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1. 发明授权

US4852050A Signal processing method in autoradiography 失效
标题翻译：放射自显影中的信号处理方法
公开(公告)号：US4852050A
公开(公告)日：1989-07-25
申请号：US568907
申请日：1984-01-06
申请人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012 , Y10S250/909
摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph is then generated by recording and reproducing from a stimulable phosphor sheet the radiation energy stored therein. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on the support medium and/or synthesized from certain resolved rows. Sampling points in the remaining non-reference rows are identified by comparison with the continuous lines to thus determine the base sequence of the DNA or DNA fragment.
摘要翻译：放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分彼此平行地在支撑介质上形成分辨行。获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。然后通过从可刺激的荧光体片材记录和再现其中存储的辐射能量来生成对应于放射自显影仪的电数字信号。接下来检测数字信号的每个解决行中的采样点。然后在多个参考行中确定参考采样点，这些参考行直接设置在支持介质上和/或从某些已解决的行合成。通过与连续线进行比较来确定剩余非参考行中的采样点，从而确定DNA或DNA片段的碱基序列。

2. 发明授权

US4665312A Signal detecting method in autoradiography 失效
标题翻译：放射自显影中的信号检测方法
公开(公告)号：US4665312A
公开(公告)日：1987-05-12
申请号：US773858
申请日：1985-09-09
申请人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G03B42/02 , G01N27/26 , G01N27/447 , G01N33/50 , G01T1/29 , G01T1/20
CPC分类号： G01T1/2942 , G01T1/2014
摘要： A signal detecting method in autoradiography, which is applied to an autoradiography having locational information on radioactively labeled substances such as radioactively labeled biopolymers distributed one-dimensionally, being recorded on a stimulable phosphor sheet as a radiation energy-stored image, which comprises steps of:(1) preliminarily scanning a part of the stimulable phosphor sheet with an electromagnetic wave to release a portion of the radiation energy as stimulated emission and detecting the stimulated emission to give an electric signal, to which a signal processing is applied, to determine one-dimensional distribution direction of the radioactively labeled substances; and(2) finally scanning the stimulable phosphor sheet with an electromagnetic wave along said one-dimensional distribution direction to release the radiation energy as stimulated emission and detecting the stimulated emission, to obtain the locational information on the radioactively labeled substances as a digital signal.
摘要翻译：一种放射自显影中的信号检测方法，其应用于具有放射性标记物质的位置信息的放射自显影方法，所述放射自显影物质例如放射性标记的一维分布的生物聚合物作为辐射能量存储图像记录在可刺激的磷光体片上，其包括以下步骤：（1）用电磁波对一部分可刺激的荧光体片进行扫描，以释放辐射能量的一部分作为受激发射，并检测受激发射，给出施加信号处理的电信号，放射性标记物质的尺寸分布方向; 和（2）最后用沿着所述一维分布方向的电磁波扫描所述可刺激的荧光体片材，以释放作为受激发射的辐射能量并检测受激发射，以获得关于放射性标记物质的位置信息作为数字信号。

3. 发明授权

US4837733A Signal processing method in autoradiography 失效
标题翻译：放射自显影中的信号处理方法
公开(公告)号：US4837733A
公开(公告)日：1989-06-06
申请号：US883922
申请日：1986-07-10
申请人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012
摘要： A signal processing method in autoradiography for obtaining information on the one dimensional location of radioactively labeled substances in a sample where the substances of the sample are distributed in at least one row on a support medium where the information is in the form of symbols, numerals or a combination thereof. The method at least includes the steps of (1) generating digital image data where the data comprises sets of digital signals corresponding to the autoradiograph including the row of distributed substances and where the sets of digital signals may respectively represent successive linear portions of the autoradiograph; (2) scanning selected digital signals of the digital image data to generate at least one further signal representative of a predetermined portion of the row of distributed substances where the position of the predetermined portion is at least partially determined by its location in the scanning direction and the amount of radioactively labeled substance at the location is at least partially determined by the amplitude of the further signal; (3) processing the further signal with either or both of smoothing and threshold-processing to detect sampling point corresponding to the location of the predetermined portion of the row of distributed substances; (4) obtaining the image data digital corresponding to the autoradiograph having the locational information on the radioactively labeled substances where the digital image data is obtained by utilizing a radiation image recording and reproducing method of employing a stimulable phosphor sheet. A signal processing method for determining the base sequence of DNA is also disclosed.
摘要翻译：一种放射自显影中的信号处理方法，用于获得样品中放射性标记物质的一维位置的信息，其中样品的物质分布在支撑介质上的至少一行中，其中信息为符号，数字或数字的形式其组合。该方法至少包括以下步骤：（1）产生数字图像数据，其中数据包括对应于包括分布物质行的放射自显影仪的数字信号组，并且其中数字信号组可以分别表示放射自显影仪的连续线性部分; （2）扫描数字图像数据的所选择的数字信号以产生表示分布物质行的预定部分的至少一个另外的信号，其中预定部分的位置至少部分地由其在扫描方向上的位置确定; 位置处的放射性标记物质的量至少部分地由另外的信号的幅度决定; （3）用平滑和阈值处理中的任一个或两者来处理另外的信号，以检测对应于分配物质行的预定部分的位置的采样点; （4）获得与放射自显影仪对应的图像数据数字，该放射自显影仪具有放射性标记物质的位置信息，其中通过利用使用可刺激荧光粉片的放射线图像记录和再现方法获得数字图像数据。还公开了用于确定DNA的碱基序列的信号处理方法。

