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1. 发明申请

WO2007043114A9 SOY SAUCE CONTAINING 5'-NUCLEOTIDES AND METHOD OF PRODUCING THE SAME 审中-公开
标题翻译：含有5'-核苷酸的大豆及其生产方法
公开(公告)号：WO2007043114A9
公开(公告)日：2007-05-31
申请号：PCT/JP2005018165
申请日：2005-09-30
申请人： KIKKOMAN CORP , HANADA YOICHI , TSUJI RYOHEI
发明人： HANADA YOICHI , TSUJI RYOHEI
IPC分类号： A23L1/238
CPC分类号： A23L33/13 , A23L27/50
摘要： A method of producing a soy sauce containing 5'-nucleotides characterized in that, in a method of producing soy sauce, 5'-nucleotides are formed and accumulated by adding malt, germinating rice or a processed product thereof to say sauce mash while inactivating microorganism-origin phosphatases; and a soy sauce containing 5'-nucleotides which is obtained by this method.
摘要翻译：一种含有5'-核苷酸的酱油的制造方法，其特征在于，在制造酱油的方法中，通过添加麦芽，发芽稻或其加工品形成并积累5'-核苷酸，以说灭活微生物 - 原料磷酸酶; 和通过该方法得到的含有5'-核苷酸的酱油。

2. 发明申请

WO0031245A8 CREATINE AMIDINOHYDROLASE AND PROCESS FOR PRODUCING THE SAME 审中-公开
标题翻译： CRETINE AMININOHYDROLASE AND PROCESS FOR PRODUCTION THE SAME
公开(公告)号：WO0031245A8
公开(公告)日：2001-11-15
申请号：PCT/JP9906583
申请日：1999-11-25
申请人： KIKKOMAN CORP , FURUKAWA KEISUKE , ICHIKAWA TOSHIO
发明人： FURUKAWA KEISUKE , ICHIKAWA TOSHIO
IPC分类号： C12N15/09 , C12N1/21 , C12N9/78 , C12N9/80 , C12N15/00 , C12R1/05 , C12R1/19
CPC分类号： C12N9/78 , C12Y305/03003
摘要： A creatine amidinohydrolase having the following physicochemical properties: (a) hydrolyzing 1 mol of creatine to give 1 mol of sarcocine and 1 mol of urea; (b) having a substrate specificity for creatine; (c) having an optimum pH value of 6 to 7, in particular about 6.5; (d) being stable within a pH range of 4 to 11.0; (e) having an optimum working temperature range of 50 to 55 DEG C; and (f) having a molecular weight of about 92,000 Da (measured by gel filtration); and a process for producing this creatine amidinohydrolase which comprises culturing a microorganism capable of producing the creatine amidinohydrolase and collecting the creatine amidinohydrolase from the culture medium. Because of having the optimum pH value in the weakly acidic region, the creatine amidinohydrolase has a characteristic of being scarcely affected by bilirubin in assaying creatinine.
摘要翻译：具有以下物理化学性质的肌酸脒基水解酶：（a）水解1摩尔肌酸，得到1摩尔肌醇和1摩尔尿素; （b）对肌酸具有底物特异性; （c）最佳pH值为6至7，特别是约6.5; （d）在4至11.0的pH范围内稳定; （e）最佳工作温度范围为50至55℃; 和（f）分子量约92,000Da（通过凝胶过滤测量）; 以及生产该肌酸脒基水解酶的方法，其包括培养能够产生肌酸脒基水解酶的微生物并从培养基中收集肌酸脒基水解酶。由于在弱酸性区域具有最佳的pH值，所以肌酸脒基水解酶具有在测定肌酐中几乎不受胆红素影响的特征。

3. 发明公开

KR20200142012A 신규 메디에이터 审中-公开
公开(公告)号：KR20200142012A
公开(公告)日：2020-12-21
申请号：KR20207029617
申请日：2019-02-26
申请人： KIKKOMAN CORP
发明人： MASAKARI YOSUKE , TOTSUKA NAOYA , SHINOHARA YASUTOMO
IPC分类号： H01M8/16 , C07C211/55 , G01N27/327 , G01N27/416 , H01M4/90
摘要： 신규의메디에이터를제공하는것. 본발명은신규의메디에이터및 이를포함하는전극개변제, 전자전달촉진제, 이들을포함하는전극, 전지, 조성물, 효소센서및 이들을사용하는방법에관한것이다.

