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41. 发明授权

EP0531372B1 A CELLULASE PREPARATION COMPRISING AN ENDOGLUCANASE ENZYME 失效
标题翻译： AN AN含酶内切葡聚糖酶纤维素酶。
公开(公告)号：EP0531372B1
公开(公告)日：1995-02-15
申请号：EP91909971.3
申请日：1991-05-08
申请人： NOVO NORDISK A/S
发明人： RASMUSSEN, Grethe , MIKKELSEN, Jan, Möller , SCHÜLEIN, Martin , PATKAR, Shamkant, Anant , HAGEN, Fred , HJORT, Carsten, Mailand , HASTRUP, Sven
IPC分类号： C12N9/42 , C12N15/56 , C11D3/386
CPC分类号： C12Q1/6895 , C11D3/38645 , C12N9/2437 , C12N15/80 , C12Y302/01004 , C12Y302/01039 , C12Y302/01091 , D06M16/003 , D06P5/137 , D06P5/158 , D21H17/005 , D21H21/10
摘要： The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.

42. 发明公开

EP0462065A3 Neue Signalsequenzen 失效
标题翻译：新信号序列
公开(公告)号：EP0462065A3
公开(公告)日：1992-05-20
申请号：EP91810430.8
申请日：1991-06-06
申请人： CIBA-GEIGY AG
发明人： Boller, Thomas, Prof.Dr. , Neuhaus, Jean-Marc, Dr. , Ryals, John, Dr.
IPC分类号： C12N15/62 , C12N15/56 , C12N15/29 , C07K7/08 , C07K7/10 , C12N5/10 , C12N1/21 , A01H5/00
CPC分类号： C07K7/08 , A01K2217/05 , C07K14/415 , C07K2319/00 , C12N9/244 , C12N9/2442 , C12N15/625 , C12N15/8221 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058 , C12Y302/01073
摘要： Die vorliegende Erfindung betrifft neue Peptidfragmente ['Targeting'-Signal], die aus der C-terminalen region [C-terminale Extension] pflanzlicher Vakuolenproteine erhältlich sind und die in operabler Verknüpfung mit einem beliebigen Proteinmolekül für eine gezielte Einschleusung der mit diesen Peptidfragmenten assoziierten Proteine in die pflanzliche Vakuole sorgen sowie die besagten Peptidfragemente kodierende DNA-oleküle. Ein weiterer Gegenstand der vorliegenden Erfindung betrifft rekombinante DNA Moleküle, welche die erfindungsgemässe DNA Sequenz in operabler Verknüpfung mit einer exprimierbaren DNA enthalten sowie die davon abgeleiteten Vektoren. Ebenso umfasst sind Wirtszellen und/oder Wirtsorganismen, einschliesslich transgener Pflanzen, die besagte rekombinante DNA bzw. die davon abgeleiteten Vektoren enthalten.

43. 发明公开

EP0440304A1 Fungal resistant plants, process for obtaining fungal resistant plants and recombinant polynucleotides for use therein 失效
标题翻译：抗性真菌的植物，为了获得真菌抗性植物和为此使用，重组多核苷酸的过程。
公开(公告)号：EP0440304A1
公开(公告)日：1991-08-07
申请号：EP91200191.4
申请日：1991-01-30
申请人： MOGEN INTERNATIONAL N.V. , Rijksuniversiteit te Leiden
发明人： Cornelissen, Bernardus Johannes Clemens , Melchers, Leo Sjoerd , Meulenhoff, Elisabeth Josine Sophie , Van Roekel, Jeroen Sebastiaan Charles , Sela-Buurlage, Marianne Beatrix , Vloemans, Alexandra Aleida , Woloshuk, Charles Peter , Bol, John Ferdinand , Linthorst, Hubertus Josephus Maria
IPC分类号： C12N15/56 , C12N15/82 , A01H5/00 , C12N1/21 , A01N65/00
CPC分类号： C12N15/8282 , A01N65/38 , C12N9/244 , C12N9/2442 , C12Y302/01006 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058
摘要： Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and β-1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a β-1,3-glucanase.
摘要翻译：工厂设有对致病性真菌改进的抗。他们在遗传上与一个或多个核苷酸基本包含编码植物几丁质酶和β-1,3-葡聚糖酶的一个或多个基因转化。优选的是水解酶。所述，爱特别优选的细胞内形式是其通过凭借编码酶。所述的基因的修饰的靶向的计划质外体空间的那些形式。特别优选的是植物参展编码几丁质酶的至少一种基因和编码β-1,3-葡聚糖酶一种基因的相对表达。

