专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

EP3161150B1 QUANTIFICATION METHODS TO DETERMINE INITIAL TEMPLATE CONCENTRATION BY MEANS OF ASYMMETRIC AMPLIFICATION 失效
标题翻译：用不对称放大法确定初始模板浓度的量化方法
公开(公告)号：EP3161150B1
公开(公告)日：2018-04-18
申请号：EP15733515.9
申请日：2015-06-29
申请人： Epistem Limited
发明人： COBB, Ben
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2531/101 , C12Q2531/107
摘要： Described is a method for quantifying nucleic acid in a nucleic acid amplification reaction, in which an asymmetric nucleic acid amplification reaction is performed on a sample such that double stranded nucleic acid product is generated in an initial stage of the reaction, and single stranded nucleic acid product is generated in a subsequent stage of the reaction once a limiting primer is exhausted. Relative amounts of double stranded nucleic acid product and single stranded nucleic acid product produced are then detected by means of a melt curve analysis. The ratio of double stranded product peak height to single stranded product peak height may then be used to quantify the amount of starting template based on the ratio of double stranded product peak height to single stranded product peak height.

22. 发明公开

EP3194622A1 LINEAR-EXPO-LINEAR PCR (LEL-PCR) 审中-公开
标题翻译： LINEAR-EXPO-LINEAR PCR（LEL-PCR）
公开(公告)号：EP3194622A1
公开(公告)日：2017-07-26
申请号：EP15824507.6
申请日：2015-07-24
申请人： Brandeis University
发明人： WANGH, Lawrence J. , SANCHEZ, Jesus A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12P19/34 , C12Q1/6848 , C12Q2527/101 , C12Q2527/107 , C12Q2527/143 , C12Q2531/101 , C12Q2531/107 , C12Q2523/125
摘要： Disclosed herein is a nucleic acid amplification process referred to as Linear-Expo-Linear Polymerase Chain Reaction (LEL-PCR).
摘要翻译：本文公开了被称为线性 - 博览 - 线性聚合酶链式反应（LEL-PCR）的核酸扩增过程。

23. 发明公开

EP3161150A1 QUANTIFICATION METHODS TO DETERMINE INITIAL TEMPLATE CONCENTRATION BY MEANS OF ASYMMETRIC AMPLIFICATION 失效
标题翻译：定量方法来确定原始模板浓度不对称放大
公开(公告)号：EP3161150A1
公开(公告)日：2017-05-03
申请号：EP15733515.9
申请日：2015-06-29
申请人： Epistem Limited
发明人： COBB, Ben
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2531/101 , C12Q2531/107
摘要： Described is a method for quantifying nucleic acid in a nucleic acid amplification reaction, in which an asymmetric nucleic acid amplification reaction is performed on a sample such that double stranded nucleic acid product is generated in an initial stage of the reaction, and single stranded nucleic acid product is generated in a subsequent stage of the reaction once a limiting primer is exhausted. Relative amounts of double stranded nucleic acid product and single stranded nucleic acid product produced are then detected by means of a melt curve analysis. The ratio of double stranded product peak height to single stranded product peak height may then be used to quantify the amount of starting template based on the ratio of double stranded product peak height to single stranded product peak height.
摘要翻译：描述了一种用于在其中不对称核酸扩增反应的核酸扩增反应定量核酸进行样品上搜索的方法没有双链核酸产物在反应的初始阶段产生的，和单链核酸一旦限制性引物被排出在反应的后级产生的产物。双链核酸产物和产生的单链核酸产物的相对量，然后通过熔融曲线分析来检测。双链产物的峰高到单链产物峰高度的比率然后可被用于量化基于双链产物峰高的单链产物峰高度之比起始模板的量。

24. 发明授权

EP1746172B1 Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids 有权
标题翻译：用PCR后表征用于核酸的标识相关联的非对称PCR
公开(公告)号：EP1746172B1
公开(公告)日：2016-10-05
申请号：EP06013329.5
申请日：2006-06-28
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： Newton, Nicolas , Will, Stephen Gordon
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6851 , C12Q1/6858 , C12Q1/6883 , C12Q1/707 , Y10S435/975 , C12Q2565/101 , C12Q2563/173 , C12Q2527/107 , C12Q2561/113 , C12Q2531/107 , C12Q2561/101 , C12Q2523/319

