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1. 发明专利

JP2011182674A Keratinase and method for producing the same 有权
标题翻译：制造方法及其制造方法
公开(公告)号：JP2011182674A
公开(公告)日：2011-09-22
申请号：JP2010049279
申请日：2010-03-05
申请人： Aohisa Hirooka , Kyoto Ichi , Akira Sasaki , Akihiko Sukeno , Chihiro Takasaka , Naohiro Tomari , Jun Wada , Kunihiko Watabe , Yoshihiro Yamamoto , 京都市 , 晃佐々木 , 彰彦助野 , 潤和田 , 佳宏山本 , 青央廣岡 , 直宏泊 , 邦彦渡部 , 千尋高阪
发明人： WATABE KUNIHIKO , SASAKI AKIRA , SUKENO AKIHIKO , YAMAMOTO YOSHIHIRO , HIROOKA AOHISA , TAKASAKA CHIHIRO , TOMARI NAOHIRO , WADA JUN
IPC分类号： C12N9/42 , A61H33/00 , A61K8/66 , A61Q5/00 , A61Q19/10
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently producing a keratinase having new properties from a safe fungus strain and a keratinase obtained by the method. SOLUTION: The method for producing a keratinase includes inoculating a fungus of the genus Aspergillus into potatoes, beans or grains, performing a solid culture, immersing the obtained culture products in water at 25-60°C and extracting a keratinase. The keratinase obtained by the method is provided. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供一种从安全菌株和通过该方法获得的角蛋白酶有效生产具有新特性的角蛋白酶的方法。解决方案：生产角蛋白酶的方法包括将曲霉属的真菌接种到马铃薯，豆或谷粒中，进行固体培养，将获得的培养物浸入25-60℃的水中并提取角蛋白酶。提供通过该方法获得的角蛋白酶。版权所有（C）2011，JPO＆INPIT

2. 发明专利

JP2009298141A Document holder accessory 审中-公开
标题翻译：文件夹附件
公开(公告)号：JP2009298141A
公开(公告)日：2009-12-24
申请号：JP2009107216
申请日：2009-04-26
申请人： Yoshihiro Yamamoto , ▲吉▼宏山本
发明人： YAMAMOTO YOSHIHIRO
IPC分类号： B42F1/02 , B42F9/00 , B42F11/00
摘要： PROBLEM TO BE SOLVED: To provide a document holder accessory which can hold a document exactly irrespective of the thickness of the document. SOLUTION: The back piece 3 and the first pressing piece 5 of the document holder accessory 1 are constituted of material having elasticity, and the document 10 of the thickness below a standard gap GS is sandwiched between the tip part 62 of a second pressing piece 6 disposed at the sandwiching position and a document placing piece 2. The document 10 of the thickness exceeding the standard gap GS is sandwiched between the document pressing unit 52 of the first pressing piece 5 and the document placing piece 2 in the state where the second pressing piece 6 is disposed at the opening position. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种文件夹附件，其可以与文件的厚度无关地保持文件。解决方案：文件夹附件1的后片3和第一按压片5由具有弹性的材料构成，厚度低于标准间隙GS的文件10被夹在第二个设置在夹持位置的按压片6和文件放置件2.超过标准间隙GS的厚度的文件10被夹在第一按压片5的原稿按压单元52和文件放置片2之间，第二按压片6设置在打开位置。版权所有（C）2010，JPO＆INPIT

