专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明公开

EP2976424A1 STABILISATION OF BIOLOGICAL SAMPLES 有权
标题翻译：镇定生物样品
公开(公告)号：EP2976424A1
公开(公告)日：2016-01-27
申请号：EP14714588.2
申请日：2014-03-18
申请人： Qiagen GmbH
发明人： WYRICH, Ralf , VOSS, Thorsten , GÜNTHER, Kalle , OELMÜLLER, Uwe
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1003 , C12Q1/6841 , C12Q2527/125 , C12Q2527/127 , C12Q2527/156
摘要： The present invention provides methods and composition suitable for stabilizing cell-containing samples such as blood samples. The stabilizers used are primary or secondary carboxylic acid amides.

52. 发明公开

EP2847330A4 BUFFER FOR ONE-STEP DNA EXTRACTION 有权
标题翻译：在EINEM SCHRITT中的PUFFER ZUR DNS-EXTRAKTION
公开(公告)号：EP2847330A4
公开(公告)日：2016-01-06
申请号：EP13787594
申请日：2013-05-08
申请人： BIO RAD LABORATORIES
发明人： RUEGG BRYONY , MILLER INGRID
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C07H21/04
摘要： DNA is extracted from epithelial cells and other cells without cell walls, by use of a DNA extraction buffer that contains a C1-C4 alcohol, a cell lysis detergent, and a buffering agent maintained at a slightly basic pH. The cells are immersed in the buffer and gently agitated at ambient temperature, and DNA extraction and precipitation occur in thirty minutes or less, and often in five minutes or less.
摘要翻译：通过使用含有C1-C4醇的DNA提取缓冲液，细胞裂解洗涤剂和维持在稍微碱性pH的缓冲剂，从上皮细胞和没有细胞壁的其它细胞提取DNA。将细胞浸入缓冲液中并在环境温度下轻轻搅拌，DNA提取和沉淀发生在30分钟以内，通常在5分钟以内。

53. 发明公开

EP2954054A1 METHOD FOR SEPARATING DNA BY SIZE 有权
标题翻译： VERFAHREN ZUM TRENNEN VON DNA ANHAND DERGRÖSSE
公开(公告)号：EP2954054A1
公开(公告)日：2015-12-16
申请号：EP14705735.0
申请日：2014-02-07
申请人： Qiagen GmbH
发明人： SPERLING, Tanya , SCHOLLE, Nicola , SINGER, Thorsten , HIESINGER, Margit
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q1/6869 , C12Q2525/204 , C12Q2527/125
摘要： The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.
摘要翻译：本发明提供了从含有DNA的样品中分离具有高于某一截止值的DNA分子的方法，其包括a）使样品与包含离液剂和缓冲剂的结合缓冲液接触以提供结合混合物，将具有大于截止值的大小的DNA分子与具有含硅表面的结合基质结合，其中所述截止值由所述结合混合物的pH值确定; b）将结合的DNA与剩余的样品分离; c）任选地洗涤结合的DNA; 和d）任选地从结合基质中洗脱结合的DNA。所述方法允许DNA分子的大小选择性纯化。

54. 发明公开

EP2940136A1 Method for isolating poly(A) nucleic acids 审中-公开
标题翻译： Verfahren zur Isolierung von Poly（A）-Nukleinsäuren
公开(公告)号：EP2940136A1
公开(公告)日：2015-11-04
申请号：EP14166712.1
申请日：2014-04-30
申请人： QIAGEN GmbH
发明人： Christoffel, Gabriele , Schlumpberger, Martin , O'Neil, Dominic
IPC分类号： C12N15/09
CPC分类号： C12N15/1013 , C12N15/1003 , C12Q2565/519
摘要： The present invention pertains inter alia to a method for isolating poly(A) nucleic acids having a single stranded poly(A) stretch from a nucleic acid containing sample comprising:
(a) providing a hybridization composition comprising:
i) a nucleic acid containing sample;
ii) a hybridization solution comprising:
aa. a sodium salt;
bb. a quaternary ammonium salt;
wherein the components of the hybridization solution can be added as single solution to the sample or may be added separately in any order
to the sample;

iii) a capture probe capable of hybridizing to the poly(A) stretch of the poly(A) nucleic acids;

and incubating said hybridization composition under conditions to form nucleic acid-hybrids between the poly(A) nucleic acids and the capture probe;
(b) separating the formed hybrids from the remaining sample.
The method is in particular suitable for efficiently isolating poly(A) RNA from various samples while avoiding carry-over of unwanted non-poly(A) nucleic acids such as rRNA. Also provided are advantageous further methods, hybridization solutions and kits.
摘要翻译：本发明特别涉及从含有核酸的样品中分离具有单链聚（A）区段的聚（A）核酸的方法，所述方法包括：（a）提供杂交组合物，其包含：i）含有核酸的样品 ; ii）杂交溶液，其包含：aa。钠盐; BB。季铵盐; 其中杂交溶液的组分可以作为单一溶液加入到样品中，或者可以以任何顺序分别加入到样品中; iii）能够与所述聚（A）核酸的聚（A）段杂交的捕获探针; 并在条件下孵育所述杂交组合物以在聚（A）核酸和捕获探针之间形成核酸杂交体; （b）将形成的杂交体与剩余的样品分离。该方法特别适合于有效地从各种样品中分离聚（A）RNA，同时避免不需要的非聚（A）核酸例如rRNA的携带。还提供了有利的另外的方法，杂交溶液和试剂盒。

