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    • 4. 发明公开
    • Method and apparatus for determining the volume and index of refraction of particles
    • Methode und Vorrichtung zur Bestimmung des Volumens und des Brechungsindexes von Teilchen。
    • EP0140616A1
    • 1985-05-08
    • EP84306942.8
    • 1984-10-11
    • TECHNICON INSTRUMENTS CORPORATION (a New York corporation)
    • Tycko, Daniel H.
    • G01N21/47
    • G01N15/1459G01N15/1012G01N21/41G01N2015/0073G01N2015/0238G01N2015/1018G01N2021/4707
    • The volume and/or index of refraction of a particle are determined by passing the particle through a beam of light directed along an optical path to produce at least one forward light scattering pattern;
      detecting and measuring the intensity of light at least within a selected portion of said at least one scattering pattern to generate at least a first signal and a second signal; and
      determining the volume and index of refraction of said particle from the magnitudes of said first and second signals. Apparatus for this purpose comprises means (26) for directing a light beam along an optical path,
      means (16) for passing said particle through said light beam to develop at least one forward light scattering pattern,
      means (42, 48) for detecting and measuring the intensity of light at least within a selected portion of said at least one scattering pattern and for generating a pair of first and second signals, and
      means responsive to said detecting and measuring means (42, 48) for determining and reporting the volume and/or the index of refraction of said particle from the magnitudes of said first and second signals. The method and apparatus are particularly useful for red blood cell analyses.
    • 颗粒的体积和/或折射率通过使颗粒通过沿着光路指向的光束来产生至少一个正向光散射图案来确定; ...至少在所选择的光束内检测和测量光的强度 所述至少一个散射图案的部分以产生至少第一信号和第二信号; 以及...从所述第一和第二信号的幅度确定所述粒子的体积和折射率。 用于此目的的装置包括用于沿光路引导光束的装置(26),用于使所述颗粒通过所述光束以形成至少一个正向光散射图案的装置(16),用于 至少在所述至少一个散射图案的选定部分内检测和测量光的强度,以及产生一对第一和第二信号,以及响应于所述检测和测量装置(42,48)来确定和报告的装置 所述粒子的体积和/或折射率与所述第一和第二信号的大小有关。 该方法和装置对于红细胞分析特别有用。