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    • 92. 发明公开
    • Liquid chromatography system and method for thermally stabilizing several columns of a liquid chromatography system
    • 液相色谱系统和方法,用于液相色谱的若干列的热稳定
    • EP0716302A2
    • 1996-06-12
    • EP95116934.1
    • 1995-10-27
    • Hewlett-Packard GmbH
    • Anderer, HerbertDehmer, BernhardDinter, RaoulGlatz, BerndKoch, FriedhelmKrämer, Karl-Heinz
    • G01N30/30
    • G01N30/30G01N2030/027G01N2030/3046G01N2030/3007G01N2030/085
    • A method for thermally stabilizing two columns for liquid chromatography comprises the following steps: operating the first column at a first temperature which is different from the operating temperature of the second column, and transferring the sample contained in the first column to the second column by operation of switching means arranged between the two columns. In an embodiment of the invention, the first column is a pre-column (102) and the pre-column is operated, during an enrichment phase, at a first temperature lower than an operating temperature of the separation column, in order to maintain a sample at a first temperature, the sample contained in the pre-column is heated, after completion of the enrichment phase, to a second temperature higher than the operating temperature of the separation column (104), then the sample contained in the pre-column (102) is transferred to the separation column and then the sample is cooled to the operating temperature of the separation column.
    • 一种用于热稳定两列用于液相色谱方法包括以下步骤:在第一温度下的所有这是从第二塔的工作温度不同操作所述第一列中,并且传递环包含在第一列到通过外科手术第二列中的样品 切换两列之间设置的装置。 在本发明的一个实施例中,第一列是预柱(102)和预柱被操作,在此期间在浓缩阶段,在第一温度下比在分离柱的操作温度低,为了维持一个 在第一温度下的样品中,包含在预柱的样品加热,富集阶段完成后,到第二温度比所述分离塔(104)的工作温度越高,则包含在所述预柱的样品 (102)被转移到分离柱,然后将样品冷却至分离塔的操作温度。