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2. 发明申请

WO2003048329A3 CHROMOSOMAL SATURATION MUTAGENESIS 审中-公开
公开(公告)号：WO2003048329A3
公开(公告)日：2003-06-12
申请号：PCT/US2002/038587
申请日：2002-12-03
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , CAYOUETTE, Michelle
发明人： SHORT, Jay, M. , CAYOUETTE, Michelle
IPC分类号： C12Q1/68
摘要： In One aspect, the invention provides methods for Chromosomal Saturation Mutagenesis (CSM) comprising generating randomly mutated, overlapping segments for an entire chromosome using error-prone PCR or other techniques, and libraries of nucleic acids made by these methods.

3. 发明申请

WO2003027315A3 AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
公开(公告)号：WO2003027315A3
公开(公告)日：2003-04-03
申请号：PCT/US2002/031010
申请日：2002-09-27
申请人： DIVERSA CORPORATION , ROBERTSON, Dan , MURPHY, Dennis , MATHUR, Eric, J. , SHORT, Jay, M.
发明人： ROBERTSON, Dan , MURPHY, Dennis , MATHUR, Eric, J. , SHORT, Jay, M.
IPC分类号： C12N9/78
摘要： The invention provides enzymes having an amidase activity, for example, catalyzing the removal of arginine, phenylalanine or methionine from the N-terminal end of polypeptides or peptides, e.g., in peptide or peptidomimetic synthesis. Amidase activities of the enzymes of the invention include deamidating peptide amides or N-terminally protected amino acids, the racemate splitting of N-protected amino acid amides, and for converting amides to carboxylic acids. In one aspect, an enzyme of the invention specifically acts on an (S)-amide; thus, it can be used to stereospecifically convert a mixture of (R)- and (S)-amides to the corresponding enantiomeric (S)-carboxylic acid.

4. 发明申请

WO2003002066A3 GLYCOSYLATED KINAMYCINS AND METHODS OF MAKING AND USING THEM 审中-公开
公开(公告)号：WO2003002066A3
公开(公告)日：2003-01-09
申请号：PCT/US2002/020719
申请日：2002-06-27
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , PARADKAR, Ashish , VAROGLU, Mustafa , MATHUR, Eric, J.
发明人： SHORT, Jay, M. , PARADKAR, Ashish , VAROGLU, Mustafa , MATHUR, Eric, J.
IPC分类号： C12N15/00
摘要： [00138] The invention provides compositions, including pharmaceuticals, comprising glycosylated kinamycins. The compositions and methods of the invention can be used to treat infections, i.e., as antibiotics, and as anti-tumor agents. The compositions of the invention can also act as electrophilic azo-coupling agents in vitro or vivo. The invention also provides enzymes capables of generating kinamycin, nucleic acids that encode them, antibodies that bind to them, and methods for making and using them.

5. 发明申请

WO2002068589A3 ENZYMES HAVING ALPHA AMYLASE ACTIVITY AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：WO2002068589A3
公开(公告)日：2002-09-06
申请号：PCT/US2002/005068
申请日：2002-02-21
申请人： DIVERSA CORPORATION , CALLEN, Walter , RICHARDSON, Toby , FREY, Gerhard , SHORT, Jay, M. , KEROVUO, Janne, S. , MATHUR, Eric, J. , SLUPSKA, Matgorzata , GRAY, Kevin
发明人： CALLEN, Walter , RICHARDSON, Toby , FREY, Gerhard , SHORT, Jay, M. , KEROVUO, Janne, S. , MATHUR, Eric, J. , SLUPSKA, Matgorzata , GRAY, Kevin
IPC分类号： C12P21/06
摘要： The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, and alkaline pH and increased temperature.

6. 发明申请

WO2002068583A3 ENZYMES HAVING DEHALOGENASE ACTIVITY AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：WO2002068583A3
公开(公告)日：2002-09-06
申请号：PCT/US2001/045337
申请日：2001-11-30
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , MATHUR, Eric, J. , RICHARDSON, Toby , ROBERTSON, Dan , GRAY, Kevin
发明人： SHORT, Jay, M. , MATHUR, Eric, J. , RICHARDSON, Toby , ROBERTSON, Dan , GRAY, Kevin
IPC分类号： C12Q1/68
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.

7. 发明申请

WO2004035729A2 ESTERASES, NUCLEIC ACIDS ENCODING THEM AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译： ESTERASES，NUCLEIC ACID ENCODING THEM AND METHODS OF MAKING AND USING THEM
公开(公告)号：WO2004035729A2
公开(公告)日：2004-04-29
申请号：PCT/US2002/022336
申请日：2002-07-10
申请人： DIVERSA CORPORATION , ROBERTSON, Dan , MURPHY, Dennis , REID, John , MAFFIA, Anthony, III. , LINK, Steven , SWANSON, Ronald, V. , WARREN, Patrick, V. , LENOX, Anna , SHORT, Jay, M. , MATHUR, Eric, J.
发明人： ROBERTSON, Dan , MURPHY, Dennis , REID, John , MAFFIA, Anthony, III. , LINK, Steven , SWANSON, Ronald, V. , WARREN, Patrick, V. , LENOX, Anna , SHORT, Jay, M. , MATHUR, Eric, J.
IPC分类号： C12N
CPC分类号： C12N9/18 , A01K2217/05 , A61K38/00 , C12N9/16 , C12P13/04
摘要： The invention is directed to enzymes having an esterase activity, including thermostable and thermotolerant esterase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The esterase activities of the enzymes of the invention include lipase activity (in the hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), in ester synthesis, and ester interchange reactions. The enzymes of the invention are used in organic synthesis reactions in the manufacture of medicaments, pesticides or intermediates thereof.
摘要翻译：本发明涉及具有酯酶活性的酶，包括耐热和耐热酯酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的酶的酯酶活性包括脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化的脂肪酸），酯交换反应（甘油三酯之间的脂肪酸交换），酯合成和酯交换反应。本发明的酶用于制备药物，农药或其中间体的有机合成反应中。