55. 发明授权

EP2614147B1 METHOD OF ISOLATING PURIFIED RNA WITH REDUCED DNA CONTAMINATIONS 有权
标题翻译：方法纯化RNA具有减少污染DNS ISOLATION
公开(公告)号：EP2614147B1
公开(公告)日：2015-11-04
申请号：EP11752549.3
申请日：2011-09-06
申请人： Qiagen GmbH
发明人： CHRISTOFFEL, Gabriele
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C07H1/08 , C07H1/06 , C07H19/04 , C07H19/06 , C07H19/067 , C07H19/073 , C07H19/16 , C07H19/167 , C12N15/1003

56. 发明公开

EP2776587A4 CEREBROSPINAL FLUID ASSAY 审中-公开
标题翻译： LIQUOR测定法
公开(公告)号：EP2776587A4
公开(公告)日：2015-07-15
申请号：EP12847048
申请日：2012-11-12
申请人： EXOSOME DIAGNOSTICS INC
发明人： SKOG JOHAN KARL OLOV
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6806 , C12N15/1003 , C12N15/1017 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , C12Q2600/178
摘要： The present invention is directed to methods of isolating particles, such as nucleic acid-containing particles or microvesicles, from a biological sample and extracting nucleic acids therefrom, wherein the biological sample is cerebrospinal fluid. The present invention further provides methods for aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker associated with a disease or medical condition thereof.

57. 发明公开

EP2890980A1 A METHOD FOR OBTAINING BLOOD PLASMA FROM A WHOLE BLOOD SAMPLE 有权
标题翻译：一种获得血浆从全血样本
公开(公告)号：EP2890980A1
公开(公告)日：2015-07-08
申请号：EP13759156.6
申请日：2013-08-29
申请人： Qiagen GmbH
发明人： WENDE, Andy , WERNER, Sabine , HIMMELREICH, Ralf
IPC分类号： G01N33/49 , G01N33/50
CPC分类号： G01N33/491 , C12N15/1003 , C12Q1/6806
摘要： A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma.

58. 发明公开

EP2888363A1 METHOD FOR ISOLATING NUCLEIC ACIDS FROM A FORMALDEHYDE RELEASER STABILIZED SAMPLE 有权
标题翻译：方法分离核酸从具有FORMALDEHYDRELEASER稳定型探头
公开(公告)号：EP2888363A1
公开(公告)日：2015-07-01
申请号：EP13756018.1
申请日：2013-08-21
申请人： Qiagen GmbH
发明人： GÜNTHER, Kalle , WYRICH, Ralf , OELMÜLLER, Uwe
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要： The present invention pertains to a method for isolation and purification of nucleic acids from a stabilized sample or portion or fraction thereof, wherein the sample stabilization involved the use of at least one formaldehyde releaser and wherein the isolation of the nucleic acids from the stabilized sample or portion or fraction thereof involves the use of at least one cationic detergent during lysis.
摘要翻译：本发明涉及一种用于从一个稳定化的样品或其部分或其级分的分离和纯化核酸的方法，worin样品稳定化涉及使用至少一种甲醛释放剂的，并从稳定化的样品worin的核酸的分离或部或其馏分涉及到裂解过程中使用至少一种阳离子洗涤剂。

59. 发明授权

EP2557161B1 Verfahren zur Isolierung von RNA aus Vollblutproben 有权
标题翻译：一种用于RNA的从全血样品中分离过程
公开(公告)号：EP2557161B1
公开(公告)日：2015-05-20
申请号：EP12171498.4
申请日：2012-06-11
申请人： AXAGARIUS GmbH & Co. KG
发明人： Kirsch, Christoph, Dr. , Bayard, Claudia , Meusel, Markus, Dr. , Zinn, Thomas, Dr. , Wagner, Carolin , Möller, Klaus, Dr.
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12N1/06 , C12N15/1003

60. 发明公开

EP2670868A4 METHODS FOR ENRICHING MICROPARTICLES OR NUCLEIC ACIDS USING BINDING MOLECULES 审中-公开
标题翻译：工艺微颗粒或核酸通过结合分子富集
公开(公告)号：EP2670868A4
公开(公告)日：2015-05-06
申请号：EP12742603
申请日：2012-01-31
申请人： ESOTERIX GENETIC LAB LLC
发明人： OKAMOTO PATRICIA , GODOSKI JAN , SCHOLL THOMAS
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1006 , C12N15/1003

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式