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1. 发明申请

WO2017172996A1 BIOMASS GENES 审中-公开
标题翻译：生物基因
公开(公告)号：WO2017172996A1
公开(公告)日：2017-10-05
申请号：PCT/US2017/024860
申请日：2017-03-29
申请人： SAPPHIRE ENERGY, INC.
发明人： YOHN, Christopher , HAMPTON, Eric , POON, Yan
IPC分类号： C12N15/82 , C12N1/12 , C12N1/20
CPC分类号： C12N15/8261 , C07K14/195 , C12N1/04 , C12N1/12 , C12N1/20 , Y02A40/146
摘要： Disclosed herein are polynucleotides and the polypeptides encoded thereby and their use to increase biomass production by photosynthetic organisms. Also provided are photosynthetic organisms transformed by such polynucleotides and expressing such polypeptides.
摘要翻译：本文公开了多核苷酸和由此编码的多肽及其用于增加光合生物体生物质产量的用途。还提供了由这种多核苷酸转化并表达这种多肽的光合生物。

2. 发明申请

WO2010051489A1 ANIMAL FEEDSTOCK COMPRISING GENETICALLY MODIFIED ALGAE 审中-公开
标题翻译：动物饲料包含基因改造的ALGAE
公开(公告)号：WO2010051489A1
公开(公告)日：2010-05-06
申请号：PCT/US2009/062843
申请日：2009-10-30
申请人： SAPPHIRE ENERGY, INC. , MENDEZ, Michael , BEHNKE, Craig , DAVIS, Linda
发明人： MENDEZ, Michael , BEHNKE, Craig , DAVIS, Linda
IPC分类号： C12N1/13 , A01H13/00 , A23K1/165 , A23L1/105
CPC分类号： C12N15/8243 , A23K20/158 , A23K20/168 , A23K50/10 , A23L7/104 , C10L1/04 , C12N1/12 , C12N1/22 , C12P5/007 , C12P9/00
摘要： The present disclosure provides methods for producing a photosynthetic organism, wherein the photosynthetic organism produces one or more enzymes that help degrade or digest a biomass, resulting in a more nutrient composition for use as animal feed. The invention also provides methods for producing a photosynthetic organism, wherein the photosynthetic organism produces one or more fatty acids or lipids that provide additional nutrient value. The fatty acids and lipids produced may also be used for the production of biofuel and the spent photosynthetic organisms can then be used as an animal feed. The invention also provides compositions of animal food comprising modified photosynthetic organisms.
摘要翻译：本公开提供了用于产生光合生物体的方法，其中光合生物体产生一种或多种有助于降解或消化生物质的酶，导致用作动物饲料的更多营养组合物。本发明还提供了生产光合生物的方法，其中光合生物产生一种或多种提供额外营养价值的脂肪酸或脂质。生产的脂肪酸和脂质也可用于生产生物燃料，然后用过的光合生物可以用作动物饲料。本发明还提供包含改良的光合生物的动物食品的组合物。

3. 发明申请

WO2017173005A1 MODIFIED RUBISCO LARGE SUBUNIT PROTEINS 审中-公开
标题翻译：修饰的RUBISCO大分子蛋白质
公开(公告)号：WO2017173005A1
公开(公告)日：2017-10-05
申请号：PCT/US2017/024870
申请日：2017-03-30
申请人： SAPPHIRE ENERGY, INC.
发明人： YOHN, Christopher , POON, Yan , SANTOS, Daniel , O'NEILL, Bryan
IPC分类号： C12N9/88 , C12N15/60 , C12N5/10
摘要： Provided herein are modified Rubisco large subunit proteins and nucleid acids encoding such proteins, as well as photosynthetic organism transformed with said nucleic acids and expressing said proteins. In certain embodiments, photosynthetic organisms containing said modified Rubisco large subunit proteins exhibit increased biomass production.
摘要翻译：本文提供了修饰的Rubisco大亚基蛋白质和编码这些蛋白质的核酸，以及用所述核酸转化并表达所述蛋白质的光合生物体。在某些实施方案中，含有所述经修饰的Rubisco大亚基蛋白质的光合生物显示出增加的生物量产量。

4. 发明申请

WO2013130406A1 LIPID AND GROWTH TRAIT GENES 审中-公开
标题翻译：脂肪和生长条件基因
公开(公告)号：WO2013130406A1
公开(公告)日：2013-09-06
申请号：PCT/US2013/027661
申请日：2013-02-25
申请人： SAPPHIRE ENERGY, INC. , YOHN, Christopher
发明人： YOHN, Christopher
IPC分类号： C12N15/31 , C12R1/89
CPC分类号： C12N15/8247 , C07K14/405 , C12N9/00 , C12P7/6463
摘要： The present disclosure provides novel lipid and growth stress response target genes isolated from Chlamydomonas reinhardtii that when over expressed in an organism results in a change in the lipid profile, and/or lipid content, and/or growth of the organism. The present disclosure also describes organisms expressing the genes, and methods of using the novel genes to change the lipid content, lipid profile or growth of an organism.
摘要翻译：本公开提供从衣藻衣藻中分离的新型脂质和生长应激应答靶基因，当在生物体中过度表达导致脂质分布和/或脂质含量和/或生物体的变化时。本公开还描述了表达基因的生物体，以及使用新基因改变生物体的脂质含量，脂质分布或生长的方法。