4. 发明公开

EP3031914A4 MODIFIED AMADORIASE AND METHOD FOR PRODUCING SAME, AGENT FOR IMPROVING SURFACTANT-RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING SAME 有权
标题翻译：改性阿马AND METHOD FOR PRODUCING物的活性，以改善表面活性剂抗性阿马和组合物因此测量HBA1
公开(公告)号：EP3031914A4
公开(公告)日：2017-01-11
申请号：EP14834684
申请日：2014-08-08
申请人： KIKKOMAN CORP
发明人： MASAKARI YOSUKE , ICHIYANAGI ATSUSHI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/06 , C12Q1/26 , G01N33/72
CPC分类号： C12Q1/26 , C12N9/0032 , C12Y105/03 , G01N33/723 , G01N2333/805 , G01N2333/90672
摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.
摘要翻译：提供了通过该糖化血红蛋白甚至可以在表面活性剂比常规情况下更强的存在下测得的组合物。如此提供的是其保持阿马道里的残余活性或降低的降低残留活性的缓冲液和/或稳定剂。本发明提供了在测量糖化血红蛋白在阿马含有具有一个或多个氨基酸残基取代在在选自262位选择的氨基酸（S）对应于位置（S），位置257中使用的组合物，位置249，位置253，位置337，位置340，位置232，位置129，位置132，位置133，位置44，位置256，位置231和从该属锥毛壳衍生和由SEQ ID NO的阿马道里酶的位置81： 1或3，并且具有即使在表面活性剂存在的残余活性。本发明因此提供了在测量糖化血红蛋白中使用的组合物和试剂盒，包括一个特定的稳定剂和/或缓冲剂。本发明可以提供酶和测量糖化血红蛋白，具有优异的储存稳定性，即使它们被暴露到表面活性剂中使用的组合物。

5. 发明公开

EP2915430A4 POWDERY SEASONING AND METHOD FOR MANUFACTURING SAME 有权
标题翻译：其制造粉状SPICE和方法
公开(公告)号：EP2915430A4
公开(公告)日：2016-06-01
申请号：EP13851857
申请日：2013-10-29
申请人： KIKKOMAN CORP
发明人： NAKAMURA SATORU , TAKAHAGI YASUSHI
IPC分类号： A23L27/00 , A23L27/21 , A23L27/50
CPC分类号： A23L1/238 , A23L27/00 , A23L27/21 , A23L27/50 , A23V2002/00 , A23V2200/15 , A23V2300/10 , A23V2300/12
摘要： The present invention relates to a powdery seasoning produced by drying and powdering a seasoning liquid obtained by enzymatically decomposing a protein-containing raw material derived from at least one of wheat and a soybean in the presence of salt, which has an inorganic material concentration of 50000 ppm or less, and a method for producing the same.

6. 发明公开

EP2719761A4 FLAVIN-BINDING GLUCOSE DEHYDROGENASE, METHOD FOR MANUFACTURING FLAVIN-BINDING GLUCOSE DEHYDROGENASE, AND GLUCOSE MEASUREMENT METHOD USING SAME 有权
标题翻译：黄素结合型葡萄糖，法制造约束力的黄素葡萄糖和血糖测量的方式，该方式
公开(公告)号：EP2719761A4
公开(公告)日：2015-01-21
申请号：EP12797050
申请日：2012-06-06
申请人： KIKKOMAN CORP
发明人： TAJIMA RYOKO , HIROKAWA KOZO , YOSHIHARA ERIKO , TANABE YASUKO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12Q1/00 , C12Q1/32 , C12Q1/54
CPC分类号： C12Q1/54 , C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Y101/9901 , G01N2333/904
摘要： Provided is a flavin-binding glucose dehydrogenase (FAD-GDH), which in addition to having high substrate specificity and adequate heat stability, is suitable for efficient production preferably using E. coli, yeast or molds and the like as host cells. This FAD-GDH has amino acid substitutions at positions equivalent to one or more locations of the group consisting of position 213, position 368 and position 526 in the amino acid sequence described in SEQ ID NO: 8. The FAD-GDH is acquired from a culture by inserting a gene encoding this FAD-GDH into host cells such as E. coli. A preferable example of the FAD-GDH of the present invention is FAD-GDH, in which a signal peptide region present in an N-terminal region has been deleted from the amino acid sequence of Mucor-derived FAD-GDH, and which has the aforementioned amino acid substitutions. FAD-GDH can be efficiently produced that has high substrate specificity and superior heat stability and can be preferably used in clinical diagnosis and the like.

