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1. 发明申请

WO2012158826A3 METHOD AND APPARATUS FOR DETECTION, ANALYSIS, AND COLLECTION OF RARE CELLULAR EVENTS 审中-公开
标题翻译：用于检测，分析和收集稀有细胞活性的方法和装置
公开(公告)号：WO2012158826A3
公开(公告)日：2014-05-08
申请号：PCT/US2012038189
申请日：2012-05-16
申请人： ABBOTT LAB , WU JIONG , JUNNARKAR MAHESH , VACCA GIACOMO
发明人： WU JIONG , JUNNARKAR MAHESH , VACCA GIACOMO
IPC分类号： G01N33/48 , G01N33/50
CPC分类号： G01N15/1459 , G01N15/1434 , G01N21/05 , G01N21/53 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/6482 , G01N2021/6493
摘要： Systems and methods for the detection, analysis, and collection of rare cellular events, wherein rare cellular events are defined by events comprising less than 5% of a total number of cells in a sample. The systems and methods generally include: (1) a flow cell dimensioned so as to permit a flow of a sample through the flow cell at a flow rate greater than 300,000 cells per second; (2) a laser positioned to emit a laser beam directed to the flow cell; (3) one or more deflector components disposed between the laser and the flow cell, wherein the deflector component is configured to affect a position of the laser beam relative to the sample flow; (4) one or more fluorescence emission detectors; and (5) one or more processor configured to detect rare cellular events based on fluorescence emission from cell-binding surface markers introduced into the sample prior to the sample being flowed through the flow cell.
摘要翻译：用于检测，分析和收集罕见细胞事件的系统和方法，其中罕见的细胞事件由包含小于样品中总细胞数量的5％的事件定义。系统和方法通常包括：（1）流动池，其尺寸设计成允许样品以大于每秒300,000个细胞的流速流过流动池; （2）定位成发射指向流动池的激光束的激光器; （3）设置在所述激光器和所述流动池之间的一个或多个偏转器部件，其中所述偏转器部件被配置为影响所述激光束相对于所述样品流的位置; （4）一个或多个荧光发射检测器; 以及（5）一个或多个处理器，被配置为基于在样品流过所述流动池之前引入到样品中的来自细胞结合表面标记物的荧光发射来检测罕见的细胞事件。

2. 发明申请

WO2012151105A2 BASOPHIL ANALYSIS SYSTEM AND METHOD 审中-公开
标题翻译： BASOPHIL分析系统和方法
公开(公告)号：WO2012151105A2
公开(公告)日：2012-11-08
申请号：PCT/US2012035163
申请日：2012-04-26
申请人： ABBOTT LAB , WU JIONG , BUHL MICHAEL R , VACCA GIACOMO
发明人： WU JIONG , BUHL MICHAEL R , VACCA GIACOMO
IPC分类号： C12M1/42 , G01N21/64
CPC分类号： G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2021/6439
摘要： Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
摘要翻译：本文提供了用于分析血液样品的系统和方法，并且更具体地用于进行嗜碱性粒细胞分析。在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; 然后（b）使用光散射和荧光发射的测量来区分嗜碱性粒细胞与其他WBC亚种群。在一个实施方案中，系统和方法包括基于光散射和荧光测量的组合来进行血液样品的嗜碱性粒细胞聚类分析。

3. 发明申请

WO2012151103A3 NUCLEATED RED BLOOD CELL ANALYSIS SYSTEM AND METHOD 审中-公开
标题翻译：核红细胞分析系统及方法
公开(公告)号：WO2012151103A3
公开(公告)日：2014-05-08
申请号：PCT/US2012035161
申请日：2012-04-26
申请人： ABBOTT LAB , WU JIONG , COLEMAN MARILOU , LIN EMILY H , BUHL MICHAEL R , VACCA GIACOMO
发明人： WU JIONG , COLEMAN MARILOU , LIN EMILY H , BUHL MICHAEL R , VACCA GIACOMO
IPC分类号： G01N33/00 , C12M1/34 , C12Q1/06
CPC分类号： G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
摘要： Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
摘要翻译：用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。

