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1. 发明申请

US20150284743A1 METHODS FOR THE ENZYMATIC PRODUCTION OF ISOPRENE FROM ISOPRENOL 审中-公开
标题翻译：从异丙酚生产异丙苯的方法
公开(公告)号：US20150284743A1
公开(公告)日：2015-10-08
申请号：US14442005
申请日：2013-11-08
申请人： GLOBAL BIOENERGIES , SCIENTIST OF FORTUNE S.A.
发明人： Philippe Marliere , Maria Anissimova
IPC分类号： C12P5/00 , C12N9/10 , C12N9/90 , C12N9/12 , C12N9/88
CPC分类号： C12P5/007 , C12N9/1205 , C12N9/13 , C12N9/88 , C12N9/90 , C12Y207/0105 , C12Y208/02 , C12Y402/03014 , C12Y402/03015 , C12Y402/03046 , C12Y402/03047 , C12Y503/03002
摘要： Described are methods for the enzymatic production of isoprene which allow to produce isoprene from isoprenol and microorganisms which have been genetically modified so as to be able to produce isoprene from isoprenol.Also described are enzyme combinations which allow to convert isoprenol into isoprene as well as (micro)organisms which express such enzyme combinations.
摘要翻译：描述了用于产生异戊二烯的方法，其允许从异戊二烯醇产生异戊二烯和已被遗传修饰以便能够从异戊二烯醇产生异戊二烯的微生物。还描述了允许将异戊二烯醇转化成异戊二烯以及表达这种酶组合的（微）生物体的酶组合。

2. 发明公开

US20240076699A1 BIOSYNTHESIS OF SUBSTITUTED COMPOUNDS AND CANNABINOIDS 审中-公开
公开(公告)号：US20240076699A1
公开(公告)日：2024-03-07
申请号：US18227719
申请日：2023-07-28
申请人： Invizyne Technologies, Inc.
发明人： Tyler P. Korman , Bastian Vogeli , Andrew Lingard , Mohammad Hayat
IPC分类号： C12P7/22 , C12N9/10 , C12N9/12
CPC分类号： C12P7/22 , C12N9/1085 , C12N9/1205 , C12Y205/0101 , C12Y207/0105 , C12Y207/01127
摘要： Systems and methods for prenylation and recombinant pathways for the production of compounds, cannabinoids, cannabinoid precursors, and other prenylated chemicals in a cell free system. The present invention further includes enzymes and recombinant microorganisms that catalyze the reaction. The compounds, cannabinoids, cannabinoid precursors, and other prenylated chemicals are operable to be substituted with at least one deuterium, at least one tritium, at least one halogen, at least one hydroxyl group, and/or at least one additional isotope.

3. 发明授权

US12084704B2 Methods for producing isobutene from 3-methylcrotonic acid 有权
公开(公告)号：US12084704B2
公开(公告)日：2024-09-10
申请号：US16612065
申请日：2018-04-19
申请人： Global Bioenergies
发明人： Romain Chayot , Mathieu Allard , Maria Anissimova
IPC分类号： C12P5/02
CPC分类号： C12P5/026 , C12Y205/01 , C12Y207/0105 , C12Y207/04026 , C12Y207/06002 , C12Y207/06003 , C12Y401/01 , C12Y503/03002
摘要： Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically by: (i) the enzymatic conversion of dimethylallyl pyrophosphate (DMAPP) into said DMAP; or (ii) a single enzymatic step in which prenol is directly enzymatically converted into said DMAP; or (iii) two enzymatic steps comprising: first enzymatically converting DMAPP into prenol; and then enzymatically converting the thus obtained prenol into said DMAP; or (iv) the enzymatic conversion of isopentenyl monophosphate (IMP) into said DMAP, or by a combination of any one of (i) to (iv). Moreover, described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically by: (v) the enzymatic conversion of isopentenyl pyrophosphate (IPP) into said DMAPP; or (vi) the enzymatic conversion of dimethylallyl phosphate (DMAP) into said DMAPP; or (vii) the enzymatic conversion of prenol into said DMAPP; (viii) or by a combination of any one of (v) to (vii). Moreover, described are methods for providing said flavin cofactor enzymatically by the enzymatic conversion of riboflavin into flavin mononucleotide (FMN).