3. 发明专利

JP2009229379A Method and system for measuring atp-ase activity using isfet 有权
标题翻译：使用ISFET测量ATP-ASE活性的方法和系统
公开(公告)号：JP2009229379A
公开(公告)日：2009-10-08
申请号：JP2008077625
申请日：2008-03-25
申请人： Bio X:Kk , Aohisa Hirooka , Kyoto Ichi , Yoshihiro Yamamoto , 京都市 , 佳宏山本 , 青央廣岡 , 株式会社バイオエックス
发明人： TANI TOSHIO , YAMAMOTO YOSHIHIRO , HIROOKA AOHISA
IPC分类号： G01N27/414 , C12M1/34 , C12Q1/42 , G01N27/416
摘要： PROBLEM TO BE SOLVED: To provide a method capable of measuring a phosphoric acid caused by ATP activity reaction and changes in its concentration with high sensitivity, and a system using the same. SOLUTION: The method and the system uses an ion sensitive field effect transistor (ISFET) sensor using tantalum pentoxide for a sensing section and having a configuration to detect ion concentration by detecting a change amount in potential level of a channel based on changes in the surface potential of the sensing section, the sensor having the sensing section sensitive to ions formed on a gate insulation film of a field effect transistor (FET), for measuring the ATP-ase activity of protein having the ATP-ase activity. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种能够测量由ATP活性反应引起的磷酸及其浓度变化高灵敏度的方法及其使用方法。解决方案：该方法和系统使用使用五氧化二钽作为感测部分的离子敏感场效应晶体管（ISFET）传感器，并且具有通过基于变化检测通道的电位变化量来检测离子浓度的配置在感测部分的表面电位中，传感器具有对形成在场效应晶体管（FET）的栅极绝缘膜上的离子敏感的感测部分，用于测量具有ATP-活性的蛋白质的ATP-活性。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2009222514A Measuring method of concentration of pyrophosphoric acid and measuring method for nucleic acid synthesizing reaction using the same 审中-公开
标题翻译：磷酸浓度的测定方法和使用该反应的核酸合成反应的测定方法
公开(公告)号：JP2009222514A
公开(公告)日：2009-10-01
申请号：JP2008066364
申请日：2008-03-14
申请人： Bio X:Kk , Kyoto Ichi , Kyoto Institute Of Technology , Toyobo Co Ltd , Wakenyaku Kk , Yoshihiro Yamamoto , 京都市 , 和研薬株式会社 , 国立大学法人京都工芸繊維大学 , 佳宏山本 , 東洋紡績株式会社 , 株式会社バイオエックス
发明人： MURAKAMI AKIRA , YAMAMOTO YOSHIHIRO , TANI TOSHIO , YAMATANI MASAKAZU , NISHIYA YOSHIAKI
IPC分类号： G01N27/414 , C12N15/09 , C12Q1/68 , G01N27/416
摘要： PROBLEM TO BE SOLVED: To provide a novel quantifying method of pyrophosphoric acid in a sample, a novel measuring method of nucleic acid synthesizing reaction using it and a system for these methods. SOLUTION: In a system for detecting the concentration of pyrophosphoric acid by using an ion responsive field effect transistor (ISFET) sensor, a sensor provided with a tantalum pentoxide layer is designated as the ISFET sensor. Especially, in the measuring method of the concentration of pyrophosphoric acid in the sample, the cumulative type ISFET sensor, which is constituted so as to measure the charge quantity accumulated after potential is reset as a potential change, corresponding to the depth of a potential well changed, corresponding to the concentration of