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    • 38. 发明公开
    • NEW CLICKABLE POLYMERS AND GELS FOR MICROARRAY AND OTHER APPLICATIONS
    • 用于微阵列和其他应用的新型可点击聚合物和凝胶
    • EP3230393A1
    • 2017-10-18
    • EP15832820.3
    • 2015-12-11
    • Chiari, Marcella
    • Chiari, Marcella
    • C09D133/26C08F220/40C08F220/54C08F220/56C08F230/08C09D143/04
    • C09D143/04C07H1/06C07H21/04C08F220/54C08L2203/02C08L2312/08C09D133/24C09D133/26G01N33/54353G01N33/545C08F2230/085C08F230/08
    • Fabrication of arrays, including glycan arrays, that combines the higher sensitivity of a layered Si—SiO2 substrate with novel immobilization chemistry via a “click” reaction. The novel immobilization approach allows the oriented attachment of glycans on a “clickable” polymeric coating. The surface equilibrium dissociation constant (KD) of Concanavalin A with eight synthetic glycans was determined using fluorescence microarray. The sensitivity provided by the novel microarray substrate enables the evaluation of the influence of the glycan surface density on surface KD values. The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. The click polymers can be prepared in different forms such as soluble polymers, hydrogels, and multi-layers. The polymers can be prepared directly by copolymerization or by copolymerization to form a pre-polymer which is then reacted to form the target polymer. Other uses include separations, including electrophoretic separations.
    • 阵列(包括聚糖阵列)的制作,通过“点击”反应将层状Si-SiO 2基底的更高灵敏度与新颖的固定化学组合在一起。 新型固定方法允许在“可点击”聚合物涂层上定向连接聚糖。 使用荧光微阵列测定伴刀豆球蛋白A与八种合成聚糖的表面平衡解离常数(K D)。 由新型微阵列基质提供的灵敏度能够评估聚糖表面密度对表面K D值的影响。 碳水化合物与多种生物靶标(包括抗体,蛋白质,病毒和细胞)的相互作用在生物学的许多方面都是至关重要的。 聚糖微阵列越来越多地用于确定聚糖结合蛋白的结合特异性。 点击聚合物可以以不同形式制备,例如可溶性聚合物,水凝胶和多层。 该聚合物可以通过共聚或共聚直接制备成预聚物,然后反应形成目标聚合物。 其他用途包括分离,包括电泳分离。