专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07432046B2 Methods for the determination of antibody IgG avidity 有权
标题翻译：测定抗体IgG亲合力的方法
公开(公告)号：US07432046B2
公开(公告)日：2008-10-07
申请号：US11265481
申请日：2005-11-02
申请人： Gregory T. Maine , Stephen C. Hsu , Darwin D. Smith , Dominick L. Pucci , Jörg Herzogenrath , Ingo Curdt , Heike Maria Christ
发明人： Gregory T. Maine , Stephen C. Hsu , Darwin D. Smith , Dominick L. Pucci , Jörg Herzogenrath , Ingo Curdt , Heike Maria Christ
IPC分类号： G01N33/543 , G01N33/553 , G01N33/552 , G01N33/544 , G01N33/546 , G01N33/53 , G01N33/00 , C12Q1/70
CPC分类号： G01N33/56905 , G01N33/56994 , G01N33/6854 , G01N2333/45
摘要： The present invention relates to methods of determining anti-infectious agent IgG avidity, for example, human anti-cytomegalovirus and human anti-toxoplasma IgG avidity, using a competitive assay format.
摘要翻译：本发明涉及使用竞争性测定形式测定抗感染剂IgG亲合力的方法，例如人抗巨细胞病毒和人抗弓形体IgG亲合力。

2. 发明授权

US07094879B2 Genetically engineered P30 antigen, improved antigen cocktail, and uses thereof 有权
标题翻译：遗传工程化的P30抗原，改良抗原鸡尾酒及其用途
公开(公告)号：US07094879B2
公开(公告)日：2006-08-22
申请号：US10263153
申请日：2002-10-02
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00 , G01N33/53
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

3. 发明授权

US5786181A Process for producing high purity 6, 12-dideoxyerythromycin A by fermentation 失效
标题翻译：通过发酵生产高纯度6,12-脱红霉素A的方法
公开(公告)号：US5786181A
公开(公告)日：1998-07-28
申请号：US789979
申请日：1997-01-28
申请人： Diane L. Stassi , Gregory T. Maine , David A. Post , Mark T. Satter
发明人： Diane L. Stassi , Gregory T. Maine , David A. Post , Mark T. Satter
IPC分类号： C12N15/09 , C12N5/10 , C12N9/06 , C12N9/10 , C12N15/76 , C12N15/90 , C12P19/62
CPC分类号： C12N9/0012 , C12N15/76 , C12N15/90 , C12N9/1007 , C12P19/62
摘要： A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.
摘要翻译：公开了使用重组DNA技术生产高纯度6,12-二脱氧红霉素A的方法。缺乏红霉素C-12和C-6羟化酶的红霉素产生菌，红酵母，产生6,12-脱羟基红霉素A和前体分子6-脱氧红霉素D的混合物。为了使前体转化成最终产物，将eryG的第二个拷贝插入Sac的非必需区域。导致红细胞染色体高纯度6,12-脱氧红霉素A.

4. 发明授权

US07314924B2 Polynucleotide encoding a genetically engineered P30 antigen 有权
标题翻译：编码遗传工程化的P30抗原的多核苷酸
公开(公告)号：US07314924B2
公开(公告)日：2008-01-01
申请号：US11316532
申请日：2005-12-21
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/74 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

5. 发明授权

US5024941A Expression and secretion vector for yeast containing a glucoamylase signal sequence 失效
标题翻译：含有葡糖淀粉酶信号序列的酵母的表达和分泌载体
公开(公告)号：US5024941A
公开(公告)日：1991-06-18
申请号：US810423
申请日：1985-12-18
申请人： Gregory T. Maine , Robert S. Daves , Robert R. Yocum
发明人： Gregory T. Maine , Robert S. Daves , Robert R. Yocum
IPC分类号： C12N15/09 , C12N1/16 , C12N9/26 , C12N9/34 , C12N15/00 , C12P21/00 , C12Q1/04 , C12Q1/14 , C12R1/865
CPC分类号： C12N9/2428 , C12Q1/04 , C12Q1/14 , C07K2319/02
摘要： A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae; and upstream from the signal-encoding sequence, a DNA sequence capable of promoting transcription in yeast (e.g., a high yield promoter, such as the promoter of the triose phosphate isomerase gene), transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence. The vector is useful as an expression vector in yeast.
摘要翻译：一种载体，其包含编码与来自糖酵母或酿酒酵母的葡糖淀粉酶基因的分泌信号编码序列基本相同的分泌信号序列的DNA序列; 和信号编码序列的上游，能够在酵母中促进转录的DNA序列（例如，高产率启动子，例如磷酸丙糖异构酶基因的启动子），信号编码序列的转录在转录促进序列，用于插入异源DNA序列的载体的位点，在具有信号编码序列的阅读框中。该载体可用作酵母中的表达载体。

