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1. 发明申请

WO1995017524A3 AUTOMATIC GENOTYPE DETERMINATION 审中-公开
公开(公告)号：WO1995017524A3
公开(公告)日：1995-06-29
申请号：PCT/US1994014836
申请日：1994-12-22
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , LINCOLN, Stephen, E. , KNAPP, Michael, R.
IPC分类号： C12Q01/68

2. 发明申请

WO1995015970A1 METHOD FOR THE IMMOBILIZATION OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译：核酸分子的固定化方法
公开(公告)号：WO1995015970A1
公开(公告)日：1995-06-15
申请号：PCT/US1994014096
申请日：1994-12-06
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , NIKIFOROV, Theo , KNAPP, Michael, R.
IPC分类号： C07H19/00
CPC分类号： C12Q1/6834 , C12N15/1006
摘要： An improved method for affecting the immobilization of a synthetic nucleic acid molecule, such as an oligonucleotide, to a solid surface, especially polystyrene or glass. The method is useful in facilitating polymorphic analyses, in hybridization assays, and in solid-phase DNA sequencing.
摘要翻译：用于影响合成核酸分子如寡核苷酸固定在固体表面，特别是聚苯乙烯或玻璃上的改进方法。该方法可用于促进多态性分析，杂交测定和固相DNA测序。

3. 发明申请

WO1997035033A1 METHOD FOR DETERMINING THE NUCLEOTIDE SEQUENCE OF A POLYNUCLEOTIDE 审中-公开
标题翻译：用于测定多核苷酸核苷酸序列的方法
公开(公告)号：WO1997035033A1
公开(公告)日：1997-09-25
申请号：PCT/US1997003701
申请日：1997-03-18
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , BOYCE-JACINO, Michael, T. , ROGERS, Yu-Hui , GOELET, Philip
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2549/119 , C12Q2565/537 , C12Q2535/101 , C12Q2525/179
摘要： The invention provides a kit and a solid phase sequencing method for determining the sequence of nucleic acid molecules, and particularly the sequence of regions of nucleic acid molecules in which variation from the normal sequence is associated with disease.
摘要翻译：本发明提供了用于确定核酸分子序列的试剂盒和固相测序方法，特别是其中来自正常序列的变异与疾病相关的核酸分子的区域序列。

4. 发明申请

WO9517524A2 AUTOMATIC GENOTYPE DETERMINATION 审中-公开
标题翻译：自动基因组测定
公开(公告)号：WO9517524A2
公开(公告)日：1995-06-29
申请号：PCT/US9414836
申请日：1994-12-22
申请人： MOLECULAR TOOL INC
发明人： LINCOLN STEPHEN E , KNAPP MICHAEL R
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6827 , G06F19/18 , G06F19/24
摘要： A method and device are provided for determining the genotype at selected loci within genetic material obtained from a biological sample. One or more data sets are formed and probability distributions established. These distributions associate hypothetical reaction values with corresponding probabilities for each genotype of interest at the same locus or at different loci. The genotype is then determined based on these measures. The foregoing methods have been employed for automatic genotype determination based on assays using genetic bit analysis. The methods of the invention have been embodied in a device suitable for determining the genotype at selected loci within genetic material obtained from the subject.
摘要翻译：提供了一种方法和装置，用于确定从生物样品获得的遗传物质内选定位点的基因型。形成一个或多个数据集并建立概率分布。这些分布将假设反应值与相同基因座处或不同基因座处的感兴趣基因型的相应概率相关联。然后基于这些措施确定基因型。基于使用遗传位分析的测定，已经使用上述方法用于自动基因型测定。本发明的方法已被体现在适于确定从受试者获得的遗传物质内的选定基因座的基因型的装置中。

5. 发明申请

WO1994016090A1 METHOD FOR GENERATING SINGLE-STRANDED DNA MOLECULES 审中-公开
标题翻译：用于产生单链DNA分子的方法
公开(公告)号：WO1994016090A1
公开(公告)日：1994-07-21
申请号：PCT/US1994000771
申请日：1994-01-18
申请人： MOLECULAR TOOL, INC. , NIKIFOROV, Theo , KNAPP, Michael, R.
发明人： MOLECULAR TOOL, INC.
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12N15/10 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , Y10S435/81 , C12Q2521/301
摘要： A method for generating single-stranded nucleic acid molecules. The molecules contain nuclease resistant modified nucleotides, such that they are resistant to 5' -> 3' exonucleases.
摘要翻译：一种产生单链核酸分子的方法。分子含有核酸酶抗性修饰的核苷酸，使得它们对5'→3'外切核酸酶具有抗性。

6. 发明申请

WO1995021271A1 LIGASE/POLYMERASE-MEDIATED GENETIC BIT ANALYSISTM OF SINGLE NUCLEOTIDE POLYMORPHISMS AND ITS USE IN GENETIC ANALYSIS 审中-公开
标题翻译：单核苷酸多态性的基因/聚合酶介导的遗传分析分析及其在遗传分析中的应用
公开(公告)号：WO1995021271A1
公开(公告)日：1995-08-10
申请号：PCT/US1995001639
申请日：1995-02-07
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , NIKIFOROV, Theo , KARN, Jonathan , GOELET, Philip
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6862 , Y10S435/81 , C12Q2565/537 , C12Q2561/125 , C12Q2565/518 , C12Q2535/125
摘要： A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
摘要翻译：提供了用于确定核酸分子中预选位点处的核苷酸的身份的方法。该方法包括将与预选位点处存在的核苷酸互补的核苷三磷酸引入引物分子的末端，并且随后与第二寡核苷酸连接。通过检测连接到反应的固相的特定标记或通过在溶液中检测来监测反应。

