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31. 发明公开

EP0200425A2 Recombinant DNA expression vectors and DNA compounds 失效
标题翻译：重组DNA表达载体和DNA化合物
公开(公告)号：EP0200425A2
公开(公告)日：1986-12-10
申请号：EP86302873.4
申请日：1986-04-17
申请人： ELI LILLY AND COMPANY
发明人： Ingolia, Thomas Dominick , Queener, Stephen Wyatt , Samson, Suellen Mary , Skatrud, Paul Luther
IPC分类号： C12N15/80 , C12N9/00 , C12N15/69
摘要： The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA .has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C . acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase- encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
摘要翻译：本发明包含编码异青霉素N合成酶的新型DNA化合物，并且还包含相关方法，转化体和多肽。新颖的异青霉素N合成酶编码DNA及其相关的转录和翻译活化序列从顶头孢霉中分离并克隆到大肠杆菌克隆载体中。编码异青霉素N合成酶的DNA已用于构建新的大肠杆菌表达载体，其驱动大肠杆菌中稳定，活性和新颖的异青霉素N合成酶的表达。完整的顶头孢霉异青霉素N合成酶编码DNA和相关的转录和翻译活化序列也已被用于构建促进异青霉素N合成酶在C中表达的顶头孢霉表达载体。顶头孢菌。顶头孢霉转录和翻译活化序列还与潮霉素磷酸转移酶编码DNA片段融合并置于顶头孢霉表达载体上。还描述了新化合物和载体的有用衍生物。

32. 发明公开

EP0814163A3 Peptidoglycan biosynthetic gene murD from streptococcus pneumoniae 失效
标题翻译：肽聚糖生物合成产生MurD von肺炎链球菌
公开(公告)号：EP0814163A3
公开(公告)日：1999-10-27
申请号：EP97304281.5
申请日：1997-06-18
申请人： ELI LILLY AND COMPANY
发明人： Hoskins, Jo Ann , Peery, Robert Brown , Skatrud, Paul Luther , Wu, Chyun-Yeh Earnest
IPC分类号： C12N15/52 , C12N9/00 , C12Q1/25 , C12N1/21
CPC分类号： C12N9/93 , C07K2319/00
摘要： The invention provides isolated nucleic acid compounds encoding the murD stem peptide biosynthetic gene of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the MurD enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.

33. 发明公开

EP0875578A2 Penicllin binding protein 2A from staphylococcus aureus strain 27R and derivatives for use in purification therefor and assay for compounds effective against methicillin resistant organisms 失效
标题翻译：青霉素的衍生物从金黄色葡萄球菌菌株27R结合蛋白2A用于其纯化并测定耐甲氧西林的微生物有效化合物的
公开(公告)号：EP0875578A2
公开(公告)日：1998-11-04
申请号：EP98200530.8
申请日：1992-03-18
申请人： ELI LILLY AND COMPANY
发明人： Blaszczak, Larry Chris , Skatrud, Paul Luther , Smith, Michele Ceceil , Wu, Chyun-Yey Earnest
IPC分类号： C12N15/57 , C12N9/52 , C12N15/62 , C07K19/00
CPC分类号： C12N9/52 , C07K14/31 , C07K2319/00 , C07K2319/02 , C12N15/625
摘要： The invention provides a gene encoding a novel PBP2A penicillin binding protein isolated from the methicillin resistant S . aureus strain 27R. The invention further provides soluble forms of PBP2A proteins lacking the transmembrane association region yet retain their active site configuration of the PBP2A protein. The invention also provides a method of assaying for agents useful against methicillin resistant staphylococcus strains by creating a kinetically inert complex between a support-immobilized transition metal ion and a modified soluble form of the PBP2A protein incorporating a chelating agent. The invention further provides a cell free method of assaying for agents which bind the PBP2A-27R protein.
摘要翻译：本发明提供了编码来自耐甲氧西林金黄色葡萄球菌菌株27R分离的新颖的PBP2a青霉素结合蛋白的基因。本发明提供了缺乏跨膜区的关联，但仍保留了蛋白质的PBP2a的其活性位点结构的PBP2a蛋白的进一步可溶形式。因此本发明提供测定针对通过创建一个支撑固定化的过渡金属离子和蛋白质的PBP2a掺入的螯合剂修饰的可溶性形式之间的动力学惰性的复杂耐甲氧西林葡萄球菌菌株有用的试剂的方法。本发明还提供测定用于结合所述的PBP2a-27R蛋白质剂的无细胞方法。

34. 发明公开

EP0810286A2 Peptidoglycan biosynthetic gene murE from streptococcus pneumoniae 失效
标题翻译：肽多糖生物合成发炎型肺炎链球菌
公开(公告)号：EP0810286A2
公开(公告)日：1997-12-03
申请号：EP97303661.9
申请日：1997-05-29
申请人： ELI LILLY AND COMPANY
发明人： Peery, Robert Brown , Skatrud, Paul Luther
IPC分类号： C12N15/52 , C12N9/00 , C12Q1/25 , C12N1/21
CPC分类号： C12N9/93 , C07K2319/00
摘要： The invention provides isolated nucleic acid compounds encoding the murE stem peptide biosynthetic gene of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the murE enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.
摘要翻译：本发明提供了编码肺炎链球菌的小鼠胚胎干细胞生物合成基因的分离的核酸化合物。还提供了用于表达murE酶产物的载体和转化的异源宿主细胞，以及用于鉴定抑制干肽生物合成的化合物的方法。

35. 发明公开

EP0678578A3 Multiple drug resistance gene of Aureobasidium pullulans 失效
标题翻译：出芽短梗霉的多种药物Wiederstandsgen。
公开(公告)号：EP0678578A3
公开(公告)日：1996-12-04
申请号：EP95302542.6
申请日：1995-04-18
申请人： ELI LILLY AND COMPANY
发明人： Peery, Robert Brown , Skatrud, Paul Luther
IPC分类号： C12N15/31 , C07K14/37 , C12Q1/68 , G01N33/68 , C12N1/19
CPC分类号： C07K14/37
摘要： The invention provides isolated nucleic acid compounds encoding the multiple drug resistance protein of Aureobasidium pullulans . Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aureobasidium pullulans are also provided. The invention further provides assays which utilize these transformed host cells.