44. 发明公开

EP0418695A1 Regulatorische DNA-Sequenz 失效
标题翻译： Regulatorische DNA-Sequenz。
公开(公告)号：EP0418695A1
公开(公告)日：1991-03-27
申请号：EP90117389.8
申请日：1990-09-10
申请人： CIBA-GEIGY AG
发明人： Meins, Jr., Frederick, Prof. Dr. , Shinshi, Hideaki, Dr. , Neuhaus, Jean-Marc, Dr.
IPC分类号： C12N15/82 , C12N15/56 , C12N1/21 , C12N15/10 , A01H5/00 , C12Q1/68 , A01N65/00
CPC分类号： C12Y302/01039 , C12N9/244 , C12N9/2442 , C12N15/67 , C12N15/8216 , C12Y302/01006 , C12Y302/01014 , C12Y302/01058
摘要： Die vorliegende Erfindung betrifft eine neue, im wesentlichen reine, regulatorische DNA Sequenz, welche aus der 5′-Region eines pflanzlichen Chitinasegens erhältlich ist und in operabler Verknüpfung mit einer beliebigen exprimierbaren DNA zu stark erhöhten Expressionsraten in transformierten Pflanzen führt.
Ein weiterer Gegenstand der vorliegenden Erfindung betrifft rekombinante DNA Moleküle, welche die erfindungsgemässe DNA Sequenz in operabler Verknüpfung mit einer exprimierbaren DNA enthalten sowie die davon abgeleiteten Vektoren, desweiteren Wirtszellen und/oder Wirtsorganismen, einschliesslich transgener Pflanzen, die besagte rekombinante DNA bzw. die davon abgeleiteten Vektoren enthalten.
Ebenso umfasst von der vorliegenden Erfindung ist ein neues Chitinasegen, das aus Nicotiana tabacum L. c.v. Havana 425 Pflanzen erhältlich ist und das in seiner 5′-nicht-translatierten Region die erfindungsgemässe, regulatorische DNA Sequenz enthält.
摘要翻译：本发明涉及可以从植物几丁质酶基因的5'区域获得的基本上纯的调节性DNA序列，并且当与任何所需的可表达DNA可操作地连接时，导致转化植物中表达率大大提高。本发明还涉及重组DNA分子，其含有可操作地连接到可表达DNA的DNA序列和由其衍生的载体，以及宿主细胞和/或宿主生物（包括转基因植物），其含有所述重组DNA或由其衍生的载体。本发明同样包括几丁质酶基因，其可以从烟草（Nicotiana tabacum L.c.v.）获得。哈瓦那425株植物，其中5'非翻译区含有根据本发明的调节性DNA序列。

45. 发明授权

EP2444487B1 Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them 有权
标题翻译：纤维素分解酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：EP2444487B1
公开(公告)日：2018-04-18
申请号：EP11007471.3
申请日：2006-12-08
申请人： BP Corporation North America Inc.
发明人： Gray, Kevin A. , Zhao, Lishan , Cayouette, Michelle H.
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/248 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01021 , C12Y302/01023 , C12Y302/01039 , C12Y302/01055 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y10T428/249921
摘要： The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a ²-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, ²-xylosidase), arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

46. 发明授权

EP2069389B1 GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权转让
标题翻译：葡聚糖酶，编码及其制造方法NUCLEIC为她和USE
公开(公告)号：EP2069389B1
公开(公告)日：2014-10-08
申请号：EP07875173.2
申请日：2007-08-04
申请人： BP Corporation North America Inc.
发明人： STEER, Brian , HEALEY, Shaun , ESTEGHLALIAN, Alireza , MILES, Stacy, Marie , BARRETT, Kenneth , QUADT, Rene
IPC分类号： C07K14/00 , C07K17/00 , C12N9/24 , C12N15/56
CPC分类号： C12N9/2437 , A23K20/147 , A23L33/18 , C09K8/035 , C09K8/52 , C09K8/68 , C09K8/74 , C09K8/805 , C09K8/90 , C09K2208/24 , C12N9/2405 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01039 , E21B43/26 , G06F19/22 , Y02E50/17

47. 发明公开

EP2468855A1 -GLUCANASE AND XYLANASE PREPARATION METHOD USING WHEAT BRAN, AND LIQUID CULTURE MEDIUM 审中-公开
标题翻译：用于β-葡聚糖酶和木聚糖酶麦麸液体培养基THEREFOR的制备
公开(公告)号：EP2468855A1
公开(公告)日：2012-06-27
申请号：EP10809953.2
申请日：2010-08-17
申请人： Asahi Group Holdings, Ltd.
发明人： FUKUDA, Kazuro
IPC分类号： C12N9/24 , C07H3/02 , C12N1/14 , C12P19/14
CPC分类号： C12P19/14 , C12N1/14 , C12N1/22 , C12N9/2437 , C12N9/244 , C12N9/248 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01039
摘要： To produce cellulase having excellent ability to decompose cellulosic resources containing xylan at low cost.
A method for producing β-glucanase and xylanase, comprising the step of culturing a microorganism classified under the genus Trichoderma by using a liquid culture medium which contains (a) wheat bran as a carbon source and (b) ammonia nitrogen or amino nitrogen as a nitrogen source.
摘要翻译：为了生产出具有优异的纤维素分解低成本含木聚糖的纤维素资源的能力。一种用于生产²葡聚糖酶和木聚糖酶，其包括通过使用液体培养基其中含有（a）麦麸作为碳源和（b）氨态氮或氨基态氮作为培养属于木霉属的微生物的步骤方法氮源。