4. 发明申请

WO2012151102A3 WHITE BLOOD CELL ANALYSIS SYSTEM AND METHOD 审中-公开
标题翻译：白细胞分析系统和方法
公开(公告)号：WO2012151102A3
公开(公告)日：2014-05-08
申请号：PCT/US2012035158
申请日：2012-04-26
申请人： ABBOTT LAB , WU JIONG , VACCA GIACOMO
发明人： WU JIONG , VACCA GIACOMO
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/008 , G01N2015/1006 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
摘要： Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.
摘要翻译：用于分析血液样品的系统和方法，更具体来说用于进行白细胞（WBC）差异分析。系统和方法通过荧光染色和荧光触发策略筛选WBC。因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。这些系统和方法还可以开发出相对温和的WBC试剂，适用于含有脆弱WBC的样品的测定。在一个实施方案中，系统和方法包括：（a）用发射光谱对应于血液学仪器的激发源的专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发来筛选白细胞的血样; 和（c）使用（1）轴向光损失，（2）中角散射，（3）90°偏振侧散射，（4）90°去极化侧散射的测量，和（5）荧光发射来进行微分分析。

5. 发明申请

WO2011028437A3 METHOD FOR FLAGGING A SAMPLE 审中-公开
标题翻译：标记样本的方法
公开(公告)号：WO2011028437A3
公开(公告)日：2011-10-06
申请号：PCT/US2010046082
申请日：2010-08-20
申请人： ABBOTT LAB , KROCKENBERGER MARTIN , BORDENKIRCHER RICHARD B , GARRETT DIANA G , GLAZIER JOHN G , BEARDEN JAMES C , ROEMER BODO , VACCA GIACOMO
发明人： KROCKENBERGER MARTIN , BORDENKIRCHER RICHARD B , GARRETT DIANA G , GLAZIER JOHN G , BEARDEN JAMES C , ROEMER BODO , VACCA GIACOMO
IPC分类号： G01N15/14 , G01N15/00 , G01N15/10
CPC分类号： G01N33/4915 , G01N15/1429 , G01N15/1459 , G01N33/492 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1486 , G01N2015/1488 , Y10T436/101666 , Y10T436/2575
摘要： A hematology analyzer is provided. In certain embodiments, the hematology analyzer comprises: a) a flow cell; b) a light source for directing light to the flow cell; c) a plurality of detectors for detecting a plurality of optical characteristics of a blood cell passing through the flow cell; and d) a data analysis workstation programmed to: i. enumerate test blood cells passing through the flow cell; and ii. flag a blood sample as containing lysis-resistant red blood cells or fragile white blood cells.
摘要翻译：提供血液分析仪。在某些实施方案中，血液学分析仪包括：a）流动池; b）用于将光引导至流动池的光源; c）多个检测器，用于检测穿过流动池的血细胞的多个光学特性; 和d）数据分析工作站，其编程为：i。列举通过流动池的测试血细胞; 和ii。将血液样本标记为含有抗溶解红血细胞或脆性白血细胞。

6. 发明公开

EP2425241A4 METHOD FOR DISCRIMINATING RED BLOOD CELLS FROM WHITE BLOOD CELLS BY USING FORWARD SCATTERING FROM A LASER IN AN AUTOMATED HEMATOLOGY ANALYZER 审中-公开
标题翻译： DISTINCTION红血细胞白血细胞通过正向溢出由激光在自动血液分析仪法
公开(公告)号：EP2425241A4
公开(公告)日：2015-05-13
申请号：EP10770181
申请日：2010-04-26
申请人： ABBOTT LAB
发明人： KROCKENBERGER MARTIN , WU JIONG , ROEMER BODO , VACCA GIACOMO
IPC分类号： G01N33/48 , G01N15/14
CPC分类号： G01N15/1404 , G01N15/00 , G01N15/1429 , G01N15/1459 , G01N21/53 , G01N33/49 , G01N33/4915 , G01N33/492 , G01N2015/0073 , G01N2015/0076 , G01N2015/008 , G01N2015/0084 , G01N2015/1006 , G01N2015/1486 , G01N2800/22 , Y10T436/101666
摘要： A method for identifying, analyzing, and quantifying the cellular components of whole blood by use of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by use of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