7. 发明申请

WO1995012607A1 SINGLE NUCLEOTIDE POLYMORPHISMS AND THEIR USE IN GENETIC ANALYSIS 审中-公开
标题翻译：单核苷酸多态性及其在遗传分析中的应用
公开(公告)号：WO1995012607A1
公开(公告)日：1995-05-11
申请号：PCT/US1994012632
申请日：1994-11-02
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , GOELET, Philip , KNAPP, Michael, R.
IPC分类号： C07H21/04
CPC分类号： C07H21/00 , C12Q1/6858 , C12Q2565/537 , C12Q2535/125 , C12Q2525/186
摘要： Molecules and methods suitable for identifying polymorphic sites in the genome of a plant or animal. The identification of such sites is useful in determining identity, ancestry, predisposition to genetic disease, the presence or absence of a desired trait, etc.
摘要翻译：适用于鉴定植物或动物基因组中多态性位点的分子和方法。这些位点的鉴定在确定遗传疾病的身份，祖先，倾向，所需特征的存在或不存在等方面是有用的。

8. 发明申请

WO1992015712A1 NUCLEIC ACID TYPING BY POLYMERASE EXTENSION OF OLIGONUCLEOTIDES USING TERMINATOR MIXTURES 审中-公开
标题翻译：使用终端混合物的聚合酶扩增寡核苷酸的核酸类型
公开(公告)号：WO1992015712A1
公开(公告)日：1992-09-17
申请号：PCT/US1992001905
申请日：1992-03-04
申请人： MOLECULAR TOOL, INC.
发明人： MOLECULAR TOOL, INC. , GOELET, Philip , KNAPP, Michael, R. , ANDERSON, Stephen
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2535/125 , C12Q2535/101 , C12Q2563/107 , C12Q2537/143 , C12Q2525/121
摘要： This invention concerns a reagent composition comprising at least two different terminators of a nucleic acid template-dependent, primer extension reaction. This invention also concerns a method for determining the identity of a nucleotide base at a specific position in a nucleic acid of interest. This invention further concerns a method for determining the presence or absence of a particular nucleotide sequence in a sample of nucleic acids. This invention further concerns a method for identifying different alleles in a sample containing nucleic acids. This invention further concerns a method for determining the genotype of an organism at one or more particular genetic loci.
摘要翻译：本发明涉及包含核酸模板依赖性引物延伸反应的至少两种不同终止子的试剂组合物。本发明还涉及用于确定感兴趣的核酸中特定位置的核苷酸碱基的身份的方法。本发明还涉及用于确定核酸样品中特定核苷酸序列的存在或不存在的方法。本发明还涉及用于鉴定含有核酸的样品中的不同等位基因的方法。本发明还涉及用于确定一个或多个特定遗传基因座上的生物体的基因型的方法。

9. 发明专利

AU682741B2 Method for the immobilization of nucleic acid molecules 未知
公开(公告)号：AU682741B2
公开(公告)日：1997-10-16
申请号：AU1303295
申请日：1994-12-06
申请人： MOLECULAR TOOL INC
发明人： NIKIFOROV THEO , KNAPP MICHAEL R
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/00 , C12N11/08 , C07H21/04
摘要： Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an -OH, -C=O or -COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.

10. 发明专利

AU2320597A Method for determining the nucleotide sequence of a polynucleotide 未知
公开(公告)号：AU2320597A
公开(公告)日：1997-10-10
申请号：AU2320597
申请日：1997-03-18
申请人： MOLECULAR TOOL INC
发明人： BOYCE-JACINO MICHAEL T , ROGERS YU-HUI , GOELET PHILIP
IPC分类号： C12Q1/68 , C12P19/34
摘要： The present invention provides a method for determining the nucleotide sequence of a nucleic acid molecule using primer oligonucleotides arrayed on a solid support and doing primer extension. By providing sequence information from both oligonucleotide primers in which the 3' terminal nucleotide of the oligonucleotide primer is hybridized to the nucleic acid molecule and oligonucleotide primers in which the 3' terminal nucleotide is not hybridized to the nucleic acid molecule the invention is advantageous in that less primers are needed to fully sequence a nucleic acid molecule. Where the 3' terminal nucleotide of an oligonucleotide in the array does not hybridize to the nucleic acid molecule, the present invention provides for either not permitting oligonucleotides hybridized to nucleic acid molecules to be extended by polymerase-mediated incorporation of a single chain terminator nucleotide residue onto the 3' terminus of said hybridized oligonucleotide, or permitting the removal of any non-hybridized nucleotide residues from the 3' terminus of said hybridized oligonucloetides, so as to form a truncated primer oligonucleotide whose 3' terminus is hybridized to said nucleic acid molecule. The invention also provides kits that can be used to implement the methods of the invention.

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