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    • 3. 发明申请
    • METHOD FOR DISTINGUISHING AND SORTING OF CELLS AND DEVICE THEREFOR
    • 用于细胞分离和分离的方法及其装置
    • US20110177544A1
    • 2011-07-21
    • US13001128
    • 2009-06-19
    • Toru TakahashiKen TsukiiJie Xu
    • Toru TakahashiKen TsukiiJie Xu
    • C12Q1/02C12M1/34
    • G01N15/147G01N2015/1438G01N2015/1493G01N2015/1497
    • A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.
    • 一种用于区分和分选细胞的方法,其特征在于包括使用反映细胞形态学特征的透射光数据(例如尺寸和形状)来选择性地与侧面区分和分选细胞块中的特定细胞块或细胞的一部分 反映细胞内部结构特征的散射光数据。 如上所述的特定细胞团中的细胞的部分在细胞周期中处于G1阶段或M阶段的一部分。 在G1阶段的细胞的一部分在细胞的分析色散图中被称为左下底线,其中横坐标表示透射光数据,而M阶段的一部分细胞被称为右 细胞的分析色散图中的底线,其中横坐标表示透射的光数据。
    • 5. 发明申请
    • Methods of Separating, Identifying and Dispensing Specimen and Device Therefor, and Analyzing Device Method
    • 分离,鉴定和分配样品及其装置的方法和分析装置方法
    • US20080257072A1
    • 2008-10-23
    • US11587210
    • 2005-04-25
    • Toru TakahashiKen TsukiiJie Xu
    • Toru TakahashiKen TsukiiJie Xu
    • B01L3/02A61L2/20G01N23/00G01P3/36
    • G01N15/1459B01F5/0057B01F11/0074B07C5/00G01N1/286G01N15/1427G01N15/1436G01N2015/1075G01N2035/1062
    • [PROBLEMS] A (a) (b) separation unit can confirms a separation state, a detection unit enhances a lighting efficiency and a light reception sensitivity, and a dispensing unit ensures the normal state of a specimen. [MEANS FOR SOLVING PROBLEMS] A specimen separation device characterized by comprising a container for storing a specimen, a nozzle for sucking and ejecting the specimen from the container, a nozzle operating means for moving the nozzle vertically and laterally, and a nozzle controlling means for controlling the suction force and ejection force of the nozzle. A specimen identification device comprising a nozzle for sucking a specimen from a container storing the specimen, a flow path for allowing the specimen to flow therethrough, and a measuring unit having a monitoring light lighting unit and a light receiving unit for observing (monitoring) the specimen that are installed in a flow path, characterized in that the above nozzle, the flow path and the measuring unit are formed in an integrated structure.
    • (问题)A(a)(b)分离单元可以确认分离状态,检测单元提高照明效率和光接收灵敏度,并且分配单元确保样本的正常状态。 用于解决问题的手段一种试样分离装置,其特征在于,包括容纳容器的容器,用于从容器吸取和喷射试样的喷嘴,用于使喷嘴垂直和侧向移动的喷嘴操作装置,以及用于 控制喷嘴的吸力和喷射力。 一种样本识别装置,包括:用于从容纳所述样本的容器吸取试样的喷嘴,用于使所述试样流过的流路;以及具有监视用光照射单元的测量单元和观察(监视) 试样,其安装在流路中,其特征在于,上述喷嘴,流路和测量单元形成为一体的结构。
    • 6. 发明授权
    • Method for distinguishing and sorting of cells and device therefor
    • 用于区分和分类细胞的方法及其装置
    • US08942458B2
    • 2015-01-27
    • US13001128
    • 2009-06-19
    • Toru TakahashiKen TsukiiJie Xu
    • Toru TakahashiKen TsukiiJie Xu
    • G06K9/00G01N15/14
    • G01N15/147G01N2015/1438G01N2015/1493G01N2015/1497
    • A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.
    • 一种用于区分和分选细胞的方法,其特征在于包括使用反映细胞形态学特征的透射光数据(例如尺寸和形状)来选择性地与侧面区分和分选细胞块中的特定细胞块或细胞的一部分 反映细胞内部结构特征的散射光数据。 如上所述的特定细胞团中的细胞的部分在细胞周期中处于G1阶段或M阶段的一部分。 在G1阶段的细胞的一部分在细胞的分析色散图中被称为左下底线,其中横坐标表示透射光数据,而M阶段的一部分细胞被称为右 细胞的分析色散图中的底线,其中横坐标表示透射的光数据。
    • 7. 发明授权
    • Optical measuring apparatus and specimen discriminating and dispensing apparatus
    • 光学测量装置和试样识别和分配装置
    • US09541489B2
    • 2017-01-10
    • US13176781
    • 2011-07-06
    • Ken TsukiiToru TakahashiJie Xu
    • Ken TsukiiToru TakahashiJie Xu
    • G01F7/00G01N15/14G01F1/708G01F15/07
    • G01N15/14G01F1/7086G01F15/07G01N2015/149
    • The invention provides an optical measuring apparatus having a plurality of measuring sections each having a light irradiating section for irradiating light to specimens and a light receiving section for receiving optical data acquired by irradiating the light to the specimens and a flow rate calculating section for calculating flow rate values of the specimens based on a difference of measured times of the optical data measured by the plurality of measuring sections with respect to the specimens and a distance between the plurality of measuring sections. The optical measuring apparatus measures the optical data of the specimens by irradiating light to the specimens, i.e., the objects to be measured, dispersed within a sample fluid flowing through a flow passage. The optical measuring apparatus further includes a flow rate graph generating section for generating flow rate graph data in which the values of flow rate of the specimens calculated by the flow rate calculating section are arrayed in order of calculation in time-series manner and for outputting the generated flow rate graph data to a displaying section.