7. 发明公开

EP2705135A4 BASOPHIL ANALYSIS SYSTEM AND METHOD 有权
标题翻译：系统和方法分析Basophiler物质
公开(公告)号：EP2705135A4
公开(公告)日：2015-05-06
申请号：EP12779290
申请日：2012-04-26
申请人： ABBOTT LAB
发明人： WU JIONG , BUHL MICHAEL R , VACCA GIACOMO
IPC分类号： G01N15/14 , G01N15/00
CPC分类号： G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2021/6439
摘要： Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

8. 发明公开

EP2705134A4 WHITE BLOOD CELL ANALYSIS SYSTEM AND METHOD 有权
标题翻译：系统和方法分析血液白细胞
公开(公告)号：EP2705134A4
公开(公告)日：2015-06-17
申请号：EP12779974
申请日：2012-04-26
申请人： ABBOTT LAB
发明人： WU JIONG , VACCA GIACOMO
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： G01N15/1434 , G01N15/1459 , G01N21/6428 , G01N33/49 , G01N2015/008 , G01N2015/1006 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
摘要： Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

9. 发明公开

EP2705136A4 NUCLEATED RED BLOOD CELL ANALYSIS SYSTEM AND METHOD 有权
标题翻译：系统和方法用于分析有核红细胞
公开(公告)号：EP2705136A4
公开(公告)日：2015-05-13
申请号：EP12779936
申请日：2012-04-26
申请人： ABBOTT LAB
发明人： WU JIONG , COLEMAN MARILOU , LIN EMILY H , BUHL MICHAEL R , VACCA GIACOMO
IPC分类号： C12M1/42 , G01N15/10 , G01N15/14 , G01N21/64 , G01N33/50
CPC分类号： G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
摘要： Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

10. 发明专利

ES2746481T3 Determinación del volumen y del contenido de hemoglobina de los glóbulos rojos individuales 未知
公开(公告)号：ES2746481T3
公开(公告)日：2020-03-06
申请号：ES11701332
申请日：2011-01-14
申请人： ABBOTT LAB
发明人： KROCKENBERGER MARTIN , GARRETT DIANA , VACCA GIACOMO
IPC分类号： G01N15/00 , G01N15/14 , G01N33/49
摘要： Un analizador de hematología (10) que comprende: a) una celda de flujo (28); b) una fuente de luz (12) para dirigir la luz a dicha celda de flujo; c) una pluralidad de detectores (32, 34, 36) para detectar la dispersión de luz por las células de una muestra de sangre que se mueve a través de la celda de flujo en una pluralidad de canales de dispersión óptica; d) un transductor colorimétrico para medir la concentración de hemoglobina en un lisado celular; e) un transductor de impedancia para medir los cambios en la impedancia de la muestra de sangre; y f) una estación de trabajo de análisis de datos que comprende una memoria física que contiene instrucciones que, cuando son ejecutadas por la estación de trabajo, hacen que el analizador de hematología: i) calcule el volumen de glóbulos rojos individuales de una muestra que contiene una población de glóbulos rojos usando: datos de dispersión óptica de dispersión de ángulo intermedio (IAS) y de pérdida de luz axial (ALL) de los glóbulos rojos individuales obtenidos por la pluralidad de los canales de dispersión óptica; y mediciones de impedancia de los glóbulos rojos individuales obtenidos usando el transductor de impedancia; o ii) calcule el contenido de hemoglobina de glóbulos rojos individuales de una muestra que contiene una población de glóbulos rojos usando: datos de dispersión óptica de dispersión de ángulo intermedio (IAS) y de dispersión lateral polarizada (PSS) de los glóbulos rojos individuales obtenidos por la pluralidad de los canales de dispersión óptica; mediciones de impedancia de los glóbulos rojos individuales obtenidos usando el transductor de impedancia; y mediciones de la concentración de hemoglobina de los glóbulos rojos individuales obtenidos por el transductor colorimétrico.

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