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1. 发明授权

US06653094B2 Indicators of altered mitochondrial function in predictive methods for determining risk of type 2 diabetes mellitus 失效
公开(公告)号：US06653094B2
公开(公告)日：2003-11-25
申请号：US09924313
申请日：2001-08-07
申请人： Christen M. Anderson , Robert E. Davis
发明人： Christen M. Anderson , Robert E. Davis
IPC分类号： C12Q132
CPC分类号： C12Q1/6883 , G01N33/6893 , G01N2800/042
摘要： The present invention relates to improved diagnostic methods for early detection of a risk for developing type 2 diabetes mellitus in humans, and screening assays for therapeutic agents useful in the treatment of type 2 diabetes mellitus, by comparing the levels of one or more indicators of altered mitochondrial function. Indicators of altered mitochondrial function include enzymes such as mitochondrial enzymes and ATP biosynthesis factors. Other indicators of altered mitochondrial function include mitochondrial mass, mitochondrial number and mitochondrial DNA content, cellular responses to elevated intracellular calcium and to apoptogens, and free radical production. Methods of treating, and of stratifying, human patients as such methods relate to disclosed indicators of altered mitchondrial function are also provided.

2. 发明授权

US06544756B1 Sorbitol dehydrogenase, microorganism for producing same, process for the production thereof, method for the measurement of sorbitol and reagent for the quantitative determination therefor 失效
标题翻译：山梨糖醇脱氢酶，其制造用微生物，其制造方法，山梨糖醇的测定方法和定量测定用试剂
公开(公告)号：US06544756B1
公开(公告)日：2003-04-08
申请号：US09645356
申请日：2000-08-25
申请人： Michie Uchida , Ryoko Nakatani , Keisuke Kurosaka , Shido Kawase , Miwa Watanabe
发明人： Michie Uchida , Ryoko Nakatani , Keisuke Kurosaka , Shido Kawase , Miwa Watanabe
IPC分类号： C12Q132
CPC分类号： C12N9/0006 , C12Q1/32 , C12R1/39 , C12R1/40 , Y10S435/85
摘要： A novel sorbitol dehydrogenase having excellent substrate affinity and substrate specificity which can be used to measure D-sorbitol which occurs in human erythrocytes and serum in a slight amount or D-sorbitol or D-fructose contained in foods, a microorganism for producing such an enzyme, and a process for the production of the enzyme using such a microorganism. Also disclosed is provides a method for the measurement of sorbitol using the foregoing sorbitol dehydrogenase and a reagent for the quantitative determination therefor.
摘要翻译：具有优异的底物亲和性和底物特异性的新型山梨糖醇脱氢酶，其可用于测量食品中含有的微量的人红细胞和血清中的D-山梨糖醇，D-山梨糖醇或D-果糖，用于生产该酶的微生物，以及使用这种微生物生产酶的方法。还公开了使用上述山梨糖醇脱氢酶和用于定量测定的试剂测量山梨糖醇的方法。

3. 发明授权

US06280966B1 Indicators of altered mitochondrial function in predictive methods for determining risk of type 2 diabetes mellitus 失效
标题翻译：线粒体功能改变的指标，用于确定2型糖尿病风险的预测方法
公开(公告)号：US06280966B1
公开(公告)日：2001-08-28
申请号：US09521407
申请日：2000-03-08
申请人： Christen M. Anderson , Robert E. Davis
发明人： Christen M. Anderson , Robert E. Davis
IPC分类号： C12Q132
CPC分类号： C12Q1/6883 , G01N33/6893 , G01N2800/042
摘要： The present invention relates to improved diagnostic methods for early detection of a risk for developing type 2 diabetes mellitus in humans, and screening assays for therapeutic agents useful in the treatment of type 2 diabetes mellitus, by comparing the levels of one or more indicators of altered mitochondrial function. Indicators of altered mitochondrial function include enzymes such as mitochondrial enzymes and ATP biosynthesis factors. Other indicators of altered mitochondrial function include mitochondrial mass, mitochondrial number and mitochondrial DNA content, celullar responses to elevated intracellular calcium and to apoptogens, and free radical production. Methods of treating, and of stratifying, human patients as such methods relate to disclosed indicators of altered mitchondrial function are also provided.
摘要翻译：本发明涉及用于早期检测人类发展中的2型糖尿病的风险的改进的诊断方法，以及用于治疗2型糖尿病的治疗药物的筛选测定法，通过比较一种或多种改变的线粒体功能。改变线粒体功能的指标包括线粒体酶和ATP生物合成因子等酶。线粒体功能改变的其他指标包括线粒体质量，线粒体数量和线粒体DNA含量，细胞内钙升高和凋亡细胞的纤溶酶反应以及自由基生成。还提供了用于治疗和分层人类患者的方法，这些方法涉及公开的改变的脑膜功能指标。