    • 本发明提供一种光学测量装置,具有多个测量部分,每个测量部分具有用于向样本照射光的光照射部分和用于接收通过向样品照射光而获得的光学数据的光接收部分和用于计算流量的流量计算部分 基于由多个测量部分测量的光学数据相对于样本的测量次数与多个测量部分之间的距离的差异,样本的速率值。 光学测量装置通过将光照射到流过流动通道的样品流体中的样品(即被测量物体)上来测量样品的光学数据。 光学测量装置还包括:流量图生成部,其生成流量图数据,其中由流量计算部计算出的样本的流量值按时间顺序排列的顺序排列,并输出 生成流量图数据到显示部分。
    • 9. 发明授权
    • Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method
    • 分离,识别和分配样品及其装置的方法,以及分析装置方法
    • US08264674B2
    • 2012-09-11
    • US11587210
    • 2005-04-25
    • Toru TakahashiKen TsukiiJie Xu
    • Toru TakahashiKen TsukiiJie Xu
    • G01N23/00
    • G01N15/1459B01F5/0057B01F11/0074B07C5/00G01N1/286G01N15/1427G01N15/1436G01N2015/1075G01N2035/1062
    • [PROBLEMS] A separation unit can confirms a separation state, a detection unit enhances a lighting efficiency and a light reception sensitivity, and a dispensing unit ensures the normal state of a specimen. [MEANS FOR SOLVING PROBLEMS] A specimen separation device characterized by comprising a container for storing a specimen, a nozzle for sucking and ejecting the specimen from the container, a nozzle operating means for moving the nozzle vertically and laterally, and a nozzle controlling means for controlling the suction force and ejection force of the nozzle. A specimen identification device comprising a nozzle for sucking a specimen from a container storing the specimen, a flow path for allowing the specimen to flow therethrough, and a measuring unit having a monitoring light lighting unit and a light receiving unit for observing (monitoring) the specimen that are installed in a flow path, characterized in that the above nozzle, the flow path and the measuring unit are formed in an integrated structure.
    • [问题]分离单元可以确认分离状态,检测单元提高照明效率和光接收灵敏度,并且分配单元确保样本的正常状态。 用于解决问题的手段一种试样分离装置,其特征在于,包括容纳容器的容器,用于从容器吸取和喷射试样的喷嘴,用于使喷嘴垂直和侧向移动的喷嘴操作装置,以及用于 控制喷嘴的吸力和喷射力。 一种样本识别装置,包括:用于从容纳所述样本的容器吸取试样的喷嘴,用于使所述试样流过的流路;以及具有监视用光照射单元的测量单元和观察(监视) 试样,其安装在流路中,其特征在于,上述喷嘴,流路和测量单元形成为一体的结构。
    • 10. 发明申请
    • OPTICAL INFORMATION ANALYZER AND OPTICAL INFORMATION ANALYSIS METHOD
    • 光信息分析仪和光信息分析方法
    • US20120154804A1
    • 2012-06-21
    • US13337848
    • 2011-12-27
    • Ken TSUKIIKenichi KimuraToru TakahashiJie Xu
    • Ken TSUKIIKenichi KimuraToru TakahashiJie Xu
    • G01N21/49
    • G01N15/1434G01N2015/1452
    • An optical information analyzer (10) includes a light emitting unit (30) that emits light (excitation light) (L0) to a sample (S), a transmission light receiving unit (50) that receives transmission light (L1), which is the excitation light passing through the sample (S), and detects the received transmission light as a transmission light signal (SG1), scattered light/fluorescence receiving units (60) and (70) that are provided at a plurality of positions, receive side scattered light/fluorescence components (L2) and (L3) from the sample (S), and detect the received side scattered light/fluorescence components as scattered light/fluorescence signals (SG2) and (SG3), and an analyzing unit (90) that measures the optical information of the sample (S) on the basis of the detected scattered light/fluorescence signals (SG2) and (SG3) and the detected transmission light signal (SG1) and analyzes the sample (S) on the basis of the measured optical information.
    • 光信息分析装置(10)包括对样本(S)发光(激发光)(L0)的发光单元(30),接收透射光(L1)的透射光接收单元(50) 通过样品(S)的激发光,并且检测作为透射光信号(SG1)的接收的透射光,设置在多个位置的散射光/荧光接收单元(60)和(70),接收侧 检测来自样品(S)的散射光/荧光成分(L2)和(L3),并检测作为散射光/荧光信号(SG2)和(SG3)的接收侧散射光/荧光成分,以及分析单元(90) 根据检测到的散射光/荧光信号(SG2)和(SG3)和检测到的透射光信号(SG1)来测量样品(S)的光学信息,并基于样品(S) 测量光信息。