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    • 1. 发明申请
    • A SIMPLE AND AFFORDABLE METHOD FOR IMMUNOPHENOTYPING USING A MICROFLUIDIC CHIP SAMPLE PREPARATION WITH IMAGE CYTOMETRY
    • 一种使用微型芯片样品制备与图像细胞计数进行免疫印迹的简单和可行的方法
    • US20140038230A1
    • 2014-02-06
    • US13817180
    • 2011-05-08
    • Markus BeckLeon L.W.M.M. Terstappen
    • Markus BeckLeon L.W.M.M. Terstappen
    • G01N33/50
    • G01N33/5094G01N1/28G01N15/1463G01N15/1484G01N33/56972G01N2015/008G01N2015/1486G01N2333/70514G01N2333/70517G01N2800/52G01N2800/56
    • The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and system disclosed herein largely overcome these limitations. The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm2 of blood (approximately 1 microliter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.
    • 通过流式细胞术对流体细胞的计数被广泛应用于许多学科,例如评估不同体液中的白细胞亚群或环境样品,食品和体液中的细菌污染。 对于许多应用,仪器的成本,尺寸和复杂性阻止了更广泛的使用,例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新型装置,方法和系统在很大程度上克服了这些限制。 该系统包括一个简单的CD4和CD8计数系统,在资源贫乏国家的护理点艾滋病毒分期。 与以前的方法不同,通过毛细管流将样品直接添加到含干燥试剂的芯片中,不需要样品制备。 使用由LED模块组成的大面积图像细胞仪来激发荧光染料PerCP和APC标记的靶标,并且具有两个宏观透镜的组合的单色CCD照相机捕获40mm 2血液(约1微升)的图像。 CD4和CD8-T淋巴细胞计数与通过流式细胞术获得的计数相关性良好。 在本发明中描述的细胞仪系统提供了一种在远程位置使用的经济实惠且易于使用的技术。
    • 3. 发明授权
    • Cable railway system
    • 有线铁路系统
    • US08522689B2
    • 2013-09-03
    • US13161025
    • 2011-06-15
    • Markus Beck
    • Markus Beck
    • B61B7/00B61B9/00
    • B61B12/105B61B12/022
    • A cableway system has a conveying cable that is guided via a head wheel pulleys in the two terminal stations of the system. Vehicles, such as gondolas or chairs, are coupled to the conveying cable between stations, and they are guided through the stations by way of travelling-gear mechanisms. In the stations, the vehicles are moved by way of tire wheels or control tires, and the tire wheels are driven by at least one supporting roller for the conveying cable. The supporting roller is located in the relevant station. The at least one of these supporting rollers for the conveying cable via which the drive power of the control tires is derived from the conveying cable is adjustable in the vertical direction.
    • 索道系统具有通过系统的两个终端站中的头轮滑轮引导的输送电缆。 诸如吊船或椅子的车辆联接到车站之间的输送缆线,并且它们通过行走齿轮机构被引导通过车站。 在车站中,车辆通过轮胎轮或控制轮胎移动,并且轮胎轮由用于输送缆线的至少一个支撑辊驱动。 支撑辊位于相关车站。 用于输送电缆的这些支撑辊中的至少一个可以通过其从输送电缆导出控制轮胎的驱动力在垂直方向上是可调节的。
    • 4. 发明授权
    • Obtaining sensing results indicating time variation
    • 获取指示时间变化的感测结果
    • US08153949B2
    • 2012-04-10
    • US12337737
    • 2008-12-18
    • Peter KieselJoerg MartiniMichael BasslerMarkus BeckNoble M. Johnson
    • Peter KieselJoerg MartiniMichael BasslerMarkus BeckNoble M. Johnson
    • G01J1/42F21V9/16G01N21/25
    • G01N15/1429G01N15/1056G01N15/12G01N15/1459G01N2015/1006G01N2015/1037G01N2015/1075
    • In response to objects having relative motion within an encoding/sensing region relative to an encoder/sensor that, e.g., photosenses emanating light or performs impedance-based sensing, sensing results can indicate sensed time-varying waveforms with information about the objects, about their relative motion, about excitation characteristics, about environmental characteristics, and so forth. An encoder/sensor can include, for example, a non-periodic arrangement of sensing elements; a longitudinal sequence of sensing elements with a combined sensing pattern that approximates a superposition or scaled superposition of simpler sensing patterns; and/or IC-implemented sensing elements that include photosensing arrays on ICs and readout/combine circuitry that reads out photosensed quantities from cells in groups in accordance with cell-group sensing patterns and combines the readout photosensed quantities to obtain the sensing results. Objects can move fluidically as in flow cytometry, through scanning movement as in document scanning, or in other ways.
