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    • 1. 发明申请
    • EFFECT OF TREATMENT WITH 4,5-DIHYDROXY- 2-CYCLOPENTEN-1-ONE (DHCP) ON GENE EXPRESSION AND QUORUM-SENSING IN BACTERIA
    • 用4,5-二羟基-2-环丙-1-酮(DHCP)处理细菌对细菌的基因表达和定量感测的影响
    • WO2003077844A2
    • 2003-09-25
    • PCT/US2003/007081
    • 2003-03-07
    • TAKARA BIO, INC.PHADTARE, SangitaKATO, IkunoshinINOUYE, Masayori
    • PHADTARE, SangitaKATO, IkunoshinINOUYE, Masayori
    • A61K
    • C12P21/00C12Q1/6837C12Q1/689C12Q2600/158
    • DHCP (4,5-dihydroxy-2-cyclopenten-1-one) has been previously shown to have antibacterial activity against Escherichia coli . Global transcriptional pattern of E . coli was analyzed in response to DHCP by DNA microarray. It is now shown that DHCP has widespread effects in E . coli , affecting genes encoding proteins involved in general metabolism and cell membrane synthesis and functions. In addition, rpoS and RpoS-regulated genes responding to various stresses are upregulated. DHCP is also shown to inhibit AI-2, an autoinducer involved in interspecies quorum-sensing, and the genes comprising quorum-regulated processes such as virulence, motility and outer membrane functions are downregulated by DHCP treatment. In addition, cysK which is a known quorum-sensing gene working in an alternate pathway(s) in E . coli increases considerably in response to DHCP. These results suggest that DHCP regulates the switching on/off of the different quorum-sensing circuits in E . coli .
    • 之前已经显示DHCP(4,5-二羟基-2-环戊烯-1-酮)对大肠杆菌具有抗菌活性。 通过DNA微阵列分析响应于DHCP的大肠杆菌的全局转录模式。 现在显示,DHCP在大肠杆菌中具有广泛的作用,影响编码参与一般代谢和细胞膜合成和功能的蛋白质的基因。 此外,rpoS和RpoS调控的各种应激反应的基因被上调。 还显示DHCP可以抑制AI-2,一种涉及种间群体感应的自动诱导剂,包括毒力调节过程如毒力,运动性和外膜功能在内的基因被DHCP处理下调。 此外,作为在大肠杆菌中的替代途径中工作的已知群体感应基因的cysK响应于DHCP而显着增加。 这些结果表明,DHCP调节大肠杆菌不同群体感应电路的开/关。