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91. 发明公开

EP3617916A1 INTEGRATION SYSTEM AND INTEGRATION METHOD 审中-实审
公开(公告)号：EP3617916A1
公开(公告)日：2020-03-04
申请号：EP18790917.1
申请日：2018-04-27
申请人： Japan Agency for Marine-Earth Science and Technology , Technosuruga Laboratory Co.,Ltd.
发明人： KITAHASHI, Tomo , NISHIJIMA, Miyuki , SHIMOTASHIRO, Masafumi
IPC分类号： G06F19/28 , C12Q1/6869 , G06F17/30 , G06Q50/10
摘要： An integration system comprising a biological image acquiring device which acquires, from a sample including biological particles which are a detection target, a biological image which is an image of the biological particles; a nucleotide sequence information acquiring device which acquires nucleotide sequence information of the biological particles; and an integration device which associates and registers the biological image and the nucleotide sequence information acquired from the same type of biological particles in an integrated database.

92. 发明公开

EP3378948A1 METHOD FOR QUANTIFYING TARGET NUCLEIC ACID AND KIT THEREFOR 有权
公开(公告)号：EP3378948A1
公开(公告)日：2018-09-26
申请号：EP16866395.3
申请日：2016-11-17
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： HOSHINO, Tatsuhiko , INAGAKI, Fumio
IPC分类号： C12Q1/68 , C12N15/09
摘要： The purpose of the present invention is to provide a method for allowing both sequencing and quantification of a target nucleic acid without performing a ligation reaction and while solving a problem which arises due to PCR amplification efficiency. The method comprises (1) performing a single primer extension reaction using a primer having a sequence specific to the sequence of a target nucleic acid, and a random sequence and a known sequence which are introduced upstream of that specific sequence, thereby obtaining a double-stranded nucleic acid having the random sequence and the sequence of the target nucleic acid, (2) performing PCR using the obtained double-stranded nucleic acid as a template, and a primer specific to a known sequence and a primer having a portion of the sequence of the target nucleic acid, thereby obtaining an amplified product of the double-stranded nucleic acid having the random sequence and the sequence of the target nucleic acid, and (3) performing sequencing using the obtained amplified product of the double-stranded nucleic acid, thereby decoding the sequence of the random sequence as well as the target nucleic acid.

93. 发明公开

EP3153982A1 PARTICLE SIMULATION DEVICE, PARTICLE SIMULATION METHOD, AND PARTICLE SIMULATION PROGRAM 审中-实审
标题翻译：差异分析法，分类法分析法
公开(公告)号：EP3153982A1
公开(公告)日：2017-04-12
申请号：EP15803426.4
申请日：2015-05-29
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： YAMAMOTO, Miki , NISHIURA, Daisuke , SAKAGUCHI, Hide
IPC分类号： G06F19/00
CPC分类号： G06G7/485 , G01N15/10 , G01N2015/0003 , G01T5/002 , G06F17/10 , G06F17/5009 , G06F17/5018 , G06F19/00 , G06F19/12 , G06F19/701 , Y02A90/16
摘要： The calculation efficiency is improved in a particle simulation. A particle simulation device 10, which calculates the position and velocity of particles on the basis of the interaction force between particles in a work space, comprises: a position information acquisition unit 12 that acquires position information for the particles; a particle number setting unit 13 that sets particle numbers for the particles; a pair setting unit 14 that selects pairs of particles and sets a pair number, on the basis of the position information; a reference information generation unit 15 that generates a matrix from the pair numbers and the particle numbers, and generates reference information for referencing the pair numbers from the particle numbers on the basis of a matrix in which the order of the rows of the matrix are sorted on the basis of the particle numbers; a contact force calculation unit 16 that calculates the interaction force of the particles in the pairs; a sum calculation unit 17 that calculates a sum of interaction forces for each particle on the basis of the reference information; and a particle information calculation unit 18 that calculates the position and velocity of the particles on the basis of the sum of interaction forces.
摘要翻译：在粒子模拟中计算效率得到提高。颗粒模拟装置10，其基于工作空间中的颗粒之间的相互作用力计算颗粒的位置和速度，包括：位置信息获取单元12，其获取颗粒的位置信息; 设置粒子的粒子数的粒子数设定部13; 一对设定单元14，根据位置信息选择一对粒子并设定一对; 参考信息生成单元15，其从对号和粒子数生成矩阵，并且基于矩阵的排列顺序从矩阵生成参照来自粒子数的对数的参考信息，基于粒子数; 接触力计算单元16，其计算成对中的颗粒的相互作用力; 和计算单元17，其基于参考信息计算每个粒子的相互作用力的总和; 和粒子信息计算单元18，其基于相互作用力的和来计算粒子的位置和速度。

