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1. 发明申请

WO2013078019A1 THREE DIMENSIONAL MATRIX ANALYSES FOR HIGH THROUGHPUT SEQUENCING 审中-公开
标题翻译：用于高通量序列的三维矩阵分析
公开(公告)号：WO2013078019A1
公开(公告)日：2013-05-30
申请号：PCT/US2012/064306
申请日：2012-11-09
申请人： DOW AGROSCIENCES LLC
发明人： GERMAN, Marcelo Ariel , LIU, Xing Liang , NOVAK, Stephen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6855 , C12Q1/6869 , C12Q2521/501 , C12Q2525/191 , C12Q2533/101 , C12Q2535/122 , C12Q2537/143 , C12Q2537/165 , C12Q2563/155 , C12Q2563/179
摘要： Methods and systems for combining a multi-dimensional matrix (with at least three dimensions) and high-throughput sequencing technologies to identify/recover genomic locations of each insert in thousands of transgenic plants simultaneously. In some embodiments, multiplex sequencing is carried out and sequencing data are imported in parallel into sequence data base for displaying in the multi-dimensional matrix.
摘要翻译：用于组合多维矩阵（至少三维）和高通量测序技术以同时识别/恢复数千个转基因植物中每个插入片段的基因组位置的方法和系统。在一些实施例中，执行多路测序，并且将测序数据并行导入到用于在多维矩阵中显示的序列数据库。

2. 发明申请

WO2009042164A1 ENGINEERED ZINC FINGER PROTEINS TARGETING 5-ENOLPYRUVYL SHIKIMATE-3-PHOSPHATE SYNTHASE GENES 审中-公开
标题翻译：工程化锌指纹蛋白靶向5-enolPYRUVYL SHIKIMATE-3-PHOSPHATE SYNTHASE GENES
公开(公告)号：WO2009042164A1
公开(公告)日：2009-04-02
申请号：PCT/US2008/011089
申请日：2008-09-25
申请人： DOW AGROSCIENCES LLC , SANGAMO BIOSCIENCES, INC. , GUPTA, Manju , PALTA, Asha, M. , NOVAK, Stephen , URNOV, Fyodor , GOPALAN, Sunita
发明人： GUPTA, Manju , PALTA, Asha, M. , NOVAK, Stephen , URNOV, Fyodor , GOPALAN, Sunita
IPC分类号： C12N15/82 , C12N9/22 , C12N15/90 , A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C07K2319/81 , C12N9/1092 , C12N15/8243 , C12N15/8274
摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.
摘要翻译：本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

3. 发明申请

WO2013184764A2 CONSTRUCT AND METHOD FOR EXPRESSING TRANSGENES USING A BRASSICA BIDIRECTIONAL CONSTITUTIVE PROMOTER 审中-公开
标题翻译：使用BRASSICA双向构成促进剂表达转基因的构造和方法
公开(公告)号：WO2013184764A2
公开(公告)日：2013-12-12
申请号：PCT/US2013/044262
申请日：2013-06-05
申请人： DOW AGROSCIENCES LLC
发明人： GUPTA, Manju , RUSSELL, Sean Michael , SHEN, Liu Yin , CHENNAREDDY, Sivarama Reddy , ROUT, Jyoti R , NOVAK, Stephen
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8216
摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from Brassica napus or Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.
摘要翻译：提供了使用公开的来自甘蓝型油菜或芸苔属双侧组成型启动子（BBCP）的双向启动子在植物细胞和/或植物组织中表达多个基因的构建体和方法。提供的构建体包含至少一个与多个基因表达盒连接的双向启动子，其中每个基因表达盒包含至少一个转基因。在一些实施方案中，提供的构建体和方法允许在二至二十个之间表达基因。

4. 发明申请

WO2012138549A1 HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS 审中-公开
标题翻译：转基因边界的高通量分析
公开(公告)号：WO2012138549A1
公开(公告)日：2012-10-11
申请号：PCT/US2012/031334
申请日：2012-03-30
申请人： DOW AGROSCIENCES LLC , CAO, Zehui , NOVAK, Stephen , ZHOU, Ning
发明人： CAO, Zehui , NOVAK, Stephen , ZHOU, Ning
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6855 , C12Q1/6895 , C12Q2525/155 , C12Q2525/191 , C12Q2549/119 , C12Q2565/519
摘要： The present invention is a method to identify unknown DNA sequences which flank known DNA sequences. The invention improves the accuracy, sensitivity, and reproducibility for determining unknown DNA sequences which flank a known DNA sequence. This claimed method can be deployed as a high throughput method to quickly and efficiently identify plant genomic chromosomal sequences which flank a transgene. Further analysis of these unknown sequences can be used to characterize the transgene insertion site for the identification of rearrangements, insertions and deletions which result from the integration of the transgene. In addition, analysis of the chromosomal flanking sequences can be used to identify the location of the transgene on the chromosome.
摘要翻译：本发明是识别与已知DNA序列相邻的未知DNA序列的方法。本发明改进了用于确定侧翼于已知DNA序列的未知DNA序列的准确度，灵敏度和重现性。这种所要求的方法可以作为高通量方法来部署，以快速有效地鉴定侧基于转基因的植物基因组染色体序列。这些未知序列的进一步分析可用于表征转基因插入位点，用于鉴定由转基因整合产生的重排，插入和缺失。此外，染色体侧翼序列的分析可用于鉴定转基因在染色体上的位置。

