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    • 16. 发明授权
    • A family of pesticidal proteins and methods for their use
    • Pestizidproteinfamilie und Verwendungsverfahrendafür
    • EP2455394B1
    • 2017-04-12
    • EP11173106.3
    • 2007-06-15
    • Athenix Corporation
    • Koziel, Michael, GDesai, NaliniDeter, RebekahTomso, Daniel, JohnHargiss, TracyDuck, Nicholas, B.Carozzi, NadineGuo, Rong
    • C07K14/195C07K14/32C12N15/82
    • C12N15/8286A01N37/44C07K14/325C12P21/02Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 13, 2, 4, 6, 7, 9, 11, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 12, 1, 3, 5, 8, 10, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包含对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所示的核苷酸序列,SEQ ID NO:12,3,3,5,8,10,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。
    • 17. 发明公开
    • A FAMILY OF PESTICIDAL PROTEINS AND METHODS FOR THEIR USE
    • VERFAHREN ZU DEREN VERWENDUNG的SCHÄDLINGSBEKÄMPFENDEPROTEINFAMILIE
    • EP3150625A2
    • 2017-04-05
    • EP16194435.0
    • 2007-06-15
    • Athenix Corporation
    • KOZIEL, Michael, G.DESAI, NaliniDETER, RebekahTOMSO, Daniel, JohnHARGISS, TracyDUCK, Nicholas, B.CAROZZI, NadineGUO, Rong
    • C07K14/195C07K14/32C12N15/82
    • C12N15/8286A01N37/44C07K14/325C12P21/02Y02A40/162
    • Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.
    • 提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包含对应于多核苷酸的氨基酸序列。 具体地说,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所示的核苷酸序列,如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-50047,B-30959,B-30960,B-30943,B-50048或B-50048,以及其变体和片段。