6. 发明授权

US07824908B2 Genetically engineered P30 antigen, improved antigen cocktail, and uses thereof 有权
标题翻译：遗传工程化的P30抗原，改良抗原鸡尾酒及其用途
公开(公告)号：US07824908B2
公开(公告)日：2010-11-02
申请号：US11860383
申请日：2007-09-24
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

7. 发明授权

US06287760B1 Western blot test for the identification of antibodies specific against the HCMV virus 有权
标题翻译：用于鉴定针对HCMV病毒的抗体的蛋白质印迹测试
公开(公告)号：US06287760B1
公开(公告)日：2001-09-11
申请号：US09242391
申请日：1999-07-08
申请人： Maria P. Landini , Gregory T. Maine , Alessandro Ripalti , Tiziana Lazzarotto
发明人： Maria P. Landini , Gregory T. Maine , Alessandro Ripalti , Tiziana Lazzarotto
IPC分类号： C12Q170
CPC分类号： G01N33/56994 , C07K14/005 , C07K2319/00 , C12N2710/16122 , G01N33/543 , G01N33/54386 , G01N33/559 , G01N2333/045 , G01N2469/20
摘要： Diagnostic tool for the identification of anti-HCMV antibodies in human serum. The tool comprises a means of solid support having two sections, a first section bearing a plurality of HCMV proteins (vp) obtained from purified virions concentrated in separate bands forming a predetermined pattern, one of these bands being pp 150 of HCMV; a second section bearing recombinant fusion proteins as controls, at least one band comprising a recombinant fusion protein carrying at least one epitope of pp 150.
摘要翻译：用于鉴定人血清中抗HCMV抗体的诊断工具。该工具包括具有两个部分的固体支持装置，第一部分带有多个HCMV蛋白（vp），其从浓缩在形成预定图案的分离带中的纯化病毒体获得，这些条带中的一个是HCMV的pp 150; 第二部分，其携带重组融合蛋白作为对照，至少一个带包含携带至少一个pp 150的表位的重组融合蛋白。

8. 发明申请

US20080160606A1 GENETICALLY ENGINEERED P30 ANTIGEN, IMPROVED ANTIGEN COCKTAIL, AND USES THEREOF 有权
标题翻译：遗传工程P30抗原，改良的抗原蛋白及其用途
公开(公告)号：US20080160606A1
公开(公告)日：2008-07-03
申请号：US11860383
申请日：2007-09-24
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

9. 发明授权

US06177241B1 Use of peptides to improve specificity of an immunoassay for the detection of cytomegalovirus specific IgM antibody 失效
标题翻译：使用肽来提高检测巨细胞病毒特异性IgM抗体的免疫测定的特异性
公开(公告)号：US06177241B1
公开(公告)日：2001-01-23
申请号：US08935009
申请日：1997-09-22
申请人： Gregory T. Maine
发明人： Gregory T. Maine
IPC分类号： C12Q170
CPC分类号： G01N33/56994
摘要： This invention provides a method, a reagent, and a kit for detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The invention also provides a reagent for use in detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The method of this invention comprises the steps of: (a) providing a solid phase containing at least a portion of a herpesvirus antigen; (b) introducing a patient specimen to the solid phase of step (a); (c) introducing at least one peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase of step (a); d) allowing IgM antibodies to herpesvirus to specifically bind to the portion of antigen of herpesvirus and the peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase; and (e) determining the level of IgM antibodies to herpesvirus in the patient specimen.
摘要翻译：本发明提供了一种用于检测指示最近感染的疱疹病毒特异性IgM抗体的方法，试剂和试剂盒，同时防止存在于低风险个体中的低水平疱疹病毒特异性IgM抗体。本发明还提供了用于检测指示最近感染的疱疹病毒特异性IgM抗体的试剂，同时防止检测低风险个体中存在的低水平疱疹病毒特异性IgM抗体。本发明的方法包括以下步骤：（a ）提供含有至少一部分疱疹病毒抗原的固相;（b）将患者标本导入步骤（a）的固相;（c）将至少一种能够将IgM抗体特异性结合疱疹病毒的肽引入到步骤（a）的固相; d）允许疱疹病毒的IgM抗体特异性结合疱疹病毒抗原部分和能够将IgM抗体与疱疹病毒特异性结合至固相的肽; 和（e）确定患者标本中疱疹病毒IgM抗体的水平。

