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    • 12. 发明公开
    • APPARATUS FOR THE SUSPENSION OF CULTURED CELLS FROM TISSUES AND MICRO-ORGANISMS
    • 设计祖国悬崖薇薇薇
    • EP1120460A1
    • 2001-08-01
    • EP98963676.6
    • 1998-09-22
    • Zakrytoe Aktsionernoe Obschestvo "SAYANY"
    • KISLYKH, Vasily, IvanovichRAMAZANOV, Jury, AkhmetovichREPKOV, Andrei, Petrovich
    • C12M1/04C12M3/00
    • C12M23/56C12M27/00C12M29/00Y10S435/818
    • The present invention relates to biotechnology, and more particularly to an apparatus for cultivating tissue cells or microorganisms in suspension. The apparatus provides cultivating tissue cells or microorganisms having different oxygen requirements. The apparatus comprises a cylindrical reservoir (1) with a cover (2) and branch pipes (3) and (4), respectively, for supplying an aerating gas and removing gaseous medium, and a device for aerating and stirring the medium, comprising a horizontal blade wheel (5) secured on a vertical hollow shaft (6) and arranged in the upper part of the reservoir (1) directly under the cover (2), an annular partition (7) installed in the cylindrical reservoir (1) coaxially with the blade wheel (5) with a clearance between the cylindrical wall of the reservoir (1) and the annular partition (7), and a mechanism for stabilizing the position of the annular partition with respect to the surface of the liquid phase, said mechanism comprising guide elements (11) and floats (10). The guide elements (11) of the mechanism for stabilizing the position of the annular partition (7) with respect to the surface of the liquid are made as detachable blades (12 and 13) with a flat upper surface (14) and a convex lower surface (15), said blades (12 and 13) being oriented radially with respect to the annular partition (7) and the surfaces (14) and (15) of said blades (12 and 13) defining an aerodynamic profile thereof of the "forward-sweep wing" type; the blades (12) are attached with the help of struts (16) to the upper surface (9) of the annular partition (7) or the blades (12) are attached to the upper surface (9) of the partition (7) and the blades (13) are attached to the lower surface (8) of the partition (7) with the help of struts (17), the blades (12) and (13) being provided with units for varying the angle of attack with respect to the incoming flow of cas or liquid and for securing the blades (12) and (13), respectively, to the struts (16) and (17) on the annular partition (7) and under it.
    • 本发明涉及生物技术,更具体地涉及用于培养悬浮液中的组织细胞或微生物的装置。 该装置提供具有不同氧需求的培养组织细胞或微生物。 该装置包括分别具有盖(2)和分支管(3)和(4)的圆柱形容器(1),用于供应充气气体和除去气体介质,以及用于对介质进行充气和搅拌的装置,包括 水平叶轮(5)固定在垂直空心轴(6)上,并且直接位于盖(2)的下面的储存器(1)的上部,同轴地安装在圆柱形储存器(1)中的环形隔板 其中所述叶轮(5)在所述储存器(1)的圆柱形壁与所述环形隔板(7)之间具有间隙,以及用于相对于所述液相的表面稳定所述环形隔板的位置的机构,所述 机构包括引导元件(11)和浮子(10)。 用于使环形隔板(7)相对于液体表面的位置稳定的机构的引导元件(11)被制成具有平坦的上表面(14)和凸起的下部的可拆卸的叶片(12和13) 表面(15),所述叶片(12和13)相对于环形分隔件(7)径向定向,并且所述叶片(12和13)的表面(14)和(15)限定其“ 前掠翼“型; 叶片(12)借助于支柱(16)附接到环形隔板(7)的上表面(9),或者叶片(12)附接到隔板(7)的上表面(9) 并且叶片(13)借助于支柱(17)附接到隔板(7)的下表面(8),叶片(12)和(13)设置有用于改变迎角的单元 相对于壳体或液体的进入流动并且用于将叶片(12)和(13)分别固定到环形分隔件(7)上及其下方的支柱(16)和(17)上。