94. 发明授权

EP2617631B1 CRAWLER DEVICE AND CRAWLER ROBOT 有权
标题翻译：跟踪设备和跟踪机器人
公开(公告)号：EP2617631B1
公开(公告)日：2017-04-05
申请号：EP11825243.6
申请日：2011-09-15
申请人： Independent Administrative Institution Japan Agency for Marine-Earth Science and Technology , Topy Kogyo Kabushiki Kaisha
发明人： INOUE, Tomoya , TSUKUI, Shingo , FUJIMOTO, Katsuki
IPC分类号： B62D55/08 , B62D55/088 , B62D55/12 , B62D55/24
CPC分类号： B62D55/24 , B60C7/24 , B60F3/0015 , B62D55/00 , B62D55/04 , B62D55/084 , B62D55/088 , B62D55/12 , B62D55/14 , B62D55/205 , Y10S901/01

95. 发明公开

EP3106523A1 METHOD FOR PRODUCING PHENYL PROPANE-BASED COMPOUND USING MICRO-ORGANISMS 有权
标题翻译：用微生物制备苯基丙烷基化合物的方法
公开(公告)号：EP3106523A1
公开(公告)日：2016-12-21
申请号：EP15746820.8
申请日：2015-02-09
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： OHTA, Yukari , HATADA, Yuji , HASEGAWA, Ryoichi
IPC分类号： C12P7/22 , C12N1/20 , C12N15/09 , C12R1/01
CPC分类号： C12P7/22 , C12N1/20 , C12P7/24 , C12R1/01
摘要： ABSTRACT
The object of the invention is to provide a method which specifically and efficiently produces a compound having a phenol propane structure from natural biomass containing lignins by causing microorganisms to act on biomass. The object is achieved by a method for producing a phenyl propane-based compound comprising a step of producing a phenyl propane-based compound by causing microorganisms of the genus Novosphingobium to act on biomass containing lignins and/or lignin-related substances.
摘要翻译：摘要本发明的目的是提供一种方法，其通过使微生物作用于生物质而从含有木质素的天然生物质中特异性且高效地生产具有苯酚丙烷结构的化合物。该目的通过用于制备苯基丙烷类化合物的方法来实现，该方法包括通过使Novosphingobium属微生物作用于含有木质素和/或木质素相关物质的生物质来制备苯基丙烷类化合物的步骤。

96. 发明公开

EP2674517A1 METHOD FOR PRODUCING CALCITE SINGLE CRYSTAL 审中-公开
标题翻译： VERFAHREN ZUR HERSTELLUNG EINES CALCIT-EINKRISTALLS
公开(公告)号：EP2674517A1
公开(公告)日：2013-12-18
申请号：EP12744739.9
申请日：2012-02-08
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： YANAGISAWA, Kazumichi , SAKAGUCHI, Arito , SAKAGUCHI, Hide
IPC分类号： C30B29/10 , C01F11/18 , C30B7/08
CPC分类号： C30B7/08 , C30B29/10
摘要： The objective of the present invention is to provide a method for producing a calcite single crystal which is appropriately fine in a large scale with no difficulty. The method for producing a calcite single crystal according to the present invention is characterized in comprising the steps of mixing raw material calcium carbonate with an ammonium nitrate aqueous solution to obtain a raw material mixed liquid, wherein pH of the ammonium nitrate aqueous solution is not less than 7.0 and not more than 8.0, a concentration of the ammonium nitrate aqueous solution is not less than 2 M, and a concentration of calcium carbonate in the raw material mixed liquid is not less than 4 g/L and not more than 16 g/L; heating the raw material mixed liquid at not less than 125°C; and then gradually cooling the raw material mixed liquid.
摘要翻译：本发明的目的是提供一种大规模地适当地精细地生产方解石单晶的方法。本发明的方解石单晶的制造方法的特征在于，将原料碳酸钙与硝酸铵水溶液混合，得到原料混合液，硝酸铵水溶液的pH值不小于 7.0以上8.0以下，硝酸铵水溶液的浓度为2m以上，原料混合液中的碳酸钙浓度为4g / L以上16g / L以下，升; 在不低于125℃下加热原料混合液; 然后逐渐冷却原料混合液。