10. 发明授权

US6074817A Recombinant mono and poly antigens to detect cytomegalovirus-specific IgM in human sera by enzyme immunoassay 失效
标题翻译：通过酶免疫测定法检测人血清中巨细胞病毒特异性IgM的重组单抗和多抗原
公开(公告)号：US6074817A
公开(公告)日：2000-06-13
申请号：US765856
申请日：1996-12-27
申请人： Maria P. Landini , Alessandro Ripalti , Gregory T. Maine , Richard T. Flanders
发明人： Maria P. Landini , Alessandro Ripalti , Gregory T. Maine , Richard T. Flanders
IPC分类号： G01N33/53 , C07K14/045 , C12N1/21 , C12N15/09 , C12N15/38 , C12P21/02 , G01N33/569 , G01N33/577 , C12Q1/70
CPC分类号： C07K14/005 , C07K2319/00 , C12N2710/16122
摘要： A mixture of recombinant mono- and poly-epitope proteic materials able to fully replace the viral antigens when used in an enzyme immunoassay (EIA) is disclosed; the mixture includes a poly-epitope fusion protein having a first region formed by an amino acid sequence (H10) corresponding to that of the last 233 amino acids of the COOH terminus of the viral protein p52 or to a part thereof, a second region formed by an amino acid sequence (F3) corresponding to that of the last 43 amino acids of the COOH terminus of viral protein pp150 or to a part thereof, and a third region formed by an amino acid sequence (A1C2) corresponding to that taken from aa 595 to aa 614, proceeding in direction 5'.fwdarw.3', of the same viral protein pp150; and, in combination, a second fusion protein including a sequence of amino acids corresponding to that taken, proceeding in direction 5'.fwdarw.3', from aa 297 to aa 510 of the viral major matrix protein pp65 encoded by the viral gene UL83 and a third fusion protein including a sequence of amino acids corresponding to that taken, proceeding in direction 5'.fwdarw.3', from aa 117 to aa 373 of the viral assembly protein pp38 encoded by the viral gene UL80a. These three fusion proteins may be used combined together for the preparation of an ELISA test kit for detection of Cytomegalovirus-specific IgM in human sera.
摘要翻译： PCT No.PCT / IT95 / 00073 Sec。 371日期1996年12月27日第 102（e）日期1996年12月27日PCT提交1995年5月15日PCT公布。公开号WO96 / 0132201。日期1996年1月18日公开了当用于酶免疫测定（EIA）时能够完全替代病毒抗原的重组单 - 和多表位蛋白质材料的混合物。该混合物包括多表位融合蛋白，其具有由与病毒蛋白p52的COOH末端的最后233个氨基酸相对应的氨基酸序列（H10）或其一部分形成的第一区域，形成第二区域通过对应于病毒蛋白pp150的COOH末端的最后43个氨基酸的氨基酸序列（F3）或其一部分的氨基酸序列（F3）和由与aa相对应的氨基酸序列（A1C2）形成的第三区域 595至aa 614，进行相同病毒蛋白pp150的方向5'→3'; 并且组合地包含由病毒基因UL83编码的病毒主要基质蛋白pp65的297至aa 510的方向5'→3'所对应的氨基酸序列的第二融合蛋白，以及第三融合蛋白，其包含对应于由病毒基因UL80a编码的病毒组装蛋白pp38的aa 117至a 373的方向5'→3'所对应的氨基酸序列。这三种融合蛋白可以结合在一起用于制备用于检测人血清中巨细胞病毒特异性IgM的ELISA测试试剂盒。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式