5. 发明申请

WO2011049627A1 ENGINEERED ZINC FINGER PROTEINS TARGETING PLANT GENES INVOLVED IN FATTY ACID BIOSYNTHESIS 审中-公开
标题翻译：工程化锌指纹蛋白质涉及脂肪酸生物合成中涉及的植物基因
公开(公告)号：WO2011049627A1
公开(公告)日：2011-04-28
申请号：PCT/US2010/002817
申请日：2010-10-22
申请人： DOW AGROSCIENCES LLC , SANGAMO BIOSCIENCES, INC. , DEKELVER, Russell , GUPTA, Manju , MILLER, Jeffrey C. , NOVAK, Stephen , PETOLINO, Joseph F.
发明人： DEKELVER, Russell , GUPTA, Manju , MILLER, Jeffrey C. , NOVAK, Stephen , PETOLINO, Joseph F.
IPC分类号： C12N15/82 , C12N5/04 , A01H5/00
CPC分类号： C12N15/8218 , C07K14/415 , C12N9/22 , C12N15/8216 , C12N15/8247 , C12Y203/01041 , C12Y301/00
摘要： The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
摘要翻译：本公开涉及靶向参与脂肪酸生物合成的植物中的基因的工程化锌指蛋白。还提供了使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。

6. 发明申请

WO2018111640A1 RECONSTRUCTION OF SITE SPECIFIC NUCLEASE BINDING SITES 审中-公开
公开(公告)号：WO2018111640A1
公开(公告)日：2018-06-21
申请号：PCT/US2017/064838
申请日：2017-12-06
申请人： DOW AGROSCIENCES LLC
发明人： CORBIN, David H. , CHEN, Wei , NOVAK, Stephen , LEE, Ryan M. , KUMAR, Sandeep , ASBERRY, Andrew , WORDEN, Andrew F.
IPC分类号： C12N15/00 , C12N15/90
摘要： Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

7. 发明申请

WO2014039872A1 ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING 审中-公开
标题翻译：用于基因定位和路由堆栈的工程变换集成平台（ETIP）
公开(公告)号：WO2014039872A1
公开(公告)日：2014-03-13
申请号：PCT/US2013/058584
申请日：2013-09-06
申请人： DOW AGROSCIENCES LLC
发明人： COGAN, Noel , FORSTER, John , HAYDEN, Matthew , SAWBRIDGE, Tim , SPANGENBERG, German , WEBB, Steven R. , GUPTA, Manju , AINLEY, W. Mike , HENRY, Matthew J. , MASON, John , KUMAR, Sandeep , NOVAK, Stephen
IPC分类号： C12N15/82 , A01H5/00 , C12N15/29
CPC分类号： C12N15/8241 , C12N15/8213
摘要： An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3' ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.
摘要翻译：描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测。主题披露中的一个要素是在ETIP内引入特定的双链断裂。在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。

8. 发明申请

WO2011066360A1 DETECTION OF AAD-12 SOYBEAN EVENT 416 审中-公开
标题翻译：检测AAD-12大豆事件416
公开(公告)号：WO2011066360A1
公开(公告)日：2011-06-03
申请号：PCT/US2010/057967
申请日：2010-11-24
申请人： DOW AGROSCIENCES LLC , NOVAK, Stephen , CUI, Yunxing Cory , GREENE, Thomas W. , ZHOU, Ning
发明人： NOVAK, Stephen , CUI, Yunxing Cory , GREENE, Thomas W. , ZHOU, Ning
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C12N15/82
CPC分类号： C12N15/8274 , C12Q1/6895 , C12Q2600/16
摘要： The invention relates in part to methods of detecting an AAD- 12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD- 12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event / polynucleotide sequence can be "stacked" with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.
摘要翻译：本发明部分涉及检测AAD-12大豆事件的方法。本发明提供了用于检测样品（例如大豆）的受试者事件的存在的测定。还提供了用于进行测定的试剂盒和条件。更具体地，本发明部分涉及AAD-12大豆事件的终点TaqMan PCR测定。一些实施方案涉及能够进行高通量接合度分析的测定。本发明还部分涉及用于确定接合性的优选参考基因的发现。本发明还部分地涉及使用任何本发明方法的植物育种。在一些实施方案中，所述事件/多核苷酸序列可以与其他特征“堆叠”。本发明方法可用于唯一地鉴定包含本发明事件的大豆品系。

9. 发明申请

WO2017222779A1 METHODOLOGIES AND COMPOSITIONS FOR CREATING TARGETED RECOMBINATION AND BREAKING LINKAGE BETWEEN TRAITS 审中-公开
标题翻译：用于创建目标重组和破坏特性的方法和组合
公开(公告)号：WO2017222779A1
公开(公告)日：2017-12-28
申请号：PCT/US2017/035605
申请日：2017-06-02
申请人： DOW AGROSCIENCES LLC
发明人： KUMAR, Sandeep , WORDEN, Andrew F. , NOVAK, Stephen , LEE, Ryan M.
IPC分类号： C12N15/05 , C12N15/82
摘要： The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.
摘要翻译：本公开涉及通过植物育种和植物遗传学改良植物品种的方法和组合物。例如，本公开涉及增加与植物内第二性状遗传连锁的杂合性状的重组频率。此外，本公开涉及打破第一等位基因和第二等位基因之间的遗传连锁。

10. 发明申请

WO2013078318A1 HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS 审中-公开
标题翻译：转基因边界的高通量分析
公开(公告)号：WO2013078318A1
公开(公告)日：2013-05-30
申请号：PCT/US2012/066267
申请日：2012-11-21
申请人： DOW AGROSCIENCES LLC
发明人： CAO, Zehui , NOVAK, Stephen , SASTRY-DENT, Lakshmi , ZHOU, Ning
IPC分类号： C40B20/00 , C40B30/00 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6895
摘要： A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.
摘要翻译：在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和通过链霉亲和素 - 生物素相互作用引物延伸反应产物。通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。

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