8. 发明申请

WO2003031928A2 CELLULAR ENGINEERING, PROTEIN EXPRESSION PROFILING, DIFFERENTIAL LABELING OF PEPTIDES, AND NOVEL REAGENTS THEREFOR 审中-公开
标题翻译：细胞工程，蛋白质表达谱，肽差异标记及其新型试剂
公开(公告)号：WO2003031928A2
公开(公告)日：2003-04-17
申请号：PCT/US2002/023549
申请日：2002-07-22
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
发明人： SHORT, Jay, M. , LATTERICH, Martin , WEI, Jing , LEVIN, Michael
IPC分类号： G01N
CPC分类号： G01N33/6848 , G01N33/6818 , G01N2458/15
摘要： The invention provides cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. Furthermore, a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products. Additionally, the invention provides novel methods for determining polypeptide profiles, and protein expression variations, which methods of simultaneously identifying and quantifying individual proteins in complex protein mixtures. Protein expression levels can be globally quantified.
摘要翻译：本发明提供了用于产生具有所需性质的新型转基因生物的细胞转化，定向进化和筛选方法。因此，在一个方面，本发明涉及产生具有差异可激活的多种性状的转基因生物体如微生物或植物的方法。此外，通过引入可在预期宿主中操作的调节序列（例如启动子）来重新组合基因和基因途径的方法，从而在新基因途径被引入预期宿主时赋予其可操作性。例如，基于微生物来源的祖细胞模板产生的新型人造基因途径，其可在植物细胞中操作。此外，产生具有所需性状，重组基因和基因产物的表达增加的新宿主生物的方法。另外，本发明提供了确定多肽概况和蛋白质表达变化的新方法，该方法同时鉴定和定量复合蛋白质混合物中的各种蛋白质。可以全面量化蛋白质表达水平。

9. 发明申请

WO2003023029A1 POLYMERASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：聚合物，编码它们的核酸及其制备和使用它们的方法
公开(公告)号：WO2003023029A1
公开(公告)日：2003-03-20
申请号：PCT/US2002/028464
申请日：2002-09-06
申请人： DIVERSA CORPORATION , CALLEN, Walter , MATHUR, Eric, J. , SHORT, Jay, M.
发明人： CALLEN, Walter , MATHUR, Eric, J. , SHORT, Jay, M.
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , A01K2217/05
摘要： The invention provides polymerases, nucleic acids that encode them and methods for making and using them. The polymerase enzymes of the invention can have different polymerase activities at various high temperatures. In one aspect, the polymerase activity comprises addition of deoxynucleotides at the 3' hydroxyl end of a polynucleotide. The invention also provides kits, e.g. , diagnostic kits, and methods for performing various amplification reactions, e.g. , polymerase chain reactions, transcription amplifications, ligase chain reactions self-sustained sequence replication or Q Beta replicase amplifications.
摘要翻译：本发明提供了聚合酶，编码它们的核酸以及制备和使用它们的方法。本发明的聚合酶可以在各种高温下具有不同的聚合酶活性。一方面，聚合酶活性包括在多核苷酸的3'端加入脱氧核苷酸。本发明还提供了试剂盒，例如诊断试剂盒和用于进行各种扩增反应的方法，例如聚合酶链反应，转录扩增，连接酶链反应自持序列复制或Qβ复制酶扩增。

10. 发明申请

WO2003002066A2 GLYCOSYLATED KINAMYCINS AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译：甘氨酸的生物素及其制备和使用的方法
公开(公告)号：WO2003002066A2
公开(公告)日：2003-01-09
申请号：PCT/US2002/020719
申请日：2002-06-27
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , PARADKAR, Ashish , VAROGLU, Mustafa , MATHUR, Eric, J.
发明人： SHORT, Jay, M. , PARADKAR, Ashish , VAROGLU, Mustafa , MATHUR, Eric, J.
IPC分类号： A61K
CPC分类号： C07H15/24
摘要： [00138] The invention provides compositions, including pharmaceuticals, comprising glycosylated kinamycins. The compositions and methods of the invention can be used to treat infections, i.e., as antibiotics, and as anti-tumor agents. The compositions of the invention can also act as electrophilic azo-coupling agents in vitro or vivo. The invention also provides enzymes capables of generating kinamycin, nucleic acids that encode them, antibodies that bind to them, and methods for making and using them.
摘要翻译：本发明提供包含糖基化的kinamycins的包含药物的组合物。本发明的组合物和方法可用于治疗感染，即作为抗生素，以及作为抗肿瘤剂。本发明的组合物还可以在体外或体内作为亲电子偶氮偶联剂。本发明还提供产生激霉素的酶能力，编码它们的核酸，与它们结合的抗体，以及制备和使用它们的方法。

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