4. 发明公开

US20240318211A1 RECOMBINANT PRODUCTION OF CANNABINOIDS 审中-公开
公开(公告)号：US20240318211A1
公开(公告)日：2024-09-26
申请号：US18587332
申请日：2024-02-26
申请人： 1000128725 Ontario Inc.
发明人： Hamed Hasheminejad , Mahdi Shahsavand
IPC分类号： C12P17/06 , C12N9/10 , C12N9/12 , C12N9/90 , C12P7/02
CPC分类号： C12P17/06 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/90 , C12P7/02 , C12Y205/01001 , C12Y205/01039 , C12Y207/0105 , C12Y207/04026 , C12Y503/03002
摘要： Ways of making and using a recombinant organism configured to produce a cannabinoid are provided. The recombinant organism can include a eukaryotic microorganism expressing a recombinant construct including a geranyl diphosphate synthase (GPPS2), an isopentenyl diphosphate isomerase (IDI), an isopentenyl phosphate kinase (IPK), and a 5-(hydroxyethyl)-methyl thiazole kinase (ThiM). A cannabinoid can be produced by a process that includes growing the recombinant organism configured to produce the cannabinoid in a growth medium and separating the cannabinoid from the recombinant organism and the growth medium. A biosynthetic system for producing a cannabinoid is provided that includes a bioreactor, the recombinant organism configured to produce the cannabinoid, and a growth medium for the recombinant organism.

5. 发明公开

EP2920314A1 PROCESS FOR THE ENZYMATIC PREPARATION OF ISOPRENE FROM ISOPRENOL 审中-公开
标题翻译：方法异戊二烯OFF异戊烯醇酶生产
公开(公告)号：EP2920314A1
公开(公告)日：2015-09-23
申请号：EP13799494.3
申请日：2013-11-08
申请人： Global Bioenergies , Scientist of Fortune S.A.
发明人： MARLIÈRE, Philippe , ANISSIMOVA, Maria
IPC分类号： C12P5/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90
CPC分类号： C12P5/007 , C12N9/1205 , C12N9/13 , C12N9/88 , C12N9/90 , C12Y207/0105 , C12Y208/02 , C12Y402/03014 , C12Y402/03015 , C12Y402/03046 , C12Y402/03047 , C12Y503/03002
摘要： Described are methods for the enzymatic production of isoprene which allow to produce isoprene from isoprenol and microorganisms which have been genetically modified so as to be able to produce isoprene from isoprenol. Also described are enzyme combinations which allow to convert isoprenol into isoprene as well as (micro)organisms which express such enzyme combinations.

6. 发明申请

WO2014076016A1 PROCESS FOR THE ENZYMATIC PREPARATION OF ISOPRENE FROM ISOPRENOL 审中-公开
标题翻译：异丙苯的制备方法
公开(公告)号：WO2014076016A1
公开(公告)日：2014-05-22
申请号：PCT/EP2013/073425
申请日：2013-11-08
申请人： GLOBAL BIOENERGIES , SCIENTIST OF FORTUNE S.A.
发明人： MARLIÈRE, Philippe , ANISSIMOVA, Maria
IPC分类号： C12P5/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90
CPC分类号： C12P5/007 , C12N9/1205 , C12N9/13 , C12N9/88 , C12N9/90 , C12Y207/0105 , C12Y208/02 , C12Y402/03014 , C12Y402/03015 , C12Y402/03046 , C12Y402/03047 , C12Y503/03002
摘要： Described are methods for the enzymatic production of isoprene which allow to produce isoprene from isoprenol and microorganisms which have been genetically modified so as to be able to produce isoprene from isoprenol. Also described are enzyme combinations which allow to convert isoprenol into isoprene as well as (micro)organisms which express such enzyme combinations.
摘要翻译：描述了用于产生异戊二烯的方法，其允许从异戊二烯醇产生异戊二烯和已经被遗传修饰以便能够从异戊二烯醇产生异戊二烯的微生物。还描述了允许将异戊二烯醇转化成异戊二烯以及表达这种酶组合的（微）生物体的酶组合。

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