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1. 发明专利

JP2014132899A Device for moving cultured cells and method for using the same 有权
标题翻译：用于移动培养的细胞的装置和使用它们的方法
公开(公告)号：JP2014132899A
公开(公告)日：2014-07-24
申请号：JP2014008851
申请日：2014-01-21
申请人： Cellseed Inc , 株式会社セルシード
发明人： WATANABE HIROYA , ASHIBA KEISUKE , MIZUTANI MANABU
IPC分类号： C12M3/00 , A61L27/00 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide a device for moving cultured cells which can efficiently exfoliate the cultured cells within any range on a cell culture base material and adhere the exfoliated cells again easily.SOLUTION: The device for moving cultured cells has a convex cell adhesion surface for exfoliating the cultured cells on the cell culture base material surface coated with a temperature-responsive polymer by means of temperature change, then adhering the exfoliated cells to another place again, the cell adhesion surface being an elastic body.
摘要翻译：要解决的问题：提供一种用于移动培养细胞的装置，其能够在细胞培养基材上的任何范围内有效地剥离培养的细胞，并且容易地粘附剥离的细胞。解决方案：用于移动培养细胞的装置具有凸细胞粘附表面，用于通过温度变化将经过温度响应的聚合物涂覆的细胞培养基材表面上的培养细胞剥离，然后再次将剥离的细胞粘附到另一个位置，细胞粘附表面是弹性体。

2. 发明专利

JP2013143955A Supply system for closed culture medium for culturing small cell 审中-公开
标题翻译：用于培养小细胞的封闭文化培养基的供应系统
公开(公告)号：JP2013143955A
公开(公告)日：2013-07-25
申请号：JP2013057193
申请日：2013-03-19
申请人： Cellseed Inc , 株式会社セルシード
发明人： MIZUTANI MANABU
IPC分类号： C12N5/10 , C12M3/00
摘要： PROBLEM TO BE SOLVED: To provide a method for culturing cells using a supply system for closed culture medium for culturing small cells characterized by culturing the cells during moving or carrying the supply system for the closed culture medium for culturing small cells.SOLUTION: Cells are cultured during moving or carrying using the closed small cell culture medium supply system in which a closed cell culture vessel, having a gas-permeable membrane for exchanging a gas in a culture medium and further having a passage for circulating the culture medium, is mounted, the supply of a fresh culture medium is controlled by a liquid feed pump through a piping connected to the culture medium gateway of the vessel, and cells are not contaminated in the vessel.
摘要翻译：要解决的问题：提供一种使用用于培养小细胞的封闭培养基的供给系统培养细胞的方法，其特征在于在移动或携带用于培养小细胞的封闭培养基的供给系统中培养细胞。解决方案：细胞是在移动或携带时使用封闭的小细胞培养基供应系统进行培养，其中安装具有用于在培养基中更换气体并且还具有用于循环培养基的通道的气体透过膜的闭孔培养容器，新鲜培养基的供应通过液体供给泵通过连接到容器的培养基质网关的管道来控制，并且细胞在容器中不被污染。

3. 发明专利

JP2013005789A Expandable temperature-responsive base material, method for production thereof, and method for use thereof 有权
标题翻译：可膨胀的温度响应基材，其生产方法及其使用方法
公开(公告)号：JP2013005789A
公开(公告)日：2013-01-10
申请号：JP2011152308
申请日：2011-06-22
申请人： Cellseed Inc , 株式会社セルシード , Tokyo Women's Medical College , 学校法人東京女子医科大学
发明人： AKIYAMA YOSHIKATSU , OKANO MITSUO
IPC分类号： C12M3/00 , C12M1/00 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide a base material capable of forming surface states of multiple conditions from a surface of one temperature-responsive base material.SOLUTION: The temparature-responsive base material is used in which the surface of the base material is covered with a temperature-responsive polymer in which hydrating force changes in the temperature range of 0 to 80°C and affinity with a living body material can be changed by extending and/or contracting the surface.
摘要翻译：要解决的问题：提供能够从一个温度敏感性基材的表面形成多种条件的表面状态的基材。解决方案：使用温度敏感性基材，其中基材的表面被温度响应性聚合物覆盖，其中水合力在0至80℃的温度范围内变化并且与活体亲和力可以通过延伸和/或收缩表面来改变材料。版权所有（C）2013，JPO＆INPIT