4. 发明授权

US06541216B1 Amperometric biosensor test strip 有权
标题翻译：电流式生物传感器试片
公开(公告)号：US06541216B1
公开(公告)日：2003-04-01
申请号：US09469899
申请日：1999-12-22
申请人： Christopher Douglas Wilsey , David W. Burke
发明人： Christopher Douglas Wilsey , David W. Burke
IPC分类号： C12Q132
CPC分类号： C12Q1/005 , Y10S435/817
摘要： A reagent and method for determining the levels of 3-hydroxybutyric acid in a sample are provided. The reagent comprises a ferricyanide salt, a catalytic amount of a first enzyme operative to catalyze the oxidation of 3-hydroxybutyric acid in the sample, a cofactor corresponding to said first enzyme, and a catalytic amount of a second enzyme operative to catalyze the oxidization of the cofactor and the reduction of the ferricyanide. The reagent is incorporated into a test strip that generates an electrical output signal indicative of the level of 3-hydroxybutyric acid when the reagent is contacted with a sample.
摘要翻译：提供了用于测定样品中3-羟基丁酸水平的试剂和方法。该试剂包括铁氰化物盐，催化量的第一酶，其可操作以催化样品中3-羟基丁酸的氧化，对应于所述第一种酶的辅因子，和催化量的第二种酶，其可催化氧化辅助因子和铁氰化物的还原。当试剂与样品接触时，将试剂并入测试条中，其产生指示3-羟基丁酸水平的电输出信号。

5. 发明授权

US06524808B1 Enzyme inhibition immunoassay 有权
公开(公告)号：US06524808B1
公开(公告)日：2003-02-25
申请号：US09603646
申请日：2000-06-26
申请人： Allan R. Dorn , Salvatore J. Salamone , Mitali Ghoshal , Eva Hoess , Erasmus Huber , Ronald C. Hawley , John W. Patterson
发明人： Allan R. Dorn , Salvatore J. Salamone , Mitali Ghoshal , Eva Hoess , Erasmus Huber , Ronald C. Hawley , John W. Patterson
IPC分类号： C12Q132
CPC分类号： G01N33/5735 , G01N33/78 , G01N2333/904 , Y10S435/975
摘要： The present invention provides a method for the measurement of an analyte in biological samples whereby an uncompetitive inhibitor is coupled to a ligand and utilized in a homogeneous assay. The analyte can be a drug or drug derivative, hormone, polypeptide, or oligonucleotide. The present invention also provides novel compounds, assay reagents and packaged kits useful for performing such measurements.

6. 发明授权

US06200773B1 Diagnostics based on tetrazolium compounds 有权
标题翻译：基于四唑化合物的诊断
公开(公告)号：US06200773B1
公开(公告)日：2001-03-13
申请号：US09282083
申请日：1999-03-30
申请人： Tianmei Ouyang , Yeung Siu Yu
发明人： Tianmei Ouyang , Yeung Siu Yu
IPC分类号： C12Q132
CPC分类号： G01N33/525 , C12Q1/32 , G01N33/64 , Y10S435/962 , Y10S435/97 , Y10T436/173076
摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.
摘要翻译：试剂适合于测量含血红蛋白的生物液体如全血中的分析物的浓度。该试剂包含对分析物，NAD，NAD衍生物，吡咯并喹啉醌（PQQ）或PQQ衍生物，四唑染料前体，心肌黄酶或其类似物和亚硝酸盐具有特异性的脱氢酶。试剂导致染料形成，这是测量分析物浓度。亚硝酸盐抑制由血红蛋白非酶促引起的干扰性染料形成。优选地，试剂用于测量酮体例如β-羟基丁酸酯的干燥条。

7. 发明授权

US06762035B1 Method and test strips for the measurement of fat loss during weight loss programs 失效
标题翻译：减肥计划中测量脂肪损失的方法和测试条
公开(公告)号：US06762035B1
公开(公告)日：2004-07-13
申请号：US10067660
申请日：2002-02-04
申请人： Surendra K. Gupta
发明人： Surendra K. Gupta
IPC分类号： C12Q132
CPC分类号： G01N33/523 , C12Q1/32 , G01N2800/044 , Y10S435/829 , Y10S435/968 , Y10S435/97
摘要： A method for monitoring the progress of fat loss in a patient during a weight loss program which comprises, contacting a body fluid sample from said patient with a solid test strip to provide a color indication of the presence in said body fluid of &bgr;-hydroxybutyrate, optionally together with acetoacetate and/or acetone.
摘要翻译：一种用于在减肥计划期间监测患者的脂肪减少进展的方法，其包括：使来自所述患者的体液样品与固体测试条接触，以提供在β-羟基丁酸酯的所述体液中存在的颜色指示，任选地与乙酰乙酸酯和/或丙酮一起。