25. 发明授权

EP2341151B1 Methods for determining sequence variants using ultra-deep sequencing 有权
标题翻译：一种用于确定序列变体的方法使用超深度测序
公开(公告)号：EP2341151B1
公开(公告)日：2015-11-04
申请号：EP10179666.2
申请日：2006-04-12
申请人： 454 Life Sciences Corporation
发明人： Leamon, John, Harris , Lee, William, Lun , Simons, Jan, Fredrick , Desany, Brian , Ronan, Mike, Todd , Drake, James , Lohman, Kenton , Egholm, Michael , Rothberg, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114

26. 发明公开

EP2715347A4 DETECTION OF SEQUENCE VARIANTS IN THE HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) GENE 审中-公开
标题翻译：表皮生长因子受体（EGFR）基因序列变异的证据
公开(公告)号：EP2715347A4
公开(公告)日：2014-12-03
申请号：EP12790203
申请日：2012-05-25
申请人： UNIV BRANDEIS
发明人： WANGH LAWRENCE J , TETRAULT SHANA MARIE , SANCHEZ J AQUILES
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/50
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/686 , C12Q1/6886 , C12Q2600/156 , C12Q2527/107 , C12Q2531/107 , C12Q2565/1015

27. 发明授权

EP2351766B1 Methods for nucleic acid amplification 有权
标题翻译：一种用于核酸扩增法
公开(公告)号：EP2351766B1
公开(公告)日：2014-12-03
申请号：EP10187807.2
申请日：2005-10-17
申请人： BRANDEIS UNIVERSITY
发明人： Wangh, Lawrence J. , Rice, John , Sanchez, J. Aquiles , Pierce, Kenneth , Salk, Jesse , Reis, Arthur , Hartshorn, Cristina
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6851 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107 , C12Q2561/113
摘要： Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labelled primers and probes improves reproducibility and quantification. Low-temperature homogenous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utlilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit "single-tube" processing.

28. 发明授权

EP2406394B1 REAGENTS AND METHODS FOR PCR 有权
标题翻译：试剂和PCR方法
公开(公告)号：EP2406394B1
公开(公告)日：2014-01-08
申请号：EP10708682.9
申请日：2010-03-11
申请人： BRANDEIS UNIVERSITY
发明人： WANGH, Lawrence, J. , RICE, John , RICE, Nicholas , JIA, Yanwei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107
摘要： Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32°C, and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified olgonucleotide being capable of binding to the 5' exonuclease domains of DNA polymerases and, when included in a PCR or other primer-dependent DNA amplification reaction at a concentration, generally not more than 2000 nM, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3' terminal mismatches, increasing polymerase selectivity against AT-rich 3' ends, reducing scatter among replicates, suppressing polymerase 5' exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides.

29. 发明公开

EP2543737A4 METHOD FOR DETECTING NUCLEIC ACIDS BY PROMOTING BRANCHED DNA COMPLEX FORMATION 有权
标题翻译：通过促进分支DNA复合物形成来检测核酸的方法
公开(公告)号：EP2543737A4
公开(公告)日：2013-10-23
申请号：EP11750911
申请日：2011-03-03
申请人： K MAC
发明人： CHAE CHI-BOM , KIM KYUNG-TAE , KANG JONG HUN
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/686 , C12Q2525/313 , C12Q2523/305 , C12Q2527/107 , C12Q2531/107 , C12Q2547/101

30. 发明公开

EP2495334A4 METHOD FOR DETECTION OF TARGET NUCLEIC ACID 有权
标题翻译：检测方法的靶核酸的
公开(公告)号：EP2495334A4
公开(公告)日：2013-08-21
申请号：EP10826718
申请日：2010-10-26
申请人： NGK INSULATORS LTD
发明人： NIWA KOUSUKE , HIROTA TOSHIKAZU , KAWASE MITSUO
IPC分类号： C12Q1/68 , C12M1/00 , C12N15/09 , G01N33/53 , G01N33/543 , G01N37/00
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/158 , C12Q2531/107 , C12Q2537/143 , C12Q2563/107 , C12Q2565/514
摘要： An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer 30 comprising an identification sequence 32 complementary to a target sequence 12 in a target nucleic acid and a tag addition sequence 36, and a second primer 40 having a label 42 are prepared. The first primer 30 and the second primer 40 are used for the target nucleic acid 10 in a sample to amplify a chimeric DNA 60 having a tag sequence 66 and the label 42. The chimeric DNA 60 is hybridized with a detection probe 104 on a solid phase 100 to obtain signal intensity information based on the label 42, and the target nucleic acid 10 is detected based on the signal intensity information.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式