method involves the formation of a phosphinothioester which reacts with an azide resulting in amide formation. The invention provides phosphinothiol reagents which convert activated carboxylic acid derivatives to phosphinothioesters which then react with azides to form an amide bond. The methods and reagents of the invention can be used for stepwise synthesis of peptides on solid supports or for the ligation to two or more amino acids, two or more peptide or two or more protein fragments.

74. 发明公开

EP1283872A2 CATALYTICALLY ACTIVE PEPTIDES 有权
标题翻译：催化活性肽
公开(公告)号：EP1283872A2
公开(公告)日：2003-02-19
申请号：EP01930379.1
申请日：2001-05-07
申请人： A+ Science Invest AB
发明人： BALTZER, Lars
IPC分类号： C12N9/10 , C07K1/10 , C07K14/00
CPC分类号： C07K1/1136 , C07K1/006 , C07K1/1072 , C07K1/1077
摘要： A catalytically active peptide comprising an imidazole function in position i flanked by at least one functional group to be amidated in position i+3+4k, where k is an integer equal to or higher than -1 or in position i-4-4n, wherein n is an integer equal to or higher than 0, characterized in that it also comprises at least one activating group in position i+4+4n or i-3-4n, respectively, wherein n is as above.

75. 发明公开

EP0792357A4 CHEMICAL MODIFICATION OF REPETITIVE POLYMERS TO ENHANCE WATER SOLUBILITY 失效
标题翻译：化学变化，为了提高水的重复聚合物
公开(公告)号：EP0792357A4
公开(公告)日：1999-05-19
申请号：EP95938187
申请日：1995-09-29
申请人： PROTEIN POLYMER TECHNOLOGIES
发明人： STEDRONSKY ERWIN R
IPC分类号： C07K1/107 , C07K14/00 , C07K14/435 , C07K14/78 , C09D189/00 , C12N15/11
CPC分类号： C07K1/1072 , C07K14/001 , C07K14/435
摘要： Highly repetitive proteins which are relatively insoluble in water are chemically modified to increase solubility. The protein is reacted with a functionalizing agent to introduce additional polar functionalities and disrupt the order of the protein. The solubility of the protein in water is increased by the chemical modification, while adhesive and surfactant properties are retained.

76. 发明公开

EP0322262A1 Protéine basique dénommée phospholipase A2 isolée de venin de serpent de la famille des élapidés et sa séquence en amino-acides, dérivés et fragments de ladite protéine, leur procédé d'obtention, compositions thérapeutiques et agents de diagnostic contenant ladite protéine et/ou ses dérivés et/ou ses fragments 失效
标题翻译：获得碱性蛋白磷脂酶A2来自属于家庭眼镜蛇科，其氨基酸序列的蛇的毒液中分离，衍生物和该蛋白质的片段，方法，使它，治疗组合物和诊断装置，包含所有这种蛋白质和/或它们的衍生物和/或它的片段。
公开(公告)号：EP0322262A1
公开(公告)日：1989-06-28
申请号：EP88402884.6
申请日：1988-11-17
申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE
发明人： Menez, André , Chwetzoff, Serge
IPC分类号： C07K13/00 , C07K7/06 , C12N9/20 , G01N33/68 , A61K37/02
CPC分类号： C07K1/1072 , C07K14/46 , C12N9/18
摘要： Protéine basique isolée de venin de serpent de la famille des Elapidés notamment d'un serpent Naja et plus particulièrement de Naja nigricollis et/ou de Naja mossambica pallida, dénommée phospholipase A2 (PLA2), fragments et dérivés de ladite protéine, leurs procédés d'obtention, des compositions pharmaceutiques utilisables en médecine humaine et/ou vétérinaire, ainsi que des agents de diagnostic contenant ladite protéine et/ou ses dérivés et/ou ses fragments.
Ladite protéine comprend 118 amino-acides, son poids moleculaire est de l'ordre de 13 300 Daltons et son point isoélectrique est de l'ordre de 8,6.
Les dérivés de ladite protéine sont modifiés au niveau de l'une des histidines par fixation d'un groupe alkyle de formule R-CH₂-X.
Application à la détection de cellules tumorales.
摘要翻译：从家庭眼镜蛇科的蛇的毒液中分离的碱性蛋白质，特别是从一个眼镜蛇蛇和更爱特别是从眼镜蛇黑颈鹤和/或眼镜蛇罗非鱼淡紫，指定磷脂酶A2（PLA2），片段和该蛋白的衍生物，处理用于获得它们和医药组合物，其在人类和/或兽用药物可用，以及诊断剂含有上述蛋白质和/或其衍生物和/或它的片段。 ... 上述的蛋白质包含118个氨基酸，其分子量为13,300道尔顿的数量级和其等电点为8.6的数量级。 ... 上述蛋白质的衍生物是通过结合于烷基的式R-CH 2 -X的基团在组氨酸的一个修改。 ... 在检测肿瘤细胞的使用。

