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1. 发明授权

US11714031B2 Method for visualizing neurons 有权
公开(公告)号：US11714031B2
公开(公告)日：2023-08-01
申请号：US16881932
申请日：2020-05-22
申请人： Yeu-Kuang Hwu
发明人： Yeu-Kuang Hwu , Shin-Tai Chen , Shun-Min Yang
IPC分类号： G01N1/30 , G01N1/36
CPC分类号： G01N1/30 , G01N1/36 , G01N2001/302 , G01N2001/305 , G01N2001/364
摘要： A method for visualizing neurons is provided. The method comprises a staining step and a visualizing step. The staining step comprises following steps: placing a neural tissue sample in an acrolein solution in the dark for fixation; placing the fixed neural tissue sample in a Golgi-Cox solution in the dark; replacing the Golgi-Cox solution; incubating the neural tissue sample placed in the replaced Golgi-Cox solution at a range of 36° C. to 38° C.; gradiently dehydrating the neural tissue sample; and embedding the dehydrated neural tissue sample with Petropoxy 154 resin. The visualizing step comprises: performing data acquisition and image reconstruction on the neural tissue sample using X-ray microscopy.

2. 发明授权

US11371924B2 Method for imaging compound contained by lipid vesicle in water and examining method for the same 有权
公开(公告)号：US11371924B2
公开(公告)日：2022-06-28
申请号：US16729052
申请日：2019-12-27
申请人： Yeu-Kuang Hwu
发明人： Yeu-Kuang Hwu , Yu-Fang Hu , Shun-Min Yang , Chi-Feng Huang
IPC分类号： G06K9/00 , G01N15/02 , A61K9/127 , G06T7/00 , A61K31/704 , H01S3/09
摘要： The present disclosure provides a method for imaging a compound contained by a lipid vesicle in water. The method comprises the following steps of: (a) providing an aqueous sample comprising the lipid vesicle which contains the compound, wherein the aqueous sample further comprises ammonium sulphate ((NH4)2SO4); (b) illuminating the aqueous sample with an X-ray free-electron laser (X-FEL); (c) with an image sensor, collecting a plurality of coherent diffraction image patterns of the aqueous sample being illuminated; and (d) reconstructing the coherent diffraction image patterns with a computer such that an image of the lipid vesicle containing the compound is acquired. A method for examining a quality of a chemical drug contained by a liposome in water is also provided.

3. 发明授权

US10114016B2 Particles and manufacturing methods thereof 有权
公开(公告)号：US10114016B2
公开(公告)日：2018-10-30
申请号：US12545822
申请日：2009-08-22
申请人： Yeu-Kuang Hwu , Chang-Hai Wang , Chi-Jen Liu , Cheng-Liang Wang , Chi-Hsiung Chen , Chung-Shi Yang , Hong-Ming Lin , Jung-Ho Je , Giorgio Margartondo
发明人： Yeu-Kuang Hwu , Chang-Hai Wang , Chi-Jen Liu , Cheng-Liang Wang , Chi-Hsiung Chen , Chung-Shi Yang , Hong-Ming Lin , Jung-Ho Je , Giorgio Margartondo
IPC分类号： B22F9/24 , G01N33/543 , B22F1/00 , B22F9/02 , B82Y30/00 , C01B13/36 , C01B33/12 , C01G49/08 , C09C1/00 , C09C1/04 , C09C1/30 , C09C1/36 , C09C1/62 , C09C3/04
摘要： Particles and manufacturing methods thereof are provided. The manufacturing method of the particle includes providing a precursor solution containing a precursor dissolved in a solution, and irradiating the precursor solution with a high energy and high flux radiation beam to convert the precursor to nano-particles. Particles with desired dispersion, shape, and size are manufactured without adding a stabilizer or surfactant to the precursor solution.