97. 发明公开

EP2554670A1 PLASMID VECTOR 审中-公开
标题翻译：等离子矢量
公开(公告)号：EP2554670A1
公开(公告)日：2013-02-06
申请号：EP11765369.1
申请日：2011-03-18
申请人： Independent Administrative Institution Japan Agency for Marine-Earth Science and Technology
发明人： OHTA Yukari , HATADA Yuji , MORI Kozue , NAKAMURA Nobuyuki
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10
CPC分类号： C12N15/74
摘要： The object is to provide a technique for modifying a bacterium belonging to the genus Kocuria through genetic engineering for industrially effectively utilizing the bacterium belonging to the genus Kocuria. The above object can be achieved by providing a cyclic plasmid, which has a replication region comprising the base sequence of a DNA-binding protein-like protein gene, the base sequence of a replicase-like protein gene, and the base sequence represented by SEQ ID NO:45, and is autonomously replicable in bacteria. As an example of the aforesaid plasmid, a plasmid containing a base sequence represented by SEQ ID NO:3 or 4 which originates in Kocuria sp. MBE131 strain (FERM P-21885) can be cited.
摘要翻译：本发明的目的是提供一种通过基因工程修饰属于Kocuria属的细菌以工业上有效地利用属于Kocuria属的细菌的技术。上述目的可以通过提供一种环状质粒来实现，该环状质粒具有包含DNA结合蛋白质样蛋白质基因的碱基序列，复制酶样蛋白质基因的碱基序列和由SEQ ID表示的碱基序列的复制区域 ID号：45，可以在细菌中自主复制。作为上述质粒的例子，可以使用含有来源于Kocuria sp。的SEQ ID NO：3或4所示的碱基序列的质粒。可以引用MBE131菌株（FERM P-21885）。

98. 发明公开

EP2495309A1 METHOD FOR PREPARATION OF PROLIFERATABLE ANIMAL CELLS 审中-公开
标题翻译： VERFAHREN ZUR HERSTELLUNG PROLIFERIERBARER TIERZELLEN
公开(公告)号：EP2495309A1
公开(公告)日：2012-09-05
申请号：EP10826847.5
申请日：2010-10-29
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： KOYAMA, Sumihiro
IPC分类号： C12N5/071 , A61L27/00 , C12M3/00 , C12N5/07
CPC分类号： C12N5/00 , C12M25/08 , C12M33/00 , C12M35/02 , C12N2529/00 , C12N2539/10
摘要： A method for preparing animal cells with good adhesion and proliferation properties which are capable of proliferation is provided. the method permits the detachment of cultured cells without damaging the cells. A method for preparing a sheet of animal cells such as skin cells which are capable of proliferation is also provided. A method for preparing animal cells capable of proliferation, comprising the steps of (1) culturing animal cells on a substrate at least one portion of which is an electrode, and (2) applying a high-frequency wave potential to the electrode to detach the cells that have adhered to the substrate surface through culturing. The high-frequency wave potential is of a frequency falling within a range of 1 KHz to 10 MHz with a potential falling within a range of ± 1.0 V (vs. Ag/AgCl) or less. The culture medium during separation does not contain calcium or magnesium.
摘要翻译：提供了一种制备具有良好粘附性和增殖性能的能够增殖的动物细胞的方法。该方法允许培养的细胞脱离而不破坏细胞。还提供了一种制备能够增殖的皮肤细胞的动物细胞片的方法。一种制备能够增殖的动物细胞的方法，包括以下步骤：（1）在基材上培养动物细胞，其中至少一部分是电极，和（2）向所述电极施加高频波电位以分离通过培养粘附于基材表面的细胞。高频波电位为1KHz〜10MHz的范围，电位落在±1.0V（相对于Ag / AgCl）以下的范围内。分离过程中的培养基不含钙或镁。