5. 发明申请

WO2013023086A1 COMPOSITIONS OF MATTER COMPRISING EXTRACTED ALGAE OIL 审中-公开
标题翻译：包含提取的阿尔法油的成分组合物
公开(公告)号：WO2013023086A1
公开(公告)日：2013-02-14
申请号：PCT/US2012/050189
申请日：2012-08-09
申请人： SAPPHIRE ENERGY, INC. , ROUSSIS, Stilianos , CRANFORD, Richard
发明人： ROUSSIS, Stilianos , CRANFORD, Richard
IPC分类号： C11B1/10 , C11B3/12 , C10G3/00 , B01D11/02 , C12N1/26
CPC分类号： C10G3/00 , A23D9/00 , A23D9/007 , C10G3/42 , C10G3/50 , C10G2300/1014 , C12P7/6409 , C12P7/6436 , Y02P30/20
摘要： Crude algae oils produced by practical extraction techniques comprise a wide range of molecular species that can be characterized by advanced analytical techniques. The algae oils comprise a complex mixture of a large number of molecules having varying sizes and therefore varying boiling points, and comprise high nitrogen, oxygen, and fatty acid content, but low sulfur, saturated hydrocarbons, and triglyceride content. Hydrogen/carbon molar ratios are typically greater than 1.6. The wide range of molecular species in the crude algae oils, while unusual compared to conventional refinery feed stocks and vegetable oils, may be upgraded into fuels by conventional refining approaches such as hydrotreating and thermal treatment. Unusual behavior of the algae oils in thermal processing and/or hydrotreatment may provide a high quality product slate, with the flexibility to adjust the product slate due to enhanced cracking behavior exhibited by these algae oils.
摘要翻译：通过实际提取技术生产的粗藻油包括可以通过先进分析技术表征的广泛的分子种类。藻油包含大量具有不同大小并因此变化沸点的分子的复杂混合物，并且包含高的氮，氧和脂肪酸含量，但低硫，饱和烃和甘油三酯含量。氢/碳摩尔比通常大于1.6。与常规炼油原料和植物油相比，粗藻油中分子种类的广泛范围可能通过常规精炼方法如加氢处理和热处理而升级为燃料。藻类油在热加工和/或加氢处理中的不寻常行为可以提供高品质的产品板岩，具有由于这些藻油所表现出的增强的裂纹行为而调整产品板岩的灵活性。

6. 发明申请

WO2009158658A3 INDUCTION OF FLOCCULATION IN PHOTOSYNTHETIC ORGANISMS 审中-公开
标题翻译：诱导光合作用中的絮凝
公开(公告)号：WO2009158658A3
公开(公告)日：2010-04-29
申请号：PCT/US2009048929
申请日：2009-06-26
申请人： SAPPHIRE ENERGY INC , MENDEZ MICHAEL , BEHNKE CRAIG , POON YAN , LEE PHILIP
发明人： MENDEZ MICHAEL , BEHNKE CRAIG , POON YAN , LEE PHILIP
IPC分类号： C12N1/13 , C07K16/14 , C12N15/63
CPC分类号： C12N1/12 , C07K14/4726 , C07K16/16 , C12N1/02
摘要： The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.
摘要翻译：本发明提供了用于在光合生物体中产生絮凝部分的组合物和方法。光合生物被遗传修饰以影响絮凝部分的产生，分泌或两者。还提供了絮凝生物体的方法。

7. 发明申请

WO2009064777A3 PRODUCTION OF FC-FUSION POLYPEPTIDES IN EUKARYOTIC ALGAE 审中-公开
标题翻译： FC-融合多肽在真核生物中的生产
公开(公告)号：WO2009064777A3
公开(公告)日：2009-09-03
申请号：PCT/US2008083225
申请日：2008-11-12
申请人： SAPPHIRE ENERGY INC , HEIFETZ PETER B , FRANKLIN SCOTT E
发明人： HEIFETZ PETER B , FRANKLIN SCOTT E
IPC分类号： C07K19/00 , C07K16/00 , C12N1/00 , C12N1/12 , C12N15/62
CPC分类号： C12N15/8257 , C12N15/8258
摘要： Methods and compositions are disclosed to engineer plastids comprising heterologous genes encoding immuno-activating domains fused to an extracellular domain (ECD) of a receptor or surface glycoprotein, a growth factor or an enzyme and produced within a subcellular organelle, such as a chloroplast. The immuno-activating domains may include those regions of a protein capable of modulating the interaction between immune effector cells via proteins containing stereoselective binding domains and specific ligands, such as the Fc regions of antibodies. The present disclosure also demonstrates the utility of plants, including green algae, for the production of complex multi-domain fusion proteins as soluble bioactive therapeutic agents.
摘要翻译：公开了工程质粒，其包含编码与受体或表面糖蛋白的细胞外结构域（ECD），生长因子或酶融合并在亚细胞器例如叶绿体内产生的免疫激活结构域的异源基因。免疫激活结构域可以包括能够通过含有立体选择性结合结构域和特异性配体（例如抗体的Fc区）的蛋白质调节免疫效应细胞之间的相互作用的蛋白质的那些区域。本公开还证实了包括绿藻在内的植物用于生产复杂的多结构域融合蛋白作为可溶性生物活性治疗剂的用途。

