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1. 发明申请

WO2019212615A1 METHODS OF AMPLIFYING NUCLEIC ACIDS AND COMPOSITIONS AND KITS FOR PRACTICING THE SAME 审中-公开
公开(公告)号：WO2019212615A1
公开(公告)日：2019-11-07
申请号：PCT/US2019/016988
申请日：2019-02-07
申请人： TAKARA BIO USA, INC.
发明人： TORI, Kazuo
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
摘要： Methods of amplifying nucleic acids in a sample are provided. Aspects of the methods include: a) fragmenting nucleic acids in the sample to produce a fragmented nucleic acid sample; b) contacting the fragmented nucleic acid sample with a cDNA synthesis primer comprising a RNA origination domain under cDNA synthesis conditions to produce a product nucleic acid composition; and c) amplifying the product nucleic acid composition. Compositions and kits for use in performing the methods are also provided.

2. 发明申请

WO2017214417A1 METHODS AND COMPOSITIONS EMPLOYING BLOCKED PRIMERS 审中-公开
标题翻译：使用阻塞的引物的方法和组合物
公开(公告)号：WO2017214417A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/036583
申请日：2017-06-08
申请人： TAKARA BIO USA, INC.
发明人： SCHAAL, Thomas D. , DUNNE, Jude , SRINIVASAN, Maithreyan , MIR, Alain
IPC分类号： C12P19/34 , C12Q1/68 , G01N15/06
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/6844 , C12Q2525/186
摘要： The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.
摘要翻译：本公开提供了使用封闭引物的方法，组合物和系统。本公开的方面包括提供包含来自单细胞的封闭引物和模板核酸组分的封闭引物反应混合物; 解封封闭的引物以产生活性引物反应混合物并使活化的引物反应混合物经历引物延伸条件，如核酸扩增条件。

3. 发明申请

WO2017205687A1 CONTACT DISPENSING OF CELLS INTO MULTI-WELL DEVICES 审中-公开
标题翻译：将电池分配到多个设备中
公开(公告)号：WO2017205687A1
公开(公告)日：2017-11-30
申请号：PCT/US2017/034568
申请日：2017-05-25
申请人： TAKARA BIO USA, INC.
发明人： HUSAIN, Syed A. , GRISWOLD, Bradley L. , SRINIVASAN, Maithreyan
IPC分类号： B01L3/00 , G01N1/40 , G01N15/10
摘要： The present disclosure provides methods, devices, assemblies, and systems for contact dispensing of cells into multi-well devices. For example, provided herein are systems and methods for contact dispensing a dispense volume into a plurality of wells of a multi-well device, where the multi-well device has a hydrophobic top surface (e.g., a contact agent greater than 140 degrees, including greater than 165 degrees) and wells which have a relatively hydrophilic well surface (e.g., contact angle of 65-80 degrees). In some embodiments, a dispensing tip has a hanging drop of liquid (e.g., containing a cell) that is touched off onto the hydrophobic top surface of the multi-well device such that is repelled by the top surface and collected into, and attracted by, the relatively hydrophilic surface of the wells.
摘要翻译：本公开提供了用于将细胞接触分配到多孔装置中的方法，装置，组件和系统。例如，本文提供用于将分配体积接触分配到多孔装置的多个孔中的系统和方法，其中多孔装置具有疏水顶面（例如，大于140度的接触剂，包括大于165度）和具有相对亲水井表面（例如，65-80度的接触角）的井。在一些实施例中，分配尖端具有悬挂在多孔装置的疏水顶表面上的液滴（例如，包含细胞）的悬滴，从而被顶表面排斥并被收集到并被吸引，这些孔相对亲水的表面。

4. 发明申请

WO2020131383A1 NORMALIZATION OF NUCLEIC ACID SAMPLES AND COMPOSITIONS FOR USE IN THE SAME 审中-公开
公开(公告)号：WO2020131383A1
公开(公告)日：2020-06-25
申请号：PCT/US2019/064477
申请日：2019-12-04
申请人： TAKARA BIO USA, INC.
发明人： TORI, Kazuo
IPC分类号： C12Q1/68 , C40B40/06 , C07H21/04 , C07H21/00
摘要： Methods of normalizing two or more nucleic acid samples are provided. Aspects of the methods include contacting each of the two or more nucleic acid samples with a limiting amount of a target binding moiety that specifically binds to a common target in each of the two or more nucleic acid samples to produce binding complexes in each of the two or more nucleic acid samples; and separating the binding complexes from unbound nucleic acids in each of the two or more nucleic acid samples to normalize the two or more nucleic acid samples. Compositions and kits for use in performing the methods are also provided.

