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    • 71. 发明公开
    • Variants of glycoside hydrolases
    • 糖苷水解酶的变体
    • EP2617825A1
    • 2013-07-24
    • EP12194265.0
    • 2004-08-25
    • Novozymes, Inc.
    • Teter, SarahCherry, JoelWard, ConnieHarris, PaulJones, AubreyYi, Jung
    • C12N9/42C12N15/56C12P19/14
    • C12N9/2437C11D3/38645C12N9/2434C12N9/2445C12P7/10C12P19/02C12P19/14C12Y302/01021C12Y302/01091C12Y302/01176
    • The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
    • 本发明涉及母体糖苷水解酶的变体,其在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467和 486的SEQ ID NO:2的氨基酸1至513,并且任选地在对应于SEQ ID NO:2的8,22,41,59,57,113,193,196,226,227,246, 在对应于SEQ ID NO:2的8,22,41,49,57,113,113,115,117,118,117,117,117,117,117,117,119,129,129,129,112,131,112,212,212,212, 其中所述变体具有糖苷水解酶活性,所述变体具有糖苷水解酶活性,所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含该核苷酸序列的核酸构建体,载体和宿主细胞。