4. 发明专利

JP2010094114A Implement for transferring cultured cell, and method for utilizing the same 审中-公开
标题翻译：用于传送培养的细胞的实现，以及使用它们的方法
公开(公告)号：JP2010094114A
公开(公告)日：2010-04-30
申请号：JP2008288219
申请日：2008-10-14
申请人： Cellseed Inc , 株式会社セルシード
发明人： MIZUTANI MANABU , KIKUCHI TETSUTARO
IPC分类号： C12M3/00 , C12N5/07
CPC分类号： C12M33/00 , C12M25/02 , C12M25/14
摘要： PROBLEM TO BE SOLVED: To efficiently separate a cultured cell within any range on a cell-culturing substrate and then attach the separated the cultured cell again.
SOLUTION: An implement for transferring a cultured cell is provided, which has such a cell-binding part with a convex form under non-loaded state in such a way as to begin to contact from its convex chip, and finally, enables a whole surface of a cell-culturing substrate to be contacted.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：为了有效地将培养细胞分离在细胞培养基底上的任何范围内，然后再次将培养细胞分离。提供了一种用于转移培养细胞的装置，其在非负载状态下具有这样的具有凸形状的细胞结合部分，以便从其凸起的芯片开始接触，最后，使能待接触的细胞培养基底的整个表面。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2009082123A Evaluation method of cell culture substrate 有权
标题翻译：细胞培养基质的评价方法
公开(公告)号：JP2009082123A
公开(公告)日：2009-04-23
申请号：JP2007282412
申请日：2007-10-02
申请人： Cellseed Inc , 株式会社セルシード
发明人： ASHIBA KEISUKE
IPC分类号： C12Q1/02 , C12M3/00 , C12N5/07 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide an evaluation method of a temperature responsive cell culture substrate covered with a temperature responsive polymer whose hydration force changes in a temperature range of 0-80°C. SOLUTION: The cell is exfoliated and evaluated within 24 hr after seeding the established cell line on the surface of a temperature responsive cell culture substrate without using a protein hydrolysis enzyme but only by thermal treatment. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用温度响应性聚合物覆盖的温度敏感性细胞培养基质的评价方法，其温度在0-80℃的温度范围内变化。解决方案：在不使用蛋白质水解酶但仅通过热处理的情况下，将建立的细胞系接种在温度敏感细胞培养基质的表面上的24小时内，细胞进行剥离和评价。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2008241679A Separation method of physiologically active polymeric substance 有权
标题翻译：生理活性聚合物质的分离方法
公开(公告)号：JP2008241679A
公开(公告)日：2008-10-09
申请号：JP2007114834
申请日：2007-03-27
申请人： Cellseed Inc , 株式会社セルシード
发明人： SUGA HIROSHI
IPC分类号： G01N30/88 , B01D15/08 , G01N30/26
摘要： PROBLEM TO BE SOLVED: To provide a separation method of a physiologically active polymeric substance. SOLUTION: An operation is performed, wherein a stationary phase surface is coated with a polymer having the charge whose hydration force is changed in the range of 0-80°C by using neutral buffer solution having a low salt concentration as a moving phase, and the polymeric substance is held by shrinking a polymer chain on the stationary phase surface and desorbed by swelling the polymer chain by using a filling material capable of changing an effective charge density on the stationary phase surface by shrinking and swelling of the polymer chain caused by a temperature change. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供生理活性聚合物质的分离方法。
解决方案：执行一个操作，其中通过使用具有低盐浓度的中性缓冲溶液作为移动的静电相表面涂覆有具有水合力在0-80℃范围内变化的电荷的聚合物相，并且聚合物通过在固定相表面上收缩聚合物链而被保持，并且通过使用能够通过聚合物链的收缩和溶胀改变固定相表面上的有效电荷密度的填充材料而使聚合物链膨胀来解吸造成温度变化。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2008231083A Maturation-induced dendritic cell for transplant, its preparation method and its usage 有权
标题翻译：成熟诱导的转运细胞，其制备方法及其用途
公开(公告)号：JP2008231083A
公开(公告)日：2008-10-02
申请号：JP2007107583
申请日：2007-03-19
申请人： Cellseed Inc , Keiichi Konya , 株式会社セルシード , 桂一紺谷
发明人： KONYA KEIICHI , MASUDA AKIRA
IPC分类号： A61K35/12 , A61P31/12 , A61P35/00 , A61P37/02 , A61P37/06 , A61P37/08 , C12N5/07 , C12N5/0784 , C12N5/0786 , C12N5/09
摘要： PROBLEM TO BE SOLVED: To provide maturation-induced dendritic cells for transplant, which are subjected to maturation induction ex vivo, are suspended in a medium and have little damage on cell-surface antigens. SOLUTION: Immature dendritic cells are seeded on a surface of a substrate which is coated with 1.3-2.2 μg/cm 2 temperature-responsive polymer whose upper or lower critical solution temperature in water is 0-80°C. After their maturation is induced using picibanil, the temperature of the culture liquid is regulated to a temperature not lower than the upper-limit critical solubilization temperature or not higher than the lower-limit critical solubilization temperature, and the matured dendritic cells are peeled off without subjecting them to any enzymatic treatment. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：为了提供成熟诱导的用于移植成熟的树突细胞，其离体成熟诱导悬浮在培养基中并且对细胞表面抗原几乎没有损伤。解决方案：将未成熟的树突状细胞接种在底物的表面上，该表面涂有1.3-2.2微克/厘米2 / SP>温度响应聚合物，其水中的临界溶解温度或其临界溶解温度为0- 80℃。使用苦参碱诱导成熟后，将培养液的温度调节至不低于上限临界溶解温度或不高于下限临界溶解温度的温度，并将成熟树突状细胞剥离而无对其进行任何酶处理。版权所有（C）2009，JPO＆INPIT

