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41. 发明公开

EP0831923A1 SEPARATION OF ACTIVE COMPLEXES 失效
标题翻译：分离活性复合物
公开(公告)号：EP0831923A1
公开(公告)日：1998-04-01
申请号：EP96917839.0
申请日：1996-05-28
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： SKOZA, Francis, C., Jr. , XU, Yuhong , WANG, Jinkang
IPC分类号： C12N15 , A61K9 , A61K47 , A61K48 , C07D401 , C07F9 , C07K14 , C08G83 , C12Q1
CPC分类号： A61K9/1271 , A61K9/1272 , A61K31/70 , A61K47/54 , A61K47/543 , A61K47/55 , A61K47/645 , A61K47/6911 , A61K47/6929 , A61K48/00 , B82Y5/00 , C07D401/14 , C07F9/10 , C07K14/005 , C08G83/003 , C12N15/87 , C12N15/88 , C12N2710/22022 , Y10S977/797 , Y10S977/907
摘要： The invention separates defined, active complexes by a characteristic from defined, active complexes that share a particular physicochemical characteristic such as density, surface charge or particle size are separated from complexes fromed by the association of a polynucleotide with a transfecting component that increases transfection activity, such as a lipid, cationic lipid, liposome, peptide, cationic peptide, dendrimer or polycation. In a preferred embodiment, polynucleotide-transfecting component complexes are ultracentrifuged to resolve one or more bands corresponding to complexes having a specific polynucleotide-transfecting component interaction. Polynucleotide complexes having a cationic liposome transfecting component resolve into two primary bands corresponding to complexes formed either under excess lipid conditions or under excess polynucleotide conditions. In an alternate embodiment, polynucleotide-transfecting component complexes are resolved using cross-flow electrophoresis to identify complexes having specific interactions and to separate them from excess initial components.

42. 发明公开

EP0805209A2 Nukleinsäurekonstrukte mit Genen kodierend für Transportsignale 失效
标题翻译： Nukleinsäurekonstruktemit Genen kodierendfürTransportsignale
公开(公告)号：EP0805209A2
公开(公告)日：1997-11-05
申请号：EP97106531.3
申请日：1997-04-21
申请人： HOECHST AKTIENGESELLSCHAFT
发明人： Sedlacek, Hans-Harald, Prof. Dr. , Müller, Rolf, Prof. Dr. , Lührmann, Reinhard, Prof. Dr.
IPC分类号： C12N15/86 , C12N15/62
CPC分类号： C12N15/86 , C12N15/63 , C12N15/85 , C12N2710/16622 , C12N2710/22022 , C12N2740/16322 , C12N2830/007 , C12N2830/008 , C12N2830/15 , C12N2840/20
摘要： Offenbart werden Nukleinsäurekonstrukte, die ein nukleäres Retentions-Signal aufweisen, das in Leserichtung stromabwärts mit einem Transgen verknüpft ist. Durch das nukleäre Retentions-Signal kann die Anwesenheit des Transkriptionsproduktes im Zellkern oder auch der intrazelluläre Transport des Transkriptionsproduktes gesteuert werden.
摘要翻译：包含与转基因3'端连接的核保留信号（NRS）序列的核酸构建体是新的。还要求保护的是含有这种构建体的分离的细胞。

43. 发明公开

EP0352894A3 Production of cosmetic or pharmaceutical composition 失效
标题翻译：生产化妆品或药物组合物
公开(公告)号：EP0352894A3
公开(公告)日：1990-07-11
申请号：EP89306057.4
申请日：1989-06-15
申请人： UNILEVER PLC , UNILEVER N.V.
发明人： Bayley, Susan Ann
IPC分类号： C12N5/00 , C12N15/00 , C12P21/00 , A61K7/00
CPC分类号： C12N15/86 , A61K8/4953 , A61K8/99 , A61K2800/86 , A61Q7/00 , C07K14/005 , C12N5/0627 , C12N2510/04 , C12N2710/22022 , C12N2740/13043
摘要： Dermal papillae cells are immortalized and cultured to produce hair growth promoter. The hair growth promoter is then collected and used to prepare compositions for application to the human skin.

44. 发明公开

EP0352894A2 Production of cosmetic or pharmaceutical composition 失效
标题翻译： Herstellung einer kosmetischen oder pharmazeutischen Zusammensetzung。
公开(公告)号：EP0352894A2
公开(公告)日：1990-01-31
申请号：EP89306057.4
申请日：1989-06-15
申请人： UNILEVER PLC , UNILEVER N.V.
发明人： Bayley, Susan Ann
IPC分类号： C12N5/00 , C12N15/00 , C12P21/00 , A61K7/00
CPC分类号： C12N15/86 , A61K8/4953 , A61K8/99 , A61K2800/86 , A61Q7/00 , C07K14/005 , C12N5/0627 , C12N2510/04 , C12N2710/22022 , C12N2740/13043
摘要： Dermal papillae cells are immortalized and cultured to produce hair growth promoter. The hair growth promoter is then collected and used to prepare compositions for application to the human skin.
摘要翻译：将真皮乳头状细胞永生化并培养以产生毛发生长促进剂。然后收集毛发生长促进剂并用于制备用于施用于人皮肤的组合物。

