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21. 发明公开

EP2966435A1 RESIN PARTICLES FOR FLUORESCENT LABELS 审中-公开
标题翻译：树脂颗粒用于荧光标签
公开(公告)号：EP2966435A1
公开(公告)日：2016-01-13
申请号：EP14761005.9
申请日：2014-03-04
申请人： Konica Minolta, Inc.
发明人： TAKAHASHI, Masaru , TAKANASHI, Kensaku
IPC分类号： G01N21/64 , C08J3/14 , C08J3/20 , C08L101/00 , C09B67/02
CPC分类号： G01N33/533 , C08L101/00 , C09B67/001 , C09B67/0017 , G01N1/30 , G01N21/6428 , G01N33/52 , G01N2001/302 , G01N2021/6441 , G01N2458/00 , G01N2800/7028
摘要： An object of the present invention is to provide a fluorophore having excellent initial emission intensity and light resistance, which is not easily discolored and does not affect the evaluation of the number of bright spots even when it is slightly discolored. The resin particle for fluorescence labeling is characterized by comprising a fluorescent dye immobilized in a resin particle, the fluorescent dye satisfying both of the following conditions (1) and (2): (1) the fluorescent dye alone in an aqueous solution has a concentration-dependent peak emission intensity in a range of 30 to 80 µM; and (2) the emission intensity at a concentration of 100 µM is not less than 80% of the peak emission intensity. It is preferred that the Stoke's shift of the fluorescent dye in an aqueous solution is not less than 25 nm. The fluorescent dye may be subjected to a solubilization treatment (such as an acid treatment). It is preferred that the fluorescent dye be encapsulated in a resin particle comprising a thermosetting resin.
摘要翻译：本发明的目的在于提供初始发光强度和耐光性优异，即使稍微变色也不容易变色，并且不影响亮点数量的评价的荧光体。荧光标记用树脂粒子的特征在于，包含固定在树脂粒子中的荧光染料，该荧光染料满足以下两个条件（1）和（2）：（1）仅在水溶液中的荧光染料的浓度依赖的峰值发射强度范围为30至80μM; 和（2）浓度为100μM时的发射强度不小于峰值发射强度的80％。荧光染料在水溶液中的斯托克位移优选不小于25nm。荧光染料可以进行增溶处理（例如酸处理）。荧光染料优选被包封在包含热固性树脂的树脂颗粒中。

22. 发明公开

EP2755019A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 有权转让
标题翻译： VERFAHREN ZUM NACHWEIS生物学家STOFFE
公开(公告)号：EP2755019A1
公开(公告)日：2014-07-16
申请号：EP12829813.0
申请日：2012-09-04
申请人： Konica Minolta, Inc. , Tohoku University
发明人： TAKANASHI, Kensaku , OKADA, Hisatake , NAKANO, Yasushi , GONDA, Kohsuke , OHUCHI, Noriaki , WATANABE, Mika
IPC分类号： G01N21/64
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/56966 , G01N33/57492 , G01N2021/6439 , G01N2333/70596
摘要： The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.
摘要翻译：本发明提供了一种用于特异性检测来自病理标本的生物物质的生物物质检测方法，通过该方法，当使用荧光标记物进行免疫染色并同时进行用于形态学观察的染色剂的形态观察染色时，荧光检测结果即使通过用激发光照射使荧光标记和/或染色剂劣化，也可以适当地评价观察和免疫染色。根据本发明的生物物质检测方法的特征在于，当荧光标记物的形态学观察时，与染色的部分的亮度保持率相比，免疫染色部分的亮度保持率在80％至120％的范围内用于免疫染色观察。

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