8. 发明专利

JP2005292106A Manufacturing method of temperature responsive liquid chromatography carrier 有权
标题翻译：温度响应液体色谱载体的制造方法
公开(公告)号：JP2005292106A
公开(公告)日：2005-10-20
申请号：JP2004134822
申请日：2004-03-31
申请人： Cellseed Inc , Masaaki Kakimoto , 雅明柿本 , 株式会社セルシード
发明人： SUGA HIROSHI , SAKAI HIDEAKI , KAKIMOTO MASAAKI
IPC分类号： B01J20/285 , B01J20/283 , G01N30/54 , G01N30/88 , G01N30/48
摘要： PROBLEM TO BE SOLVED: To provide a manufacturing method of a temperature responsive liquid chromatography carrier.
SOLUTION: The fixing amount of a polymer to the surface of a chromatography carrier is controlled by grafting another polymer to a dendritic polymer having a siloxane skeleton.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种温度敏感性液相色谱载体的制造方法。解决方案：通过将另一种聚合物接枝到具有硅氧烷骨架的树枝状聚合物来控制聚合物对色谱载体表面的固定量。版权所有（C）2006，JPO＆NCIPI

9. 发明专利

JP2014155494A Cultured cell sheet, production method thereof, and application method thereof 有权
标题翻译：培养细胞片，其生产方法及其应用方法
公开(公告)号：JP2014155494A
公开(公告)日：2014-08-28
申请号：JP2014078705
申请日：2014-04-07
申请人： Cellseed Inc , 株式会社セルシード , Tokai Univ , 学校法人東海大学
发明人： SATO MASATO , SAKAI HIDEAKI
IPC分类号： C12N5/077 , A61L27/00 , C12N5/0775
CPC分类号： C12N5/0655 , A61K35/12 , A61L27/3817 , A61L2430/06 , C12N2539/10
摘要： PROBLEM TO BE SOLVED: To provide a cultured cell sheet allowing a character to be expressed as a chondroid tissue.SOLUTION: The problem can be solved by culturing cells in high density on a cell culture support in which the surface of a substrate is coated with a temperature responsive polymer having an upper or lower critical dissolution temperature in water ranging from 0 to 80°C and producing the cultured cell sheet by (1) setting a culture medium temperature to not lower than the upper critical dissolution temperature or not higher than the lower critical dissolution temperature, (2) bringing the resulting cultured cell sheet into contact with a carrier, and (3) detaching the sheet as such with the carrier.
摘要翻译：要解决的问题：提供允许角色被表达为软骨组织的培养细胞片。解决方案：可以通过在其上涂覆有基材的表面的细胞培养载体上高密度培养细胞来解决问题一种温度响应性聚合物，其在0-80℃的水中具有上限或下限临界溶解温度，并通过（1）将培养基温度设定为不低于上临界溶解温度或不高于降低临界溶解温度，（2）使得到的培养细胞片与载体接触，和（3）用载体将片材分离。

10. 发明专利

JP2013106924A Temperature-responsive chondrocyte-containing fluid formulation for cartilaginous tissue regeneration, production method, and utilization method of the same 有权
标题翻译：用于卡培他酮组织再生的温度有效的含有液体的液体制剂，其生产方法及其利用方法
公开(公告)号：JP2013106924A
公开(公告)日：2013-06-06
申请号：JP2011268341
申请日：2011-11-19
申请人： Cellseed Inc , 株式会社セルシード , Kazuaki Muramatsu , 和明村松
发明人： MURAMATSU KAZUAKI
IPC分类号： A61L27/00 , A61K35/32 , A61K38/27 , A61K47/32 , A61K47/36 , A61P19/02 , C12N5/077
摘要： PROBLEM TO BE SOLVED: To provide a cartilaginous tissue regeneration medial formulation capable of increasing phenotypic expressions of chondrocytes thereby suppressing inflammation of a recipient site.SOLUTION: In a temperature-responsive chondrocyte-containing fluid formulation, hyaluronic acid grafted to a polymer whose hydration force changes in the temperature range of 0-80°C is dissolved in saline, and 1×10pieces to 1×10pieces of cells are dispersed per ml of the formulation.

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