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    • 6. 发明公开
    • COMPARTMENTALIZED TISSUE CULTURE BAG
    • 数字化的组织培养袋
    • EP0817834A1
    • 1998-01-14
    • EP96911468.0
    • 1996-03-28
    • Wolf, Martin L.Wilson, John R.
    • Wolf, Martin L.Wilson, John R.
    • C12M1C12M3C12N5
    • C12M29/04C12M23/14C12M23/24C12M23/26
    • A compartmentalized cell culture bag (10) comprising a cell culture compartment (40) defined by a lower gas permeable film (120) and an upper membrane (20) selectively permeable to compounds of selected sizes. Culture medium (50) resides between the upper membrane (20) and the lower gas permeable film (120). A basal medium compartment (30) is located above the upper membrane (20) and allows basal medium (60) to reside upon the upper membrane (20). Each compartment contains an access port (70, 90). A gas film support (130) below and in partial contact with the gas permeable film (120) holds the gas permeable film (120) in a substantially horizontal position so that suspension or adherent cells can distribute across the surface of the gas permeable film (120).
    • 包括由较低气体可渗透膜(120)限定的细胞培养隔室(40)和选择性渗透所选尺寸化合物的上部隔膜(20)的隔室化细胞培养袋(10)。 培养基(50)位于上部膜(20)和下部气体可渗透膜(120)之间。 基础培养基隔室(30)位于上部隔膜(20)上方并且允许基础培养基(60)驻留在上部隔膜(20)上。 每个隔间都包含一个存取端口(70,90)。 气体膜支撑件(130)在透气膜(120)的下方并与其部分接触,将透气膜(120)保持在基本上水平的位置,使得悬浮或粘附的细胞可以分布在透气膜的表面上 120)。