4. 发明申请

US20120052573A1 ONE-POT SYNTHESIS OF LINEAR-LIKE POLYETHYLENIMINE FOR INTRACELLULAR IMAGING AND NUCLEIC ACID DELIVERY 审中-公开
标题翻译：用于细胞成像和核酸递送的线性聚乙烯亚胺的一步合成
公开(公告)号：US20120052573A1
公开(公告)日：2012-03-01
申请号：US12868939
申请日：2010-08-26
申请人： Shu-Yi LIN , Fong-Sian LIN , Meng-Kai CHEN , Yu-Chen JAO , Lin-Ren TSAI , Hong-Yi LIN , Chung-Shi YANG , Yeu-Kuang HWU
发明人： Shu-Yi LIN , Fong-Sian LIN , Meng-Kai CHEN , Yu-Chen JAO , Lin-Ren TSAI , Hong-Yi LIN , Chung-Shi YANG , Yeu-Kuang HWU
IPC分类号： C12N5/09 , A61K31/7088 , C08G73/00
CPC分类号： C08G73/0206 , C08G73/0213 , C08L79/02
摘要： A method of synthesizing polyethyleneimine with a substantially linear backbone is disclosed. The method comprises exposing ethylenediamine dissolved in a solution to electromagnetic radiation for a sufficient time to polymerize the ethylenediamine and thereby resulting in formation of polyethylenimine with a substantially linear backbone in the solution. A method of synthesizing a homopolymer with a substantially linear backbone is also disclosed. In addition, a composition comprising polyethylenimine synthesized from the aforementioned method is disclosed, in which the polyethylenimine comprises a backbone conformation that is substantially linear and has a distribution of molecular weights (MW) ranging from 1 kDa to 200 kDa; and the polyethyleneimine has no cytotoxicity at a concentration of 12 μg/ml.
摘要翻译：公开了一种合成具有基本线性主链的聚亚乙基亚胺的方法。该方法包括将溶解在溶液中的乙二胺暴露于电磁辐射足够的时间以使乙二胺聚合，从而导致在溶液中形成具有基本线性主链的聚乙烯亚胺。还公开了一种合成具有基本线性主链的均聚物的方法。另外，公开了包含由上述方法合成的聚乙烯亚胺的组合物，其中聚乙烯亚胺包括基本上线性且具有范围从1kDa至200kDa的分子量（MW）分布的主链构型; 并且聚乙烯亚胺在12μg/ ml的浓度下没有细胞毒性。

5. 发明申请

US20090202650A1 METHODS OF TREATING CANCERS 有权
标题翻译：治疗癌症的方法
公开(公告)号：US20090202650A1
公开(公告)日：2009-08-13
申请号：US12028026
申请日：2008-02-08
申请人： Yeu-Kuang Hwu , Tsung-Yeh Yang , Chi-Jen Liu , Chang-Hai Wang
发明人： Yeu-Kuang Hwu , Tsung-Yeh Yang , Chi-Jen Liu , Chang-Hai Wang
IPC分类号： A61K9/14 , A61P35/00 , A61N5/10 , A61K33/24 , A61K33/30 , A61K33/38 , A61M36/02
CPC分类号： A61K33/24 , A61K9/0009 , A61K33/30 , A61K41/00 , A61N5/10 , A61N2005/1098 , A61K2300/00
摘要： A method of treating cancer. The method includes introducing an effective amount of an oxidative catalyzing agent including titanium oxide, zinc oxide, zirconium oxide, tungsten oxide or tin oxide into a biological entity, and irradiating the biological entity with a ray. The oxidative catalyzing agent produces hydroxyl or hydrogen peroxide radicals after irradiation with the ray thereon.
摘要翻译：一种治疗癌症的方法。该方法包括将有效量的包含氧化钛，氧化锌，氧化锆，氧化钨或氧化锡的氧化催化剂引入生物体中，并用射线照射生物体。氧化催化剂在用其上的射线照射后产生羟基或过氧化氢自由基。

6. 发明申请

US20170087640A1 PHOTOLUMINESCENT GOLD NANOPARTICLES AND MANUFACTURING METHOD THEREOF 审中-公开
公开(公告)号：US20170087640A1
公开(公告)日：2017-03-30
申请号：US15085449
申请日：2016-03-30
申请人： Yeu-Kuang HWU
发明人： Yeu-Kuang HWU , Sheng-Feng LAI , Edwin Bin-Leong ONG
IPC分类号： B22F9/24 , C22B3/00
CPC分类号： B22F9/24 , B22F1/0018 , B22F1/0062 , B22F2999/00 , B82Y30/00 , B82Y40/00 , C22B11/04 , B22F2202/11
摘要： Photoluminescent gold nanoparticles and the manufacturing method thereof are disclosed. The method for manufacturing photoluminescent gold nanoparticles includes the steps of: preparing a solution containing chloroauric acid and alkanethiolate, wherein the alkanethiolate-to-Au molar ratio is at least 1; and irradiating the solution with ionizing radiation to form gold nanoparticles, wherein the surfaces of the gold nanoparticles are coated with the alkanethiolate to form thiolate-coated gold nanoparticles with gold cores.

7. 发明申请

US20120225924A1 SINGLE-MONOMER DERIVED LINEAR-LIKE COPOLYMER COMPRISING POLYETHYLENIMINE AND POLY(ETHYLENE GLYCOL) FOR NUCLEIC ACID DELIVERY 有权
标题翻译：包含聚乙烯胺和聚（乙二醇）用于核酸递送的单一单体衍生的线型共聚物
公开(公告)号：US20120225924A1
公开(公告)日：2012-09-06
申请号：US13041023
申请日：2011-03-04
申请人： Shu-Yi Lin , Chung-Shi Yang , Yeu-Kuang Hwu
发明人： Shu-Yi Lin , Chung-Shi Yang , Yeu-Kuang Hwu
IPC分类号： A61K31/7105 , A61P35/04 , C08G73/04
CPC分类号： A61K31/713 , C08G73/024
摘要： A method of synthesizing a random copolymer of polyethyleneimine and polyethylene glycol, comprising exposing ethanolamine in a solution to electromagnetic radiation for a sufficient time to polymerize the ethanolamine (OHCH2CH2NH2) and thereby resulting in formation of the randome copolymer comprising polyethyleneimine and poly(ethylene glycol), wherein the polyethyleneimine comprises ethyleneimine (—CH2CH2NH—) unit and the polyethylene glycol comprises ethylene glycol (—CH2CH2O—) unit, and the polyethylenimine of the random copolymer has a substantially linear backbone.
摘要翻译：一种合成聚乙烯亚胺和聚乙二醇的无规共聚物的方法，包括将溶液中的乙醇胺暴露于电磁辐射足够的时间以聚合乙醇胺（OHCH 2 CH 2 NH 2），从而形成包含聚乙烯亚胺和聚（乙二醇）其中聚乙烯亚胺包含乙烯亚胺（-CH 2 CH 2 NH-）单元，聚乙二醇包括乙二醇（-CH 2 CH 2 O-）单元，无规共聚物的聚乙烯亚胺具有基本线性的主链。

