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    • 1. 发明授权
    • 애멸구 유래 재조합 그로이엘단백질 유전자 클론 및 이를이용한 벼줄무늬 잎마름병 바이러스의 면역포획역전사효소연쇄중합반응 검정방법
    • 来自小褐斑病病人的重组体基因克隆和使用蛋白质蛋白质的米糠病毒免疫测定RT-PCR的检测方法
    • KR100822742B1
    • 2008-04-17
    • KR1020070026577
    • 2007-03-19
    • 대한민국(농촌진흥청장)
    • 이민호이선영조점래엄기백박진우
    • C12N15/31C12N15/09C12N15/10C12Q1/68
    • C12N15/11C12Q1/6888C12Q1/701C12Q2531/113
    • A recombinant protein derived from small brown planthopper is provided to be specifically coupled to thread-shaped rice stripe virus, thereby forecasting precise RSV outbreak, developing a transgenic plant having RSV resistance and being applied as various tools for detecting the RSV. A recombinant protein described in SEQ ID : NO. 2 is obtained by expressing a recombinant protein in a gene clone(deposition no. KACC-95060P) described in SEQ ID : NO. 1, which is constructed by inserting a GroEL gene isolated from small brown planthopper into an expression vector, and then purifying the protein using His-taq Ni-NTA agarose column and is characterized in that it is specifically coupled to thread-shaped RSV. A method for detecting an RSV gene comprises the steps of: (a) coating a reaction container which is PolySorp treated with the GroEL recombinant protein derived from the small brown planthopper or an antibody thereof with a coating solution; (b) treating the reaction solution with a detecting sample through coupling reaction with the with the RSV to immuno-capturing RSV particles; and (c) after isolating genes from the immuno-captured RSV particles, subjecting the isolated genes to RT-PCR to detect genes of the RSV.
    • 提供了一种来源于小型褐飞虱的重组蛋白,特异性地与丝状水稻条纹病毒偶联,从而预测了精确的RSV疫情,开发了具有RSV抗性的转基因植物,并被用作检测RSV的各种工具。 SEQ ID NO: 2是通过在SEQ ID NO:1中描述的基因克隆(沉积编号KACC-95060P)中表达重组蛋白获得的。 1,其通过将从小褐飞虱分离的GroEL基因插入表达载体中构建,然后使用His-taq Ni-NTA琼脂糖柱纯化蛋白质,其特征在于其特异性结合于螺纹状RSV。 检测RSV基因的方法包括以下步骤:(a)用来自小褐飞虱或其抗体的GroEL重组蛋白用涂布溶液涂覆PolySorp处理的反应容器; (b)用检测样品通过与RSV的偶联反应处理反应溶液以免疫捕获RSV颗粒; 和(c)从免疫捕获的RSV颗粒中分离出基因后,将分离的基因进行RT-PCR检测RSV的基因。