8. 发明授权

US06720164B1 Method of determining substrate, and biosensor 失效
标题翻译：确定底物和生物传感器的方法
公开(公告)号：US06720164B1
公开(公告)日：2004-04-13
申请号：US09914292
申请日：2001-08-27
申请人： Naoki Shinozuka , Toru Yokoyama , Kenji Nakamura
发明人： Naoki Shinozuka , Toru Yokoyama , Kenji Nakamura
IPC分类号： C12Q132
CPC分类号： C12Q1/005
摘要： A method of quantifying a substrate, by which the substrate contained in various samples can be conveniently and quickly quantified without resort to any troublesome pretreatments, and a biosensor. More particularly speaking, a method of quantifying a substrate in a sample by using an electrode system made of electrically conductive materials and a reaction reagent comprising at least a dehydrogenase, a coenzyme an electron mediator and a tetrazolium salt, which comprises performing an enzyme reaction and a redox reaction between the reaction reagent and the substrate in the sample, and detecting a formazan formed as the final reaction product by using the electrode system and a if biosensor with the use of the same are provided.
摘要翻译：定量底物的方法，通过该方法可以方便快速地定量各种样品中所含的底物，而不需要任何麻烦的预处理和生物传感器。更具体地说，通过使用由导电材料制成的电极系统和至少包含脱氢酶，辅酶，电子介体和四唑鎓盐的反应试剂来定量样品中的底物的方法，其包括进行酶反应和提供样品中反应试剂和底物之间的氧化还原反应，并且通过使用电极系统和如果使用其的生物传感器检测形成为最终反应产物的甲。。

9. 发明授权

US06448029B1 Method and reagent for quantitative determination of 1,5-anhydroglucitol 有权
标题翻译：用于定量测定1,5-脱水葡萄糖醇的方法和试剂
公开(公告)号：US06448029B1
公开(公告)日：2002-09-10
申请号：US09929038
申请日：2001-08-15
申请人： Sakae Tazoe , Akira Miike
发明人： Sakae Tazoe , Akira Miike
IPC分类号： C12Q132
CPC分类号： C12Q1/32 , C12Q1/54 , Y10S435/966 , Y10S435/975
摘要： The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要翻译：本发明提供了用于确定特定成分的简单方法，例如，含有葡萄糖的样品中的1,5-脱水葡萄糖醇（1,5-AG），以及可用于该方法的试剂和试剂盒。在一个实施方案中，提供了用于测定1,5-AG的方法，其包括将样品与将葡萄糖转化为果糖-1,6-二磷酸并将1,5-AG转化为1,5-AG的酶系统接触 -6-磷酸形成1,5-AG-6-磷酸，通过1,5-AG-6-磷酸脱氢酶的作用在氧化型辅酶存在下使样品中的1,5-AG-6-磷酸脱氢，并测定由脱氢反应形成的还原型辅酶的量。还提供了一种在该方法中有用的试剂和试剂盒。

10. 发明授权

US06387648B1 Method for adjusting and disinfecting liquids 失效
标题翻译：液体调节和消毒方法
公开(公告)号：US06387648B1
公开(公告)日：2002-05-14
申请号：US09508964
申请日：2000-07-17
申请人： Yves Levi , Danièle Touati , Sam Dukan
发明人： Yves Levi , Danièle Touati , Sam Dukan
IPC分类号： C12Q132
CPC分类号： C12Q1/04 , C12Q1/22
摘要： A Method for regulating the disinfection of a liquid includes a number of stages. At one stage of the said disinfection (stage 2), the activity of at least one enzyme is measured by bringing the microorganisms which may be present in the liquid into contact with a substrate capable of revealing the activity of the enzyme, this enzymatic activity being referred to as specific activity. At another stage (stage 1), prior to stage 2, there occurs measuring the activity of the same enzymes. This activity being referred to as initial activity. The next stage involves translating, for each enzyme, the specific activity and initial activity, into levels of microorganisms surviving in the liquid at stage 2 of the disinfection by means of a reference system pre-established with the aid of a sample of the liquid collected at stage 1 and then exposed to increasing doses of disinfectant. Then there is the next stage for adjusting, as a function of the level of surviving microorganisms, the nature and/or doses of physical or chemical agent(s) used for disinfection.
摘要翻译：调节液体消毒的方法包括多个阶段。在所述消毒的一个阶段（阶段2）中，通过使可能存在于液体中的微生物与能够显示酶的活性的底物接触来测量至少一种酶的活性，该酶活性为简称为具体活动。在另一个阶段（阶段1），在阶段2之前，测量相同酶的活性。此活动被称为初始活动。下一阶段涉及将每种酶的比活性和初始活性转化为在消毒阶段2中在液体中存活的微生物水平，借助于借助于采集的液体样品预先建立的参考系统在第1阶段，然后暴露于增加剂量的消毒剂。那么下一个阶段是作为微生物存活水平的函数调整用于消毒的物理或化学试剂的性质和/或剂量。

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