the ions acting on a sensing part and the pump-out number of times from the potential well is used. The measuring method of nucleic acid synthesizing reaction is constituted so as to measure the change in the concentration of pyrophosphoric acid which is produced as the byproduct of the polymerase reaction to target nucleic acid, by using the measuring method of the concentration of pyrophosphoric acid. At least the ISFET sensor and the system for measuring the nucleic acid synthesizing reaction in the sample having polymerase activity are constituted. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：为了提供样品中焦磷酸的新型定量方法，使用它的核酸合成反应的新的测量方法和这些方法的系统。解决方案：在通过使用离子响应场效应晶体管（ISFET）传感器检测焦磷酸浓度的系统中，设置有五氧化二钽层的传感器被指定为ISFET传感器。特别是，在测定样品中焦磷酸浓度的测定方法中，累积型ISFET传感器构成为测量电位复位后积蓄的电荷量作为电位变化，对应于势阱的深度对应于作用在感测部分上的离子的浓度而变化，并且使用来自势阱的抽出次数。构成核酸合成反应的测定方法，通过使用焦磷酸的浓度的测定方法测定作为聚合酶反应的副产物产生的焦磷酸浓度的变化。至少构成ISFET传感器和用于测量具有聚合酶活性的样品中的核酸合成反应的系统。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2010274253A Cleaning apparatus of portable duct 有权
标题翻译：便携式清洁器的清洁装置
公开(公告)号：JP2010274253A
公开(公告)日：2010-12-09
申请号：JP2009146967
申请日：2009-05-30
申请人： Matsubara Yoko Kk , Odagiri Katsuhiko , Yoshihiro Yamamoto , 小田桐勝彦 , 良廣山本 , 松原熔工株式会社
发明人： YAMAMOTO YOSHIHIRO
IPC分类号： B08B9/032 , B08B9/027
摘要： PROBLEM TO BE SOLVED: To provide an apparatus that automates cleaning operation of a portable duct. SOLUTION: A large pulley of a pushing-up mechanism is rotated and at the same time advanced to the outer periphery of a duct, a small pulley is abutted on a resin sheet part of the duct and pushed into the inner peripheral side. In accordance with the rotation of the large pulley, the small pulley is made to push up the resin sheet upward. Thus, the resin sheet of the duct is automatically pushed up in order. Meantime, cleaning nozzles are arranged on the center side of a duct placing base and in a position opposing the outer periphery of the duct, of the duct placing base. The inner periphery and the outer periphery of the duct can be cleaned by jetting cleaning water from these cleaning nozzles. In addition, since the resin sheet of the duct is pushed up in order by the pushing-up mechanism, automatic cleaning is possible over the entire length of the duct. Further, the duct placing base is moved up and down by an oscillating cylinder, consequently the cleaning nozzles move vertically with respect to the duct and repeated cleaning is automatically performed. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种使便携式导管的清洁操作自动化的装置。解决方案：上推机构的大的皮带轮旋转并同时前进到管道的外周，小皮带轮抵靠在管道的树脂片部分上并被推入内周侧。根据大型滑轮的旋转，使小滑轮向上推动树脂片。因此，管道的树脂片被依次自动上推。同时，清洁喷嘴布置在管道放置基座的中心侧并且与管道布置基座的管道的外周相对的位置。可以通过从这些清洁喷嘴喷射清洁水来清洁管道的内周和外周。此外，由于通过上推机构依次向上推动导管的树脂片，所以在导管的整个长度上可进行自动清洗。此外，管道放置基座通过振荡筒上下移动，因此清洁喷嘴相对于管道垂直移动，并且自动执行重复清洁。版权所有（C）2011，JPO＆INPIT