45. 发明公开

EP0041313A3 DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon-like polypeptides 无效
标题翻译： DNA序列，重组DNA分子和生产人类纤维蛋白样干扰素多肽的方法
公开(公告)号：EP0041313A3
公开(公告)日：1982-08-18
申请号：EP81301414
申请日：1981-04-01
申请人： BIOGEN N.V.
发明人： Fiers, Walter Charles, Dr.
IPC分类号： C12N15/00 , C12P21/02 , C07H21/04 , C07C103/52 , A61K45/02 , C12N01/20
CPC分类号： C07K14/005 , A61K38/00 , C07K14/565 , C07K2319/00 , C07K2319/02 , C07K2319/40 , C07K2319/75 , C12N15/52 , C12N15/62 , C12N15/73 , C12N2710/22022
摘要： A DNA sequence consisting of the DNA inserts of G-pBR322(Pst)/HFIF1, G-pBR322(Pst)/HFIF3, G-pBR322 (Pst)/HFIF-6, G-pBR322(Pst)/HFIF7, DNA sequences which hybridize to any of the foregoing DNA inserts and DNA sequences, from whatever source obtained, including natural, synthetic or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions to any of the foregoing DNA sequences or inserts.
摘要翻译：由G-pBR322（Pst）/ HFIF1，G-pBR322（Pst）/ HFIF3，G-pBR322（Pst）/ HFIF-6，G-pBR322（Pst）/ HFIF7）的DNA插入物组成的DNA序列，与任何前述DNA插入片段和DNA序列杂交，从任何获得的来源，包括天然的，合成的或半合成的来源，通过突变相关，包括单个或多个碱基取代，缺失，插入和倒转到任何前述DNA 序列或插入。

46. 发明公开

EP0041313A2 DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon 无效
标题翻译： DNA序列的重组DNA分子和用于生产人成纤维细胞干扰素的过程。
公开(公告)号：EP0041313A2
公开(公告)日：1981-12-09
申请号：EP81301414.9
申请日：1981-04-01
申请人： BIOGEN, INC.
发明人： Fiers, Walter Charles, Dr.
IPC分类号： C12N15/22 , C12P21/02 , C07H21/04 , A61K37/66
CPC分类号： C07K14/005 , A61K38/00 , C07K14/565 , C07K2319/00 , C07K2319/02 , C07K2319/40 , C07K2319/75 , C12N15/52 , C12N15/62 , C12N15/73 , C12N2710/22022
摘要： A DNA sequence consisting of the DNA inserts of G-pBR322(Pst)/HFIF1, G-pBR322(Pst)/HFIF3, G-pBR322 (Pst)/HFIF-6, G-pBR322(Pst)/HFIF7, DNA sequences which hybridize to any of the foregoing DNA inserts and DNA sequences, from whatever source obtained, including natural, synthetic or semi-synthetic sources, related by mutation, including single or multiple, base substitutions, deletions, insertions and inversions to any of the foregoing DNA sequences or inserts.
摘要翻译：的DNA序列，由...组成G-的pBR322（PST）/ HFIF1，G-的pBR322（PST）/ HFIF3，G-的pBR322（PST）/ HFIF-6，G-的pBR322（PST）/ HFIF7，DNA序列的DNA插入的哪杂交到任何上述DNA插入物和DNA序列，来自任何来源获得，包括天然的，合成的或半合成来源，通过突变有关，包括单个或多个，碱基替换，缺失，插入和倒位，以任何上述的DNA的序列或插入。

47. 发明公开

EP3031821A1 polyomavirus VLPs with a fusion protein 审中-公开
标题翻译：病毒 - 维生素（VLP）aus多瘤病毒融合蛋白
公开(公告)号：EP3031821A1
公开(公告)日：2016-06-15
申请号：EP14196847.9
申请日：2014-12-08
申请人： Life Science Inkubator
发明人： Franken, Sebastian , Glassmann, Alexander , Temme, Nadine
IPC分类号： C07K14/005 , C12N7/04
CPC分类号： C07K14/005 , C07K2319/06 , C07K2319/70 , C12N7/00 , C12N7/04 , C12N15/113 , C12N2310/14 , C12N2320/32 , C12N2710/22022 , C12N2710/22023 , C12N2710/22042
摘要： The disclosure relates to a fusion protein comprising a VP1 binding protein and an exogenous peptide, wherein the exogenous peptide comprises a cargo-securing peptide (CSP) and/or an endosome translocating peptide (ETP) and to virus like particles (VLP) comprising the fusion protein for use as drug delivery system.
Also provided are polynucleotides encoding the fusion protein, suitable expression vectors, host cells, production methods for the fusion protein and the VLP comprising the fusion protein.
摘要翻译：本公开涉及包含VP1结合蛋白和外源肽的融合蛋白，其中所述外源肽包含货物确保肽（CSP）和/或内体易位肽（ETP）和包含病毒样颗粒（VLP）的病毒样颗粒（VLP）融合蛋白用作药物递送系统。还提供了编码融合蛋白的多核苷酸，合适的表达载体，宿主细胞，融合蛋白的产生方法和包含融合蛋白的VLP。

