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    • 60. 发明公开
    • A method of fluid bed chromatography
    • 流化床色谱法
    • EP0978311A1
    • 2000-02-09
    • EP99105046.9
    • 1991-07-08
    • UPFRONT CHROMATOGRAPHY A/S
    • Lihme, Allan Otto FogNielsen, Claus SchäferBog-Hansen, Thorkild Christian
    • B01J8/20B01D15/02B01D15/08
    • B01J20/28019B01D15/1807B01D2215/021B01J2/16B01J8/008B01J8/085B01J8/1845B01J8/1872B01J8/1881B01J8/20B01J8/22B01J8/32B01J13/02B01J20/28004B01J20/28011B01J20/28014B01J20/28021B01J20/28026B01J20/2803B01J20/3219B01J20/3251B01J20/3253B01J20/3255B01J20/3274B01J47/10C02F1/286C02F1/722C02F3/085C02F3/10C02F3/108C02F3/342Y02W10/15Y10S435/815Y10S530/811Y10S530/812Y10S530/813
    • A method of fluid bed chromatography in a liquid wherein molecules in the liquid are bound to at least one active substance which is chemically bound to fluid bed particles having pores allowing access of the molecules to the interior thereof, wherein the fluid bed particles consist of: (i) a single density controlling particle which is either (a) a hollow low density controlling particle which is impermeable to the liquid and has a density providing floatation of the fluid bed particle in the liquid, or (b) a high density controlling particle having a density providing sedimentation of the fluid bed particle in the liquid; and (ii) a matrix formed by consolidating at least one conglomerating agent selected from the group consisting of natural and synthetic organic monomers and polymers, said matrix having said single density controlling particle; and said matrix having the active substance for binding the molecules in the liquid covalently bound thereto; and wherein the fluid bed particles exhibit (i) a relative density with respect to the liquid which is less than 0.95 or greater than 1.1, and (ii) a particle size in the range of 1-1000 µm; said relative density and said particle size range of said fluid bed particles being selected to provide desired floatation/sedimentation properties of the fluid bed particles in the liquid; and to provide substantially no turbulence in the fluid bed.
    • 液体中的流化床色谱法,其中液体中的分子与至少一种活性物质结合,所述活性物质化学结合到流化床颗粒上,所述流化床颗粒具有允许分子进入其内部的孔,其中所述流化床颗粒由以下物质组成: (i)单一的密度控制颗粒,其是(a)中空的低密度控制颗粒,其是液体不可渗透的并具有提供流化床颗粒在液体中漂浮的密度,或(b)高密度控制颗粒 具有使液体中的流化床颗粒沉降的密度; 和(ii)通过固结选自天然和合成有机单体和聚合物的至少一种凝聚剂而形成的基质,所述基质具有所述单一密度控制颗粒; 并且所述基质具有用于结合与其共价结合的液体中的分子的活性物质; 并且其中所述流化床颗粒表现出(i)相对于所述液体的相对密度小于0.95或大于1.1,和(ii)粒度在1-1000μm的范围内; 所述流化床颗粒的所述相对密度和所述颗粒大小范围被选择为提供所述流化床颗粒在所述液体中的期望的漂浮/沉降性质; 并在流化床中提供基本无紊流。