    • 响应于相对于编码器/传感器在编码/感测区域内具有相对运动的物体,例如,发出光的光照或执行基于阻抗的感测,感测结果可以指示感测到的具有关于物体的信息的时变波形,关于它们 相对运动,关于激发特性,关于环境特性等。 编码器/传感器可以包括例如感测元件的非周期性布置; 具有组合感测图案的感测元件的纵向序列,其近似于更简单的感测图案的叠加或缩放叠加; 和/或IC实现的感测元件,其包括IC上的光敏阵列和读出/组合电路,其根据单元组感测图案从组中的单元读出光照量,并组合读出的光照量以获得感测结果。 物体可以像流式细胞仪一样流动地进行扫描运动,如文档扫描或其他方式。
    • 8. 发明申请
    • Transmitting/Reflecting Emanating Light With Time Variation
    • 发射/反射发光随时间变化
    • US20090195773A1
    • 2009-08-06
    • US12024490
    • 2008-02-01
    • Michael BasslerMarkus BeckPeter KieselAlex HegyiTobias BuergelNoble M. Johnson
    • Michael BasslerMarkus BeckPeter KieselAlex HegyiTobias BuergelNoble M. Johnson
    • G02B5/22G01N21/00
    • G01N21/05G01N21/255G01N21/645G01N2021/0346
    • A filter arrangement can transmit and/or reflect light emanating from a moving object so that the emanating light has time variation, and the time variation can include information about the object, such as its type. For example, emanating light from segments of a path can be transmitted/reflected through positions of a filter assembly, and the transmission functions of the positions can be sufficiently different that time variation occurs in the emanating light between segments. Or emanating light from a segment can be transmitted/reflected through a filter component in which simpler transmission functions are superimposed, so that time variation occurs in the emanating light in accordance with superposition of two simpler non-uniform transmission functions. Many filter arrangements could be used, e.g. the filter component could include the filter assembly, which can have one of the simpler non-uniform transmission functions. Time-varying waveforms from sensing results can be compared to obtain spectral differences.
    • 滤波器装置可以发射和/或反射从移动物体发出的光,使得发射光具有时间变化,并且时间变化可以包括关于物体的信息,例如其类型。 例如,可以通过路径的区段发出光,并通过过滤器组件的位置进行透射/反射,并且位置的透射函数可以充分地不同,从而在区间之间的发光中发生时间变化。 或者发射来自光束的光可以通过其中叠加更简单的透射函数的滤光器部件被透射/反射,使得根据两个更简单的不均匀透射函数的叠加在发射光中发生时间变化。 可以使用许多过滤装置,例如 过滤器组件可以包括过滤器组件,其可以具有更简单的不均匀传输功能之一。 可以比较感测结果中的时变波形,以获得光谱差异。
    • 9. 发明申请
    • Producing Time Variation In Emanating Light
    • 在发光时产生时间变化
    • US20090194705A1
    • 2009-08-06
    • US12023436
    • 2008-01-31
    • Peter KieselMarkus BeckMichael BasslerNoble M. Johnson
    • Peter KieselMarkus BeckMichael BasslerNoble M. Johnson
    • G01N21/64
    • G01N21/645G01N21/6486G01N2021/6419
    • An excitation component or arrangement can provide excitation to a moving object so that information is encoded in time variation of light emanating from the object. For example, in each of a sequence of segments, it can provide a respective non-binary excitation spectrum, and the spectra can be different with a non-interference-like transition between them; because the object emanates light differently in response to the different spectra, photosensing results can be obtained that include encoded information about the object. The non-binary spectra could be different intermediate intensities, such as different gray levels or different intensities of one color or could be different colors. The excitation can be provided in a pattern with non-interference-like transitions between regions, and object motion can also be controlled. In another approach, a trigger signal can cause a time-varying excitation in a region, with non-interference-like transitions between intervals of excitation, such as black/white, multiple colors, or gray levels.
    • 激励分量或排列可以向移动物体提供激励,使得信息在从对象发出的光的时间变化中被编码。 例如,在片段序列的每一个中,它可以提供相应的非二元激励光谱,并且光谱可以与它们之间的非干扰状的转变不同; 因为物体响应于不同的光谱不同地发出光,所以可以获得包括关于物体的编码信息的光敏结果。 非二进制光谱可以是不同的中间强度,例如不同的灰度级或一种颜色的不同强度,或者可以是不同的颜色。 激励可以以区域之间的非干涉状态的转变的模式提供,并且还可以控制物体运动。 在另一种方法中,触发信号可以在区域中引起时变激励,在激发间隔(例如黑/白,多种颜色或灰度级)之间具有非干扰状的过渡。