7. 发明公开

EP2647703A4 E. COLI TRANSFORMANT, PRODUCTION METHOD FOR FLAVIN-BOUND GLUCOSE DEHYDROGENASE USING SAME, AND MUTANT FLAVIN-BOUND GLUCOSE DEHYDROGENASE 审中-公开
标题翻译：转基因大肠杆菌，方法FLAVINGEBUNDENE葡萄糖脱氢酶订单和突变FLAVINGEBUNDENE葡萄糖脱氢酶
公开(公告)号：EP2647703A4
公开(公告)日：2014-05-14
申请号：EP11845150
申请日：2011-11-30
申请人： KIKKOMAN CORP
发明人： TAJIMA RYOKO , ICHIYANAGI ATSUSHI , YOSHIHARA ERIKO , HIROKAWA KOZO
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12P21/02
CPC分类号： C12N9/0006

8. 发明公开

EP2420561A4 ASPERGILLUS SP. HAVING LARGE-SCALE GENOME DUPLICATION 有权
标题翻译： ASPERGILLUS SP。 MIT MASSENGENOMDUPLISIERUNG
公开(公告)号：EP2420561A4
公开(公告)日：2014-01-22
申请号：EP10764549
申请日：2010-04-16
申请人： KIKKOMAN CORP
发明人： SATO ATSUSHI , HANYA YOSHIKI
IPC分类号： A23L27/24 , A23L27/21 , A23L27/50 , C12N9/30 , C12N9/62 , C12R1/66 , C12R1/69
CPC分类号： A23L1/238 , A23L2/84 , A23L27/50 , C12N9/242 , C12N9/58 , C12N9/62 , C12N15/52 , C12R1/66 , C12R1/69 , C12Y302/01001
摘要： The purpose of the present invention is to breed a koji mold, which has improved capability of producing a variety of industrially applicable enzymes, without using the genetic recombination technology. By mutagenizing a strain belonging to the genus Aspergillus, a strain having a duplication- of a large-scale genome region of 900 kb or more and is able to produce a variety of enzymes required in manufacturing soy sauce, for example, protease, etc. was obtained. This strain enables efficient production of various foods such as soy sauce.
摘要翻译：本发明的目的是在不使用遗传重组技术的情况下培育具有提高的各种工业应用酶的生产能力的曲霉菌。通过诱变属于曲霉属的菌株，具有900kb或更高的大规模基因组区域的重复并且能够产生在生产酱油中所需的各种酶例如蛋白酶等的菌株被获得。这种菌株可以高效生产各种食物，如酱油。

9. 发明公开

EP2269473A4 REDUCED-SODIUM MOROMI SOY SAUCE 审中-公开
标题翻译： NATRIUMREDUZIERTE醪SOJASOSSE
公开(公告)号：EP2269473A4
公开(公告)日：2013-10-09
申请号：EP08873536
申请日：2008-12-29
申请人： KIKKOMAN CORP
发明人： KOBAYASHI EMI
IPC分类号： A23L27/50
CPC分类号： A23L27/50

10. 发明公开

EP2499923A4 LIQUID SEASONING HAVING IMPROVED FLAVOR 有权
标题翻译：具有改进的味液SEASONINGS
公开(公告)号：EP2499923A4
公开(公告)日：2013-04-24
申请号：EP10828404
申请日：2010-11-09
申请人： KIKKOMAN CORP
发明人： TSUCHIYA KATSUNORI , UCHIDA RIICHIRO , TODA YASUKA
IPC分类号： A23L27/50 , A23L27/40
CPC分类号： A23L27/50 , A23L27/40

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