6. 发明专利

JP2009083108A Document folder 审中-公开
标题翻译：文件文件夹
公开(公告)号：JP2009083108A
公开(公告)日：2009-04-23
申请号：JP2007251833
申请日：2007-09-27
申请人： Yoshihiro Yamamoto , ▲吉▼宏山本
发明人： YAMAMOTO YOSHIHIRO
IPC分类号： B42F9/00
摘要： PROBLEM TO BE SOLVED: To provide a document folder, with a cover, which can bind documents without perforating and does not mar its overall appearance while retaining a significant nipping force, and further, facilitates an attaching/detaching operation of documents.
SOLUTION: This document folder 1 includes a cover body 2 and a clip 3. In addition, a nipping piece 34 of the clip 3 is urged in the direction in which the nipping piece 34 rotates to a release position from a nipping position through a closest approach position (the R2 direction of Fig. centering around a hinge part 36) by the elastic restoring force of the hinge part 36. Besides, an almost rectangular opening 23 is arranged in the cover body 2, and the nipping piece 34 comes into contact with the document 10 directly through the opening 23, when the nipping piece 34 is located at the nipping position. When attempting to draw out the document 10, a strong force works to bite the document 10 and, in turn, the document 10 is nipped by an extremely strong force. In the meantime, an operating piece 35 covers the opening 23 when the nipping piece 34 is located at the nipping position.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供具有盖的文件夹，其可以在没有穿孔的情况下装订文件，并且在保持显着的夹持力的同时不损害其整体外观，并且还有助于文件的附接/拆卸操作。解决方案：该文件夹1包括盖体2和夹子3.此外，夹子3的夹持件34沿着夹持件34从夹持位置旋转到释放位置的方向被推动通过铰链部36的弹性恢复力，通过最靠近的接近位置（图中心的R2方向）。此外，盖体2中布置有几乎矩形的开口23，并且夹持件34 当夹紧片34位于夹持位置时，直接通过开口23与原稿10接触。当试图拉出文件10时，强力工作以咬住文件10，并且反过来，文件10被非常强的力夹住。同时，当夹持片34位于夹持位置时，操作片35覆盖开口23。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2008216004A Biosensor 审中-公开
标题翻译：生物传感器
公开(公告)号：JP2008216004A
公开(公告)日：2008-09-18
申请号：JP2007052956
申请日：2007-03-02
申请人： Bio X:Kk , Kyoto Ichi , Toyobo Co Ltd , Yoshihiro Yamamoto , 京都市 , 佳宏山本 , 東洋紡績株式会社 , 株式会社バイオエックス
发明人： NISHIYA YOSHIAKI , TANI TOSHIO , YAMAMOTO YOSHIHIRO
IPC分类号： G01N27/414
摘要： PROBLEM TO BE SOLVED: To provide a new quantitative determination method of cholesterol ester in a sample, and a system for it. SOLUTION: The measuring method of cholesterol ester includes a process of making enzyme react with the cholesterol ester in the sample, a process of detecting the concentration variation of ions generated by the reaction using an accumulation type ISFET sensor, and a process of quantitatively determining the cholesterol ester in the sample based on the detected ion concentration variation. The system for the measuring method is also provided. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供样品中胆固醇酯的新的定量测定方法及其系统。解决方案：胆固醇酯的测定方法包括使酶与样品中的胆固醇酯反应的过程，使用累积型ISFET传感器检测由反应产生的离子的浓度变化的过程，以及基于检测到的离子浓度变化，定量测定样品中的胆固醇酯。还提供了测量方法的系统。版权所有（C）2008，JPO＆INPIT

8. 发明专利

JP2008209143A Measuring method of creatinine and system for it 审中-公开
标题翻译： CREATININE及其系统的测量方法
公开(公告)号：JP2008209143A
公开(公告)日：2008-09-11
申请号：JP2007044133
申请日：2007-02-23
申请人： Bio X:Kk , Kyoto Ichi , Toyobo Co Ltd , Yoshihiro Yamamoto , 京都市 , 佳宏山本 , 東洋紡績株式会社 , 株式会社バイオエックス
发明人： NISHIYA YOSHIAKI , TANI TOSHIO , YAMAMOTO YOSHIHIRO
IPC分类号： G01N27/414
摘要： PROBLEM TO BE SOLVED: To provide a new quantitatively measuring method of creatinine in a sample, and to provide a system for the same. SOLUTION: The measuring method of creatinine includes; a process of reacting creatinine in a specimen with an enzyme reactive with creatinine; a process of detecting change in ion concentration generated by the reaction using an accumulator type ISFET sensor; and a process of quantitatively measuring creatinine in the specimen based on the detected change in ion concentration. A system for the same is also disclosed. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供样品中肌酸酐的新的定量测定方法，并提供一种用于其的系统。
解决方案：肌酐的测定方法包括：使样品中的肌酸酐与肌酸酐反应的酶反应的过程; 使用蓄电池型ISFET传感器检测由反应产生的离子浓度的变化的过程; 以及基于检测出的离子浓度的变化来定量测定样品中的肌酐的过程。还公开了一种用于其的系统。版权所有（C）2008，JPO＆INPIT

