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    • 2. 发明申请
    • METHOD OF AMPLIFYING AND DETECTING TARGET NUCLEIC ACID SEQUENCE BY USING THERMOSTABLE ENZYMES
    • 通过使用可热稳定酶的方法来放大和检测目标核酸序列
    • WO1995015399A1
    • 1995-06-08
    • PCT/JP1994002025
    • 1994-12-01
    • TOYO BOSEKI KABUSHIKI KAISHATAKARADA, YutakaINOUE, HiroakiSHIBATA, ShujiKAWAMURA, Yoshihisa
    • TOYO BOSEKI KABUSHIKI KAISHA
    • C12Q01/68
    • C12Q1/6865C12Q2527/101
    • A method of amplifying a specified nucleic acid sequence, which permits an amplification reduced in nonspecific hybridization and increased in specificity. The invention also provides a reagent which does not suffer any activity drop during supply and preservation thereof and is highly stable. Thermostable enzymes are used as the RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, DNA-dependent RNA polymerase and ribonuclease H, all of which are necessary for the replicative RNA-base amplification system. It is desirable to use combinedly a thermostable DNA-dependent RNA polymerase and a thermostable enzyme originating in Thermus thermophilus which comprises all of the RNA-dependent DNA polymerase, DNA-dependent DNA polymerase and ribonuclease H. The use of the thermostable enzymes according to the above method serves to prevent the enzymes from being deactivated and enables the amplification without the necessity for sequentially adding the enzymes.
    • 扩增指定核酸序列的方法,其允许在非特异性杂交中减少扩增并增加特异性。 本发明还提供一种在其供给和保存期间不会发生任何活性下降的试剂,并且高度稳定。 热稳定酶用作RNA依赖性DNA聚合酶,DNA依赖性DNA聚合酶,DNA依赖性RNA聚合酶和核糖核酸酶H,这些都是复制RNA碱基扩增系统所必需的。 期望组合使用热稳定的DNA依赖性RNA聚合酶和源自嗜热栖热菌的热稳定酶,其包含所有RNA依赖性DNA聚合酶,DNA依赖性DNA聚合酶和核糖核酸酶H.使用 的根据上述方法的热稳定酶用于防止酶失活并且能够进行扩增,而不需要依次加入酶。
    • 3. 发明申请
    • WHOLLY AROMATIC POLYESTER
    • 纯粹的芳香聚酯
    • WO1989005323A1
    • 1989-06-15
    • PCT/JP1988001247
    • 1988-12-09
    • TOYO BOSEKI KABUSHIKI KAISHAKIMURA, KunioKATO, Yasuo
    • TOYO BOSEKI KABUSHIKI KAISHA
    • C08G63/60
    • C08G63/605
    • A wholly aromatic polyester composed of as constituting units: (I) p-hydroxybenzoic acid and/or m-hydroxybenzoic acid residues; (II) hydroquinone residues; (III) aromatic diol residues represented by -O-Ar-O- (wherein Ar represents a 1,1-isopropylidene-4,4'-diphenylene group, a 2,6-naphthylene group, 4,4'-biphenylene group, a 1,3-phenylene group, etc.); and (IV) isophthalic acid residues (provided that, when (I) contains both p-hydroxybenzoic acid and m-hydroxybenzoic acid residues, (III) may not be contained), which has a melting point up to about 325 DEG C, a melt viscosity of 50 to 100,000 P, and which, when melted, is converted from the crystalline form to an optically isotropic melt. This polyester permits melt molding with ease and without uniaxial orientation and anisotropy in mechanical properties and can afford moldings and films.