8. 发明申请

WO2009039015A3 METHODS FOR REFINING HYDROCARBON FEEDSTOCKS 审中-公开
标题翻译：炼油方法
公开(公告)号：WO2009039015A3
公开(公告)日：2009-05-07
申请号：PCT/US2008075892
申请日：2008-09-10
申请人： SAPPHIRE ENERGY INC , ARAVANIS ALEX , PYLE JASON , PRICE GEOFFREY , CRUNKLETON DANIEL
发明人： ARAVANIS ALEX , PYLE JASON , PRICE GEOFFREY , CRUNKLETON DANIEL
IPC分类号： C10G11/00
CPC分类号： C07C4/06 , C10G3/49 , C10G2300/1014 , C10G2300/104 , C10G2300/1044 , C10G2300/1051 , C10G2300/1055 , C10G2300/305 , C10G2400/30 , Y02P30/20
摘要： Processes of converting to feedstocks comprising hydrocarbons to compositions comprising light hydrocarbon products are described herein. Also described are processes and methods of producing and refining compositions comprising terpenes from biomass that can be suitable as a fuel product.
摘要翻译：本文描述了将包含烃的原料转化为包含轻质烃产物的组合物的方法。还描述了生产和精制组合物的方法和方法，所述组合物包含可以适合作为燃料产物的来自生物质的萜烯。

9. 发明申请

WO2009039015A2 METHODS FOR REFINING HYDROCARBON FEEDSTOCKS 审中-公开
标题翻译：炼油烃类原料的方法
公开(公告)号：WO2009039015A2
公开(公告)日：2009-03-26
申请号：PCT/US2008/075892
申请日：2008-09-10
申请人： SAPPHIRE ENERGY, INC. , ARAVANIS, Alex , PYLE, Jason , PRICE, Geoffrey , CRUNKLETON, Daniel
发明人： ARAVANIS, Alex , PYLE, Jason , PRICE, Geoffrey , CRUNKLETON, Daniel
IPC分类号： C10G11/00 , C10L1/18
CPC分类号： C07C4/06 , C10G3/49 , C10G2300/1014 , C10G2300/104 , C10G2300/1044 , C10G2300/1051 , C10G2300/1055 , C10G2300/305 , C10G2400/30 , Y02P30/20
摘要： Processes of converting to feedstocks comprising hydrocarbons to compositions comprising light hydrocarbon products are described herein. Also described are processes and methods of producing and refining compositions comprising terpenes from biomass that can be suitable as a fuel product.
摘要翻译：本文描述了将包含烃的原料转化成包含轻质烃产物的组合物的方法。还描述了从适合作为燃料产品的生物质生产和精炼包含萜烯的组合物的方法和方法。

10. 发明申请

WO2014092993A1 HARVESTING ALGAE FROM SALINE WATER USING FLOCCULANTS 审中-公开
标题翻译：使用絮凝剂从海水中收集水分
公开(公告)号：WO2014092993A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/071633
申请日：2013-11-25
申请人： SAPPHIRE ENERGY, INC.
发明人： LANGER, Timothy , ARAVANIS, Alex , MATSUMOTO, Shinsyu
IPC分类号： C12N1/02 , C12N1/12
CPC分类号： C12N1/12 , C12N1/02
摘要： The present application includes methods to harvest a non-vascular photosynthetic organism (NVPO) such as microalgae from an aqueous culture comprising brackish, non-brackish, marine, sea or saline water using polymer flocculants. The methods are suitable for harvesting NVPO from aqueous culture with total dissovled solids (TDS) of at least 1500 mg/L. Methods are also provided to harvest a NVPO using flocculation with or without a Dissolved Air Flotation (DAF) process. Methods are further provided to flocculate and harvest a NVPO directly in a pond. The present application further provides NVPO-containing intermediates, compositions, or products produced by the methods provided herein.
摘要翻译：本申请包括使用聚合物絮凝剂从含水培养物中收获非血管光合生物（NVPO）如微藻的方法，所述水性培养物包括微咸，非咸淡，海洋，海水或盐水。该方法适用于从含水培养物中收集至少1500mg / L的完全脱溶固体（TDS）的NVPO。还提供了使用具有或不具有溶解空气浮选（DAF）方法的絮凝来收获NVPO的方法。还提供了在池塘内直接絮凝和收获NVPO的方法。本申请还提供了通过本文提供的方法生产的含有NVPO的中间体，组合物或产品。

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