5. 发明申请

WO2018089550A1 METHODS OF PRODUCING AMPLIFIED DOUBLE STRANDED DEOXYRIBONUCLEIC ACIDS AND COMPOSITIONS AND KITS FOR USE THEREIN 审中-公开
标题翻译：扩增双链脱氧核糖核酸的制备方法及其用于其中的组合物和试剂盒
公开(公告)号：WO2018089550A1
公开(公告)日：2018-05-17
申请号：PCT/US2017/060717
申请日：2017-11-08
申请人： TAKARA BIO USA, INC.
发明人： TORI, Kazuo , BOSTICK, Magnolia , FARMER, Andrew
IPC分类号： C12P19/34 , C12Q1/6806
摘要： Methods of producing an amplified double stranded deoxyribonucleic acid (dsDNA) from a nucleic acid sample are provided. Aspects of the methods include amplifying using a single product nucleic acid primer and a template switch oligonucleotide to produce an amplified dsDNA product. Compositions and kits for use in performing the methods are also provided.
摘要翻译：提供了从核酸样品产生扩增的双链脱氧核糖核酸（dsDNA）的方法。方法的各方面包括使用单一产物核酸引物和模板转换寡核苷酸进行扩增以产生扩增的dsDNA产物。还提供了用于执行这些方法的组合物和试剂盒。

6. 发明申请

WO2017048993A1 METHODS FOR PREPARING A NEXT GENERATION SEQUENCING (NGS) LIBRARY FROM A RIBONUCLEIC ACID (RNA) SAMPLE AND COMPOSITIONS FOR PRACTICING THE SAME 审中-公开
标题翻译：用于制备来自核糖核酸（RNA）样品的下一个产生序列（NGS）图谱的方法和用于实施其的组合物
公开(公告)号：WO2017048993A1
公开(公告)日：2017-03-23
申请号：PCT/US2016/051989
申请日：2016-09-15
申请人： TAKARA BIO USA, INC.
发明人： CHANG, Cynthia , BOSTICK, Magnolia
IPC分类号： G06F19/10 , G06F19/20 , G06F19/22 , C40B40/06 , C40B40/08
CPC分类号： C12N15/1093 , C12N15/1096 , C12Q2525/191
摘要： Methods of preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample are provided. Aspects of the methods include combining the RNA sample with a first strand cDNA primer and a template switch oligonucleotide under first strand cDNA synthesis conditions, where one of the first strand cDNA primer and the template switch oligonucleotide includes a first post-tagmentation amplification primer binding domain. The resultant product is subjected to amplification conditions sufficient to produce a double stranded cDNA, which is then tagmented with a transposome that includes a second post-tagmentation amplification primer binding domain. The tagmented sample is then subjected to amplificationconditions using first and second post-tagmentation amplification primers that include sequencing platform adapter constructs to produce a NGS library. Aspects of the invention further include compositions produced by the methods and kits that find use in practicing the methods.
摘要翻译：提供了从核糖核酸（RNA）样品制备下一代测序（NGS）文库的方法。所述方法的方面包括在第一链cDNA合成条件下将RNA样品与第一链cDNA引物和模板切换寡核苷酸组合，其中第一链cDNA引物和模板切换寡核苷酸之一包含第一后标记扩增引物结合结构域。对所得产物进行足以产生双链cDNA的扩增条件，然后用包含第二后标记扩增引物结合结构域的转座子进行标记。然后使用包括测序平台适配器构建体的第一和第二后标记扩增引物对标记的样品进行扩增条件以产生NGS文库。本发明的方面还包括通过用于实施该方法的方法和试剂盒产生的组合物。