4. 发明授权

US4888695A Signal processing method in autoradiography 失效
标题翻译：放射自显影中的信号处理方法
公开(公告)号：US4888695A
公开(公告)日：1989-12-19
申请号：US568876
申请日：1984-01-06
申请人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29
CPC分类号： G01T1/2014 , G01T1/2012
摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph are generated by recording and reproducing a radiation image stored in a stimulable phosphor sheet. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on the support medium and/or synthesized from certain resolved rows. Corresponding reference sampling points in the plural reference rows are joined to generate plural continuous lines comprising straight lines, polygonal lines or curved lines. Sampling points in the remaining non-reference rows are identified by comparison with the continuous lines to thus determine the base sequence of the DNA or DNA fragment.
摘要翻译：放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分彼此平行地在支撑介质上形成分辨行。获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。通过记录和再现存储在可刺激的磷光体片中的放射线图像来生成与放射自显影仪相对应的电数字信号。接下来检测数字信号的每个解决行中的采样点。然后在多个参考行中确定参考采样点，这些参考行直接设置在支持介质上和/或从某些已解决的行合成。多个参考行中的相应参考采样点被连接以产生包括直线，多边形线或曲线的多个连续线。通过与连续线进行比较来确定剩余非参考行中的采样点，从而确定DNA或DNA片段的碱基序列。

5. 发明授权

US4777597A Signal processing method in autoradiography 失效
标题翻译：放射自显影中的信号处理方法
公开(公告)号：US4777597A
公开(公告)日：1988-10-11
申请号：US24909
申请日：1987-03-11
申请人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
发明人： Hisashi Shiraishi , Tsutomu Kimura , Kazuhiro Hishinuma
IPC分类号： G01T1/29 , G01N33/58 , C12Q1/68 , H04N1/40
CPC分类号： G01T1/2014 , G01T1/2012 , Y10S250/909
摘要： A signal processing method in autoradiography employing a stimulable phosphor sheet for the purpose of obtaining information on the one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of a symbol, numeral or combination thereof, including the steps of:(1) determining a one dimensional scanning line for signal processing; and(2) detecting sampling points on said scanning line,the process being applied to digital signals corresponding to an autoradiograph having the locational information on the radioactively labeled substances, the digital signals being obtained from stimulable phosphor sheet.
摘要翻译：为了获得关于在支撑介质上以至少一维方向分布的放射性标记的物质的一维位置的信息，以符号，数字或组合的形式，使用可激发的荧光体片的放射自显影的信号处理方法，包括以下步骤：（1）确定用于信号处理的一维扫描线; 和（2）检测所述扫描线上的采样点，该处理被应用于与具有关于放射性标记物质的位置信息的放射自显影仪对应的数字信号，数字信号是从可刺激的荧光粉片获得的。

6. 外观设计

USD361802S Toy loader set 失效
公开(公告)号：USD361802S
公开(公告)日：1995-08-29
申请号：US11993
申请日：1993-08-19
申请人： Hisashi Shiraishi
设计人： Hisashi Shiraishi

7. 发明授权

US4548870A Element for electrophoresis 失效
公开(公告)号：US4548870A
公开(公告)日：1985-10-22
申请号：US588751
申请日：1984-03-12
申请人： Masashi Ogawa , Hisashi Shiraishi , Teppei Ikeda
发明人： Masashi Ogawa , Hisashi Shiraishi , Teppei Ikeda
IPC分类号： G01N27/26 , B32B27/00 , G01N27/447 , B32B27/08 , C08L5/00 , C08L5/12 , G01N31/00
CPC分类号： G01N27/44747 , Y10T428/31507 , Y10T428/31623 , Y10T428/31728 , Y10T428/31743 , Y10T428/31746 , Y10T428/31754 , Y10T428/31783 , Y10T428/31797 , Y10T428/31971
摘要： An element for electrophoresis comprising the following three-layer structure laminated in the order:(I) a support layer;(II) an adhesive layer comprising a polymer having at least one specifically selected repeating unit;and(III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.