48. 发明公开

EP2444490A1 Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them 有权
标题翻译：纤维素降解酶，核酸编码这些，以及它们的制备和使用过程
公开(公告)号：EP2444490A1
公开(公告)日：2012-04-25
申请号：EP11007478.8
申请日：2006-12-08
申请人： Verenium Corporation
发明人： Gray, Kevin A. , Zhao, Lishan , Cayouette, Michelle H.
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/248 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01021 , C12Y302/01023 , C12Y302/01039 , C12Y302/01055 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y10T428/249921
摘要： The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase), arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
摘要翻译：本发明提供具有任何纤维素分解活性，例如纤维素酶活性，内切葡聚糖酶，纤维二糖水解，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，阿拉伯呋喃糖酶在一个²木糖苷多肽，和/或寡聚体酶活性，多核苷酸编码本文多肽，以及制备和使用这些多核苷酸和多肽的方法。在一个方面，本发明涉及具有任何纤维素分解活性，例如纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，²木糖苷酶），阿拉伯呋喃糖苷酶，以多肽和/或寡聚体酶活性，包括热电表和耐热活性，多核苷酸编码合成的酶，以及制备和使用这些多核苷酸和多肽。在一个方面，本发明提供具有对寡聚体酶活性的多肽，E. G.，酶没有转换顽抗可溶性寡聚体可发酵的糖在生物质的糖化。本发明的多肽可以在多种药物，农业，食品和饲料加工和工业环境中使用。因此本发明提供的组合物或包含酶包含本发明的至少一种酶的混合物制造的产品。

49. 发明公开

EP2444487A1 Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them 有权
标题翻译：纤维素分解酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：EP2444487A1
公开(公告)日：2012-04-25
申请号：EP11007471.3
申请日：2006-12-08
申请人： Verenium Corporation
发明人： Gray, Kevin A. , Zhao, Lishan , Cayouette, Michelle H.
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12N9/2402 , C12N9/2405 , C12N9/248 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01021 , C12Y302/01023 , C12Y302/01039 , C12Y302/01055 , C12Y302/01091 , Y02E50/16 , Y02E50/17 , Y10T428/249921
摘要： The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
摘要翻译：本发明提供了具有任何纤维素分解活性的多肽，例如纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，β-木糖苷酶，阿拉伯呋喃糖苷酶和/或寡聚体酶活性，编码这些的多核苷酸多肽，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有任何纤维素分解活性的多肽，例如纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，β-木糖苷酶，阿拉伯呋喃糖苷酶和/或寡聚体酶活性，包括热稳定的和耐热活性和编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。在一个方面，本发明提供了具有寡聚体酶活性的多肽，例如在生物质糖化中将难溶性可溶性寡聚物转化成可发酵糖的酶。本发明的多肽可以用于各种制药，农业，食品和饲料加工和工业环境中。本发明还提供包含含有至少一种本发明酶的酶混合物的组合物或产品。

50. 发明公开

EP2444413A1 Methods for oil or gas well drilling, washing and/or fracturing 审中-公开
$Methods for oil or gas well drilling, washing and/or fracturing$
标题翻译：瓦尔 - 博洪根，弗洛伊德和“压裂”
公开(公告)号：EP2444413A1
公开(公告)日：2012-04-25
申请号：EP12150998.8
申请日：2007-08-04
申请人： Verenium Corporation
发明人： Steer, Brian , Healey, Shaun , Esteghlalian, Alireza , Miles, Stacy Marie , Barrett, Kenneth , Quadt, Rene
IPC分类号： C07K14/00 , C07K17/00 , C12N9/24
CPC分类号： C12N9/2437 , A23K20/147 , A23L33/18 , C09K8/035 , C09K8/52 , C09K8/68 , C09K8/74 , C09K8/805 , C09K8/90 , C09K2208/24 , C12N9/2405 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01039 , E21B43/26 , G06F19/22 , Y02E50/17
摘要： A drilling or oil and gas well clean up and/or fracturing method comprises providing at least one polymer-degrading enzyme comprising a polypeptide of SEQ ID NO:15, which is added to a drilling fluid used during an oil and gas well drilling operation, or to a fluid used for an oil and gas well clean up and/or fracturing operation. Combined with a second enzyme comprising a polypeptide of SEQ ID NO: 2, the enzyme comprising a polypeptide of SEQ ID NO 15 can be used to degrade a xanthan, a guar, a hydroxyalkyl guar, a carboxyalkyl guar, a guar gum, a guar gum powder, a lignified coat of guar seeds or a solidified guar gum.
摘要翻译：钻井或油气净化和/或压裂方法包括提供至少一种包含SEQ ID NO：15的多肽的聚合物降解酶，其被添加到在油气井钻井操作期间使用的钻井液中，或用于油气清洁和/或压裂操作的流体。与包含SEQ ID NO：2的多肽的第二种酶组合，包含SEQ ID NO 15的多肽的酶可用于降解黄原胶，瓜尔胶，羟烷基瓜尔胶，羧烷基瓜尔胶，瓜尔胶，瓜尔胶胶粉，木瓜化的瓜尔胶种皮或固化的瓜尔胶。

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