36. 发明授权

EP0281391B1 Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase 失效
标题翻译：重组DNA表达载体和DNA的组合物，其编码脱乙酸基C合成对于C合成酶和脱乙酰头孢菌素
公开(公告)号：EP0281391B1
公开(公告)日：1996-10-30
申请号：EP88301845.9
申请日：1988-03-03
申请人： ELI LILLY AND COMPANY
发明人： Ingolia, Thomas Dominick , Queener, Stephen Wyatt , Samson, Suellen Mary , Skatrud, Paul Luther
IPC分类号： C12N15/00 , C12N9/00 , C12P35/06
CPC分类号： C12N9/0071 , C12N15/52 , C12N15/70 , C12N15/80

37. 发明公开

EP0614976A3 Mammalian influx peptide transporter 失效
标题翻译：哺乳动物内流肽转运蛋白
公开(公告)号：EP0614976A3
公开(公告)日：1995-11-15
申请号：EP94300794.8
申请日：1994-02-03
申请人： ELI LILLY AND COMPANY
发明人： Dantzig, Anne Hollins , Hoskins, JoAnn , Skatrud, Paul Luther
IPC分类号： C12N15/12 , C12N15/85 , C12N5/10
CPC分类号： C07K14/47
摘要： The present invention provides isolated DNA compounds and recombinant DNA vectors that encode mammalian influx peptide transporter activity. The invention also provides host cells transformed with these vectors and a method for production of mammalian influx peptide transporter activity by recombinant DNA techniques. The invention also provides a method for identifying compounds that are transported into the cell by the influx peptide transporter.
摘要翻译：本发明提供了分离的DNA化合物和编码哺乳动物内流肽转运蛋白活性的重组DNA载体。本发明还提供了用这些载体转化的宿主细胞以及通过重组DNA技术产生哺乳动物内流肽转运蛋白活性的方法。本发明还提供了鉴定通过内流肽转运蛋白转运进入细胞的化合物的方法。

38. 发明公开

EP0625575A3 Fem A gene of staphylococcus epidermidis, fem A protein, and vectors of microorganisms comprising the fem A gene 失效
标题翻译： FEM从表皮葡萄球菌A基因，FEM的蛋白质和载体和含有该FEM的基因的微生物。
公开(公告)号：EP0625575A3
公开(公告)日：1995-02-22
申请号：EP94302950.4
申请日：1994-04-25
申请人： ELI LILLY AND COMPANY
发明人： Alborn, William Ernest, Jr. , Hoskins, Jo Ann , Skatrud, Paul Luther , Ünal, Serhat
IPC分类号： C12N15/31 , C12P21/02 , C07K13/00
CPC分类号： C07K14/31
摘要： The instant invention provides the femA gene of Staphylococcus epidermidis and all degenerate sequences thereof, the protein encoded by the femA gene (FemA), and vectors and microorganisms comprising genes encoding the FemA protein.

39. 发明公开

EP0624650A3 FemB gene of Staphylococcus epidermidis, FemB protein, and vectors and microorganisms comprising the FemB gen 失效
标题翻译：从表皮葡萄球菌，FEMB蛋白，和载体和含有该基因FEMB微生物FEMB基因。
公开(公告)号：EP0624650A3
公开(公告)日：1995-02-22
申请号：EP94302949.6
申请日：1994-04-25
申请人： ELI LILLY AND COMPANY
发明人： Alborn, Jr., William Ernest , Hoskins, Jo Ann , Skatrud, Paul Luther
IPC分类号： C12N15/31 , C12P21/02 , C07K13/00
CPC分类号： C07K14/31
摘要： The instant invention provides the femB gene of Staphylococcus epidermidis and all degenerate sequences thereof, the protein encoded by the femB gene (FemB), and vectors and microorganisms comprising genes encoding the FemB protein.

40. 发明公开

EP0624650A2 FemB gene of Staphylococcus epidermidis, FemB protein, and vectors and microorganisms comprising the FemB gen 失效
标题翻译： FemB-Gen aus葡萄球菌表皮，FemB-Protein，und Vektoren und Mikroorganismen，die das FemB-Gen enthalten。
公开(公告)号：EP0624650A2
公开(公告)日：1994-11-17
申请号：EP94302949.6
申请日：1994-04-25
申请人： ELI LILLY AND COMPANY
发明人： Alborn, Jr., William Ernest , Hoskins, Jo Ann , Skatrud, Paul Luther
IPC分类号： C12N15/31 , C12P21/02 , C07K13/00
CPC分类号： C07K14/31
摘要： The instant invention provides the femB gene of Staphylococcus epidermidis and all degenerate sequences thereof, the protein encoded by the femB gene (FemB), and vectors and microorganisms comprising genes encoding the FemB protein.
摘要翻译：本发明提供了表皮葡萄球菌的femB基因及其所有简并序列，由femB基因（FemB）编码的蛋白质，以及包含编码FemB蛋白的基因的载体和微生物。

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