9. 发明专利

JP2007218781A Isoelectric point electrophoretic apparatus 有权
标题翻译： ISOELECTRIC POINT ELECTROPHORETIC APPARATUS
公开(公告)号：JP2007218781A
公开(公告)日：2007-08-30
申请号：JP2006040833
申请日：2006-02-17
申请人： Makoto Endo , Aohisa Hirooka , Kyoto Ichi , Toyobo Co Ltd , Yoshihiro Yamamoto , 京都市 , 佳宏山本 , 青央廣岡 , 東洋紡績株式会社 , 真遠藤
发明人： YAMAMOTO YOSHIHIRO , HIROOKA AOHISA , ENDO MAKOTO , NISHIYA YOSHIAKI
IPC分类号： G01N27/447
摘要： PROBLEM TO BE SOLVED: To provide an isoelectric point electrophoretic apparatus, capable of recovering target protein in a solution state with high efficiency using a simple operation. SOLUTION: The isoelectric point electrophoretic apparatus has at least one electrophoretic module constituted of a connection block having a sample chamber for housing a sample solution and a pair of gel-filled columns, having different pHs arranged so that the end surfaces of them come into contact with the sample solution in the sample chamber through the connection block, the anode tank and the cathode tanks, both of which are respectively connected to a pair of the gel filled columns becoming both ends of the electrophoretic module and house an electrolytic liquid and the anode and cathode, respectively arranged on the anode and cathode tanks. By supplying a current to the anode and the cathode, the target protein in the sample solution, having an isoelectric point between the pH of one gel filled column and the pH of the other gel-filled column. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种等电点电泳装置，其能够使用简单的操作以高效率在溶液状态下回收目标蛋白质。等电点电泳装置具有至少一个电泳模块，该电泳模块由具有用于容纳样品溶液的样品室的连接块和一对具有不同pH值的凝胶填充柱组成，使得其端面通过连接块，阳极罐和阴极罐与样品室中的样品溶液接触，两者分别连接到一对凝胶填充柱，成为电泳模块的两端并容纳电解液阳极和阴极分别布置在阳极和阴极罐上。通过向阳极和阴极提供电流，样品溶液中的靶蛋白，其具有在一个凝胶填充柱的pH之间的等电点和另一个凝胶填充的柱的pH。版权所有（C）2007，JPO＆INPIT

10. 发明专利

JP2007083701A Paper holder auxiliary apparatus 有权
标题翻译：纸架辅助设备
公开(公告)号：JP2007083701A
公开(公告)日：2007-04-05
申请号：JP2006035369
申请日：2006-02-13
申请人： Yoshihiro Yamamoto , ▲吉▼宏山本
发明人： YAMAMOTO YOSHIHIRO , SHIBATA HIROSHI , KATANODA KOSEI
IPC分类号： B42F9/00 , B42F1/02
摘要： PROBLEM TO BE SOLVED: To provide a paper holder auxiliary apparatus which easily holds papers with a paper holder apparatus without opening an inserting hole on papers, also hold them for every item, easily carries out taking out operation of the held papers, and further reads the paper in a state of the papers being held with the paper holder.
SOLUTION: A detent part 2 having an inserting hole and a pinching part 3 are provided. The pinching part 3 is equipped with: a paper receptive part 40; a pinching main body piece 4; a pinching piece 5; a coupling part 7; and a pinching piece operating part 6, and pinches the paper received in the paper receptive part 40 with the pinching main body piece 4 and the pinching piece 5. After pinching the paper, the paper is fixed in the paper holder with the detent part 2.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：为了提供一种纸张辅助装置，其容易地用纸张保持装置保持纸张，而不在纸上打开插入孔，也可以容纳每个物品，容易地执行持有的纸张的取出操作，并进一步读取纸张与纸架保持在一起的状态。
解决方案：设有具有插入孔和夹持部3的棘爪部2。夹持部3配备有：纸容纳部40; 夹持主体件4; 夹紧片5; 联接部7; 和夹持件操作部6，并且用夹持主体部件4和夹持件5夹持容纳在接收部分40中的纸张。在夹持纸张之后，纸张被固定在纸夹中，并具有制动部件2 。版权所有（C）2007，JPO＆INPIT

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