99. 发明公开

EP2481666A1 FLOAT DEVICE 有权
标题翻译： SCHWIMMVORRICHTUNG
公开(公告)号：EP2481666A1
公开(公告)日：2012-08-01
申请号：EP10826779.0
申请日：2010-10-27
申请人： The Tsurumi Seiki Co., Ltd. , Japan Agency for Marine-Earth Science and Technology
发明人： WATANABE, Kazuhiro , SHIKAMA, Nobuyuki , KEISUKE,Mizuno
IPC分类号： B63B22/08
CPC分类号： B63G8/22 , B63B22/20 , B63B2211/02 , B63G8/24
摘要： There is provided a float device capable of reducing the number of parts and controlling a buoyant force during both ascent and descent with high accuracy, the float device includes a float hull 11 having a certain buoyancy, a drive motor 44 provided inside the float hull 11, a plunger 44 reciprocating along with rotation of the drive motor 41, an internal oil reservoir 50 for storing an hydraulic oil therein, a cylinder 71 attached to the float hull 11 and opened to the outside, a buoyant force adjustment piston 72 reciprocating in the cylinder 71 along with exit/entry of the hydraulic oil, and a three-way valve 61 having a first connection port 61a connected to the plunger 44, a second connection port 61b connected to the internal oil reservoir 50, and a third connection port 61c connected to the cylinder 71, for switching the flows of the first connection port 61a and the second connection ports 61b and the flows of the first connection port 61a and the third connection port 61c.
摘要翻译：提供了一种能够以高精度减少上升和下降的部件数量并控制浮力的浮子装置，浮子装置包括具有一定浮力的浮子船体11，设置在浮动船体11内的驱动马达44 与驱动马达41的旋转一起往复运动的柱塞44，用于在其中储存液压油的内部储油器50，安装在浮体壳体11上并向外部开口的气缸71，在其中往复运动的浮力调节活塞72 气缸71以及液压油的出入口和具有连接到柱塞44的第一连接口61a的三通阀61，连接到内部储油器50的第二连接口61b和第三连接口61c 连接到气缸71，用于切换第一连接口61a和第二连接口61b的流动以及第一连接口61a和第三连接口61c的流动。

100. 发明公开

EP2374880A1 METHOD FOR INHIBITING PROLIFERATION OF CELLS 有权
标题翻译：方法抑制细胞增殖
公开(公告)号：EP2374880A1
公开(公告)日：2011-10-12
申请号：EP09830195.5
申请日：2009-12-02
申请人： Japan Agency for Marine-Earth Science and Technology
发明人： KOBAYASHI, Hideki
IPC分类号： C12N15/09 , A61K48/00 , A61P31/12 , A61P35/00
CPC分类号： C12N15/67 , A61K48/005 , C12N15/70 , C12N2830/002
摘要： Disclosed is a method for suppressing the growth of a target cell, which is not limited in the type of a target cell and the type of a protein to be expressed in the target cell and needs not any preparatory experiment for determining a codon to be contained in a protein to be expressed in a target cell. Specifically disclosed is a method for suppressing the growth of a target cell, which comprises the steps of: incorporating DNA containing a region encoding a protein into the target cell, and allowing a protein encoded by the DNA to be expressed in the target cell into which the DNA has been incorporated. The region contained in the DNA comprises a tri-nucleotide sequence. The tri-nucleotide sequence is selected from codons that define at least some amino acid species constituting the protein, and is complementary to at least some codons that are used in the target cell at a frequency of 0.2 or less.

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