8. 发明授权

US07991454B2 Imaging of biological structures 有权
标题翻译：生物结构成像
公开(公告)号：US07991454B2
公开(公告)日：2011-08-02
申请号：US10435717
申请日：2003-05-08
申请人： Yeu-Kuang Hwu , Giorgio Margaritondo , Jung Ho Je
发明人： Yeu-Kuang Hwu , Giorgio Margaritondo , Jung Ho Je
IPC分类号： A61B6/00
CPC分类号： A61B6/482 , A61B6/4092 , A61B6/481 , A61B6/484 , A61B6/504 , A61B6/508
摘要： Images of blood vessels of a body are obtained by injecting a refraction enhancement contrast agent into the blood vessels to increase a difference in refractive indices of the blood vessels and surrounding material. The blood vessels are irradiated with a penetrating radiation, and an image of the blood vessels is generated based on detected radiation. The image has visible edge enhancement features indicating boundaries of the blood vessels.
摘要翻译：通过将折射增强造影剂注射到血管中以增加血管和周围材料的折射率差异来获得身体血管的图像。用穿透性辐射照射血管，并且基于检测到的辐射产生血管的图像。该图像具有指示血管边界的可见边缘增强特征。

9. 发明授权

US06526121B1 Apparatus and method for imaging an object with real-time response 有权
标题翻译：具有实时响应对物体成像的装置和方法
公开(公告)号：US06526121B1
公开(公告)日：2003-02-25
申请号：US09537628
申请日：2000-03-29
申请人： Yeu-Kuang Hwu , Jung Ho Je , Giorgio Margaritondo
发明人： Yeu-Kuang Hwu , Jung Ho Je , Giorgio Margaritondo
IPC分类号： G21K100
CPC分类号： A61B6/484 , A61B6/00 , A61B6/4092 , G01N23/04
摘要： A new radiography method which utilizes contrast enhancement mechanisms with highly collimated X-ray beams without optics to achieve high imaging resolution and improve the time resolution is disclosed. This invention includes irradiating the object with an unmonochromatized beam, specifically highly collimated synchrotron radiation, and detecting an unmonochromatized beam image after the unmonochromatized beam has passed through the object. With compact design, a system for imaging an object with very high resolution, X-ray radiography with a wide range of X-ray sources, such as synchrotron radiation, without any sophisticated X-ray optics is also disclosed. This invention may achieve real-time images with micrometer resolution.
摘要翻译：公开了一种新的射线照相方法，其利用对比度增强机制与高度准直的X射线束无光学实现高成像分辨率并提高时间分辨率。本发明包括用非单色化光束特别是高度准直的同步加速器辐射照射物体，以及在非单色化光束已经通过物体之后检测非单色化光束图像。通过紧凑的设计，还公开了一种用于对具有非常高分辨率的物体进行成像的系统，具有各种X射线源的X射线摄影，例如同步加速器辐射，而没有任何复杂的X射线光学器件。本发明可以实现千分尺分辨率的实时图像。

10. 发明申请

US20200284705A1 METHOD FOR VISUALIZING NEURONS 审中-公开
公开(公告)号：US20200284705A1
公开(公告)日：2020-09-10
申请号：US16881932
申请日：2020-05-22
申请人： YEU-KUANG HWU
发明人： YEU-KUANG HWU , SHIN-TAI CHEN , SHUN-MIN YANG
IPC分类号： G01N1/30 , G01N1/36
摘要： A method for visualizing neurons is provided. The method comprises a staining step and a visualizing step. The staining step comprises following steps: placing a neural tissue sample in an acrolein solution in the dark for fixation; placing the fixed neural tissue sample in a Golgi-Cox solution in the dark; replacing the Golgi-Cox solution; incubating the neural tissue sample placed in the replaced Golgi-Cox solution at a range of 36° C. to 38° C.; gradiently dehydrating the neural tissue sample; and embedding the dehydrated neural tissue sample with Petropoxy 154 resin. The visualizing step comprises: performing data acquisition and image reconstruction on the neural tissue sample using X-ray microscopy.

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