77. 发明公开

EP0213391A2 Antigenic modification of polypeptides 失效
标题翻译： Antigenische Modifizierung von Peptiden。
公开(公告)号：EP0213391A2
公开(公告)日：1987-03-11
申请号：EP86110378.6
申请日：1983-05-20
申请人： The Ohio State University
发明人： Stevens, Vernon Cecil
IPC分类号： A61K39/00 , A61K39/385 , A61K37/38 , C07K17/00
CPC分类号： C07K1/1075 , A61K38/00 , A61K39/00 , A61K39/0006 , A61K39/385 , A61K2039/5555 , A61K2039/55555 , A61K2039/55566 , A61K2039/6012 , A61K2039/627 , A61K2039/64 , C07K1/1072 , C07K1/1077 , C07K14/59 , Y10S530/806 , Y10S930/11
摘要： Modified polypeptides capable of provoking the formation of antibodies in an animal may be produced by forming a linear polymer of polypeptide fragments, each having a molecular structure similar to a fragment of the protein to which antibodies are to be provoked. Such linear polymers can be made more immunogenic than the proteins to which they are related without introducing undesirable extraneous materials into the animal being treated, but have reproduc- able immunogenic properties. Protein which are not endogenous or immunogenic to an animal can be chemically modified so as to make them more immunogenic. Also, modified antigens useful for fertility control can be produced by chemical modification of zona pellucida or sperm antigens. These modified polypeptides. antigens and modified antigens are desirably administered in the form of a vaccine having a vehicle comprising a mixture of mannide monooleate with Squalene and/or Squalene.
摘要翻译：能够诱发动物形成抗体的修饰的多肽可以通过形成多肽片段的线性聚合物来生产，每个片段具有类似于要引发抗体的蛋白质片段的分子结构。这样的线性聚合物可以比它们相关的蛋白质更具免疫原性，而不会将不需要的外来材料引入被治疗的动物中，但具有可再现的免疫原性。对动物不是内源性或免疫原性的蛋白质可以进行化学修饰，以使其更具免疫原性。此外，可以通过化学修饰透明带或精子抗原来产生用于生育控制的修饰抗原。这些修饰的多肽，抗原和修饰的抗原理想地以具有载体的疫苗形式施用，所述载体包含甘露苷单油酸酯与角鲨烯和/或角鲨烯的混合物。

78. 发明授权

EP0000938B1 Macromolecular covalent conjugates, methods for preparing and pharmaceutical compositions containing them 失效
标题翻译：大分子共价偶联物，其制备方法和含有它们的药物组合物
公开(公告)号：EP0000938B1
公开(公告)日：1984-10-17
申请号：EP78100717.4
申请日：1978-08-21
申请人： NATIONAL RESEARCH DEVELOPMENT CORPORATION
发明人： Ross, Walter Charles Joseph
IPC分类号： C07G7/00 , A61K37/02 , A61K39/00
CPC分类号： G01N33/531 , A61K39/00 , A61K47/6889 , C07K1/1072 , C12N11/06 , Y10S530/825 , Y10S530/866

79. 发明授权

EP2726494B1 USING SORTASES TO INSTALL CLICK CHEMISTRY HANDLES FOR PROTEIN LIGATION 有权转让
标题翻译： VERWENDUNG VON SORTASEN ZUR安装VON CLICK-CHEMIE-HANDLESFÜRPROTEINLIGATION
公开(公告)号：EP2726494B1
公开(公告)日：2017-01-04
申请号：EP12804570.5
申请日：2012-06-28
申请人： Whitehead Institute For Biomedical Research
发明人： PLOEGH, Hidde, L. , WITTE, Martin, D. , YODER, Nicholas, C.
IPC分类号： C07K1/06 , C12P21/04 , C07K16/46 , C07K19/00 , C12N9/00 , C12N9/10 , C12N15/62 , C12P21/00
CPC分类号： C07K16/468 , C07K1/107 , C07K1/1072 , C07K16/18 , C07K2317/31 , C07K2317/569 , C07K2317/62 , C07K2319/00 , C07K2319/21 , C12N9/52 , C12N15/62

80. 发明授权

EP2548967B1 Aldehyde tags, uses thereof in site-specific protein modification 有权
标题翻译：醛标记及其在特定位点的蛋白质修饰的应用
公开(公告)号：EP2548967B1
公开(公告)日：2016-12-28
申请号：EP12176853.5
申请日：2007-09-19
申请人： The Regents of the University of California
发明人： Carrico, Isaac S. , Carlson, Brian L. , Wu, Peng , Bertozzi, Carolyn
IPC分类号： C12P21/06 , C07K1/00
CPC分类号： C07K16/00 , A61K38/1825 , A61K38/215 , A61K38/37 , A61K38/45 , A61K39/00 , C07K1/006 , C07K1/1072 , C07K1/13 , C07K14/00 , C07K14/35 , C07K14/565 , C07K16/46 , C07K2317/24 , C07K2317/40 , C07K2317/52 , C07K2317/622 , C12N9/13 , C12N9/96 , C12P21/00 , C12P21/005 , C12Y208/02
摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.

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