    • 作为构成单元构成的全芳香族聚酯:(I)对羟基苯甲酸和/或间羟基苯甲酸残基; (II)氢醌残基; (III)由-O-Ar-O-(其中Ar表示1,1-异亚丙基-4,4'-二亚苯基,2,6-亚萘基,4,4'-亚联苯基, 1,3-亚苯基等); 和(IV)间苯二甲酸残基(条件是当(I)含有对羟基苯甲酸和间羟基苯甲酸残基时,(III)可以不含),其熔点高达约325℃, 熔融粘度为50〜100,000P,当熔融时,从结晶形式转变为光学各向同性熔体。 该聚酯可以容易地进行熔融模塑,并且不具有单轴取向和机械性能的各向异性,并且可以提供模制品和薄膜。
    • 5. 发明申请
    • RESIN-CHROMATE COMPOSITION AND SURFACE-TREATED METAL SHEET
    • 树脂 - 铬酸盐组合物和表面处理金属片
    • WO1997007261A1
    • 1997-02-27
    • PCT/JP1996002270
    • 1996-08-09
    • NIPPON STEEL CORPORATIONTOYO BOSEKI KABUSHIKI KAISHANAKAZAWA, MakotoIZAKI, TeruakiHAYASHI, KimitakaSUZUKI, ShinichiMIYAUCHI, YujiroYOSHIDA, KengoODASHIMA, HisaoTAKAHASHI, TomomiSHIBABUKI, SyujiFUJIOKA, YujiYAMAZAKI, MakotoTADOKORO, Kenichiro
    • NIPPON STEEL CORPORATIONTOYO BOSEKI KABUSHIKI KAISHA
    • C23C22/28
    • C09D5/08B05D7/14C23C22/28C23C22/33
    • A resin-chromate composition mainly comprising an emulsion of (a) an organic polymer composed of ethylenically unsaturated compounds in an aqueous medium, (b) a water-soluble chromium compound, and (c) a mineral acid, characterized in that the content of (a1) ethylenically unsaturated carboxylic acid components constituting the organic polymer (a) is more than 10 wt.% but below 30 wt.% based on the weight (in terms of solid matter) of the organic polymer (a), that of (a2) ethylenically unsaturated hydroxylated compound components constituting the polymer (a) is 0 wt.% or above but below 3 wt.% based thereon, and the balance is composed of (a3) ethylenically unsaturated compound components bearing neither carboxyl nor hydroxyl groups and that the content of components forming C3-C7 monocarboxylic acids in an aqueous solution of chromic acid and/or a chromate coat is 20 wt.% or below based on the weight (in terms of solid matter) of the organic polymer; and a metal sheet surface-treated with this composition.The above chromate composition is excellent in bath stability and smelling characteristics and the metal sheet surface-treated with the composition does not produce any putrid smell and is excellent in corrosion resistance, prevention of leaching of chromium, resistance to alkali, adhesion of paint, and appearance and quality.
    • 一种树脂铬酸盐组合物,主要包含(a)在水性介质中由烯属不饱和化合物构成的有机聚合物的乳液,(b)水溶性铬化合物和(c)无机酸,其特征在于, 构成有机聚合物(a)的(a1)烯键式不饱和羧酸成分,相对于有机聚合物(a)的重量(以固体成分计),(a)的重量(以固体成分计)为10重量%以下,低于30重量% a2)构成聚合物(a)的烯键式不饱和羟基化合物组分相对于其为0重量%以上但低于3重量%,余量由(a3)不具有羧基和羟基的烯键式不饱和化合物组分构成, 基于有机聚合物的重量(以固体物质计),在铬酸和/或铬酸盐涂层的水溶液中形成C3-C7单羧酸的成分的含量为20重量%以下。 上述铬酸盐组合物的浴稳定性和气味特性优异,用组合物表面处理的金属片不产生腐臭,耐腐蚀性优异,防止浸出的浸出性 铬,耐碱性,涂料附着力,外观和质量。
    • 10. 发明申请
    • ORIGINAL PLATE FOR DAMPENING WATER-FREE LITHOGRAPHY AND PROCESS FOR MAKING PRINTING PLATE USING SAME
    • 用于防止无水平面的原始板和使用其制造印刷板的工艺
    • WO1983002175A1
    • 1983-06-23
    • PCT/JP1981000398
    • 1981-12-18
    • TOYO BOSEKI KABUSHIKI KAISHAWADA, MinoruTOMITA, AkiraNISHIWAKI, ToshikazuETOH, KuniomiTANAKA, ShinichiFUGIMURA, ToshiakiIGUCHI, Mitsuo
    • TOYO BOSEKI KABUSHIKI KAISHA
    • G03F07/02
    • G03F7/0752
    • An original plate for dampening water-free lithography comprising a laminate of a support (1), an optional adhesive substratum (2), a photo-sensitive layer (3), an ink-repelling layer consisting of a metal atom (6) having a principal quantum number of at least 3 for increasing a bonding force to said photosensitive layer (3) and an organo-polysiloxane compound (7) or a perfluoroalkyl compound (7) having a functional group bound to said metal atom (6), and optional cover film (5), and a process for making a dampening water-free lithographic printing plate, which comprises delaminating cover film (5), if present, bringing a negative or positive film into close contact with said original plate, exposing with actinic ray, and developing with a solvent, thus forming ink-accepting portions (11) from which said photosensitive layer an ink-repelling layer have been dissolved away and ink-repelling portions (12) where said photosensitive layer and said ink-repelling layer remain undissolved.
    • 一种用于抑制无水平版印刷的原版,包括支撑体(1),任选的粘合剂基底(2),光敏层(3),由金属原子(6)组成的防油层 用于增加对所述感光层(3)的结合力的至少3的主量子数和具有与所述金属原子(6)结合的官能团的有机聚硅氧烷化合物(7)或全氟烷基化合物(7),以及 可选的覆盖膜(5)以及制造无水润版印刷版的方法,其包括使覆盖膜(5)分层(如果存在),使负片或正片与所述原版板紧密接触,用光化剂曝光 并且用溶剂显影,从而形成吸墨部分(11),所述感光层已经从中去除了防墨层,并且所述感光层和所述防墨层保留的防墨部分(12) 溶解。