7. 发明申请

WO2019040788A1 METHODS OF PRODUCING NUCLEIC ACIDS USING OLIGONUCLEOTIDES MODIFIED BY A STIMULUS 审中-公开
公开(公告)号：WO2019040788A1
公开(公告)日：2019-02-28
申请号：PCT/US2018/047816
申请日：2018-08-23
申请人： TAKARA BIO USA, INC.
发明人： TORI, Kazuo
IPC分类号： C12N15/11 , C12N15/67 , C12P19/34
摘要： Provided are methods of producing product nucleic acids involving the use of oligonucleotides that are modified by the application of a stimulus. Aspects of such methods may include producing product nucleic acids using de-activatable oligonucleotides that are deactivated by a de-activating stimulus, as well as methods that may include producing product nucleic acids using activatable oligonucleotides that are activated by an activating stimulus and de-activatable oligonucleotides that are deactivated by a de-activating stimulus. Also provided are kits, compositions and devices that include de-activatable oligonucleotides or activatable and de-activatable oligonucleotides, e.g., for use in performing the methods as described herein.

8. 发明申请

WO2022076196A1 METHODS AND COMPOSITIONS FOR PREPARING NUCLEIC ACIDS FOR GENETIC ANALYSIS 审中-公开
公开(公告)号：WO2022076196A1
公开(公告)日：2022-04-14
申请号：PCT/US2021/052337
申请日：2021-09-28
申请人： TAKARA BIO USA, INC.
发明人： MEYERS, Jacob , LALIBERTÉ, Julie Catherine , MARTIN, Patrick Kevin
IPC分类号： C12Q1/6806 , C12Q1/686 , C12Q1/6851 , C12Q1/6844 , C12N15/10
摘要： The present disclosure provides methods and compositions for preparing nucleic acid molecules using a novel, single-tube workflow that allows users to carry out the isolation of nucleic acids from a sample and perform a nucleic acid assay using the isolated nucleic acids in a single tube or vessel. As such, the methods disclosed herein can be used with low quality and/or small quantity of nucleic acid samples. Further, the disclosed methods offer similar or improved concordance rates in addition to improved signal to noise ratio when compared to the existing methods used for genetic analysis. Thus, making the methods disclosed herein suitable for clinical applications such as infectious disease diagnosis, pre-implantation genetic testing, prenatal or cancer diagnosis.

9. 发明申请

WO2018031588A1 NUCLEIC ACID ADAPTORS WITH MOLECULAR IDENTIFICATION SEQUENCES AND USE THEREOF 审中-公开
标题翻译：具有分子鉴定序列的核酸适配子及其用途
公开(公告)号：WO2018031588A1
公开(公告)日：2018-02-15
申请号：PCT/US2017/045976
申请日：2017-08-08
申请人： TAKARA BIO USA, INC. , SUN, Fang , GORYUNOV, Dmitry , CHARIZANIS, Konstantinos , LANGMORE, John , KAMBEROV, Emmanuel
发明人： SUN, Fang , GORYUNOV, Dmitry , CHARIZANIS, Konstantinos , LANGMORE, John , KAMBEROV, Emmanuel
IPC分类号： C12N15/09 , C12Q1/68
摘要： Provided herein are double-stranded nucleic acid adaptors comprising molecular identification sequences. Further provided are methods of using the double-stranded nucleic acid adaptors for generating nucleic acid libraries, e.g., for amplification and sequencing.
摘要翻译：本文提供了包含分子鉴定序列的双链核酸衔接子。还提供了使用双链核酸衔接子产生核酸文库的方法，例如用于扩增和测序。

10. 发明申请

WO2018017892A1 MULTI-Z IMAGING AND DISPENSING WITH MULTI-WELL DEVICES 审中-公开
标题翻译：多Z成像和多井设备分配
公开(公告)号：WO2018017892A1
公开(公告)日：2018-01-25
申请号：PCT/US2017/043169
申请日：2017-07-20
申请人： TAKARA BIO USA, INC.
发明人： LIN, Chun-Wah , HUBSCHLE, Hermann , ESPINOZA VALLEJOS, Patricio A. , HUSAIN, Syed A.
IPC分类号： B01J19/00 , C40B60/14 , G01N21/25 , G01N21/64 , G01N21/76 , G01N35/02
摘要： Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.
摘要翻译：提供了用于分配到多井装置的井中并且从多个Z-平面对这些井进行成像的方法，装置，组件和系统。本方法和系统的多Z成像可以允许检测包含期望数量的细胞的多孔装置的孔。还提供了用于处理通过使用多Z成像识别的多孔装置的含细胞孔的方法，装置，组件和系统。

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