8. 发明授权

US4137082A Photographic light-sensitive material hardened with a vinylsulfonyl compound 失效
标题翻译：用乙烯基磺酰基化合物硬化的照相感光材料
公开(公告)号：US4137082A
公开(公告)日：1979-01-30
申请号：US837248
申请日：1977-09-27
申请人： Hidefumi Sera , Tsumoru Ishii , June Yamaguchi , Hisashi Shiraishi
发明人： Hidefumi Sera , Tsumoru Ishii , June Yamaguchi , Hisashi Shiraishi
IPC分类号： C07D251/04 , C07C67/00 , C07C313/00 , C07C317/44 , C07D295/185 , C08L89/00 , G03C1/30
CPC分类号： C07D295/185 , G03C1/30 , G03C1/305
摘要： A photographic silver halide light-sensitive material having at least one hydrophilic colloid layer containing (a) gelatin and/or a gelatin derivative and (b) at least one compound represented by the following general formula (I) or (II): ##STR1## wherein Y represents a vinyl group; A represents a divalent group; R, which may be the same or different, each represents a hydrogen atom or a hydrocarbon group having 1 to 4 carbon atoms; and n is 0 or 1. The compounds of the general formulas (I) and (II) are novel class of gelatin hardeners which are particularly suitable for use in silver halide photographic light-sensitive materials.
摘要翻译：具有至少一个含有（a）明胶和/或明胶衍生物的亲水胶体层的照相卤化银感光材料和（b）至少一种由以下通式（I）或（II）表示的化合物：

9. 发明授权

US5260190A Autoradiographic process 失效
标题翻译：放射自显影过程
公开(公告)号：US5260190A
公开(公告)日：1993-11-09
申请号：US378509
申请日：1989-07-06
申请人： Hisashi Shiraishi , Junji Miyahara , Hisatoyo Kato
发明人： Hisashi Shiraishi , Junji Miyahara , Hisatoyo Kato
IPC分类号： G01T1/29 , C12Q1/68 , G01N33/566 , G03C5/16
CPC分类号： G01T1/2014 , G01T1/2012 , Y10T436/143333
摘要： An autoradiographic process for obtaining information on one or two dimensional location of radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances such as DNA originating from an organism,which comprises:placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers for a certain period to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;scanning said phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet as stimulated emission; anddetecting the stimulated emission to obtain locational information on the radioactively labeled substances in the sample.
摘要翻译：一种放射自显影方法，用于获得选自以下的样品中所含的放射性标记物质的一维或二维位置的信息：生物体组织和含生物体组织的培养基和/或源自生物的DNA的物质，其包括：将所述样品和可激发的荧光体片材具有包含分散在粘合剂中的可刺激磷光体的荧光体层一起分层一定时间，以使所述荧光体片材吸收由放射性标记的物质发射的辐射能量的至少一部分在所述样品中; 用电磁波扫描所述荧光粉片，以释放存储在所述荧光粉片中的辐射能量的至少一部分作为受激发射; 并检测受激发射以获得样品中放射性标记物质的位置信息。

10. 发明授权

US4699680A Process and apparatus for manufacturing element for electrophoresis 失效
标题翻译：制造电泳元件的方法和装置
公开(公告)号：US4699680A
公开(公告)日：1987-10-13
申请号：US716952
申请日：1985-03-28
申请人： Hisashi Shiraishi , Mineo Suyefuji , Masashi Kato
发明人： Hisashi Shiraishi , Mineo Suyefuji , Masashi Kato
IPC分类号： G01N27/26 , G01N27/447 , B29C39/00 , B32B31/18
CPC分类号： G01N27/44704 , Y10T156/108 , Y10T156/12
摘要： A process of manufacturing an element for electrophotresis which comprises steps of: sticking a spacer continuously to both side portions of a support web which is rolled back continuously from the rolled condition; casting on said support a solution for forming a medium membrane for electrophoresis; subjecting the support to hardening treatment to form a medium membrane; cutting off a portion of the medium membrane and support web; and sticking a cover sheet onto the medium membrane. An apparatus advantageously employable for performing the above process is disclosed.
摘要翻译：一种用于电泳的元件的制造方法，包括以下步骤：将间隔物连续地粘贴到从轧制状态连续回退的支撑纤维网的两侧部分; 在所述载体上浇铸形成用于电泳的介质膜的溶液; 对载体进行硬化处理以形成介质膜; 切断介质膜的一部分和支撑纤维网; 并将覆盖片粘贴到介质膜上。公开了一种有利地用于执行上述过程的设备。

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