48. 发明公开

EP3031820A1 JC Polyomavirus VLP (virus-like particle) with a targeting peptide 审中-公开
标题翻译： JC多瘤病毒病毒 - 维生素（VLP）靶向肽
公开(公告)号：EP3031820A1
公开(公告)日：2016-06-15
申请号：EP14196845.3
申请日：2014-12-08
申请人： Life Science Inkubator
发明人： Franken, Sebastian , Glassmann, Alexander , Temme, Nadine
IPC分类号： C07K14/005 , C07K14/01 , C12N7/04
CPC分类号： C07K14/01 , C07K2/00 , C07K14/005 , C07K2319/01 , C12N7/04 , C12N2710/22022 , C12N2710/22023 , C12N2710/22042 , C12N2810/40
摘要： The disclosure relates to a fusion protein comprising at least a first and a second peptide, wherein
- the second peptide comprises a targeting region and a first and a second interaction region,
- the second peptide is located on the surface of the fusion protein;
- the second peptide comprises at least two interaction pairs, wherein an interaction pair is formed by an amino acid of the first interaction region and an amino acid of the second interaction region,
- the interaction between the amino acids of an interaction pair is covalent or noncovalent; and
- at least one interaction pair is a covalent interaction pair in which the amino acids are covalently bound,

and to virus like particles (VLP) comprising the fusion protein for use as drug delivery system.
Also provided are polynucleotides encoding the fusion protein, suitable expression vectors, host cells, production methods for the fusion protein and the VLP comprising the fusion protein.
摘要翻译：本公开涉及包含至少第一和第二肽的融合蛋白，其中 - 所述第二肽包含靶向区和第一和第二相互作用区，所述第二肽位于所述融合蛋白的表面上; - 第二肽包含至少两个相互作用对，其中相互作用对由第一相互作用区域的氨基酸和第二相互作用区域的氨基酸形成， - 相互作用对的氨基酸之间的相互作用是共价的或非共价键; 和 - 至少一个相互作用对是氨基酸共价结合的共价相互作用对，以及包含用作药物递送系统的融合蛋白的病毒样颗粒（VLP）。还提供了编码融合蛋白的多核苷酸，合适的表达载体，宿主细胞，融合蛋白的产生方法和包含融合蛋白的VLP。

49. 发明公开

EP2836598A1 GENE EXPRESSSION FROM MITOTICALLY STABLE EPISOMES 审中-公开
标题翻译：基因XPRESS SION从稳定有丝分裂的游离体
公开(公告)号：EP2836598A1
公开(公告)日：2015-02-18
申请号：EP13713483.9
申请日：2013-03-27
申请人： Royal Holloway And Bedford New College
发明人： DICKSON, George , YANEZ-MUNOS, Rafael , KYMALAINEN, Hanna
IPC分类号： C12N15/67 , C12N15/867
CPC分类号： C12N15/67 , C12N15/79 , C12N15/86 , C12N2710/22022 , C12N2740/16043 , C12N2800/108 , C12N2820/60 , C12N2830/46
摘要： The invention provides a method of expressing a transgene in a host cell. The method comprises exposing a host cell, which comprises a genetic construct comprising a transgene, to conditions which substantially block mitosis in the host cell, such that the construct forms a mitotically stable episome resulting in expression of the transgene. The invention extends to the use of such constructs for expressing transgenes in a range of different medical and heterologous protein production applications.

50. 发明公开

EP2785731A1 VECTORS AND HOST CELLS COMPRISING A MODIFIED SV40 PROMOTER FOR PROTEIN EXPRESSION 无效转让
标题翻译： VEKTOREN UND WIRTSZELLEN MIT EINEM MODIFIZIERTEN SV40-PROMOTER ZUR PROTEINEXPRESSION
公开(公告)号：EP2785731A1
公开(公告)日：2014-10-08
申请号：EP11815647.0
申请日：2011-11-30
申请人： AbbVie Biotherapeutics Inc.
发明人： HARTMAN, Taymar , TSO, Yun J. , HE, Yimin
IPC分类号： C07K14/025 , C12N15/85 , C07K16/00
CPC分类号： C12P21/00 , C07K14/005 , C07K16/00 , C07K16/2896 , C07K2317/14 , C07K2317/51 , C07K2317/515 , C12N7/00 , C12N15/85 , C12N2710/22022 , C12N2710/22043 , C12N2800/107 , C12N2800/24 , C12N2830/20 , C12N2830/60
摘要： The present disclosure is directed to expression vectors, comprising a weakened SV40 promoter, and recombinant mammalian cells capable of producing high levels of a polypeptide of interest, methods of generating and using such recombinant mammalian cells.
摘要翻译：本公开涉及包含弱化SV40启动子和能够产生高水平目标多肽的重组哺乳动物细胞的表达载体，产生和使用这些重组哺乳动物细胞的方法。

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