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    • 9. 发明专利
    • Nucleus export reporter system
    • AU2481002A
    • 2002-05-15
    • AU2481002
    • 2001-10-30
    • WAGNER RALF
    • WAGNER RALFGRAF MARCUS
    • C12N15/85C12Q1/68C12Q1/6897C12N15/86
    • Detecting RNA export from the nucleus of a eukaryotic cell, comprising preparing a nucleic acid (I) that encodes a reporter protein (RP) and produces a transcript that can be exported from the nucleus, introducing (I) into the nucleus so that, in the nucleus, it is operably linked to a transcription control sequence, transcription from (I), and determining if the transcript is exported, is new. Detecting RNA export from the nucleus of a eukaryotic cell, comprising preparing a nucleic acid (I) that encodes a reporter protein (RP) and produces a transcript that can be exported from the nucleus, introducing (I) into the nucleus so that, in the nucleus, it is operably linked to a transcription control sequence, transcription from (I), and determining if the transcript is exported, is new. Export of the transcript depends on presence of a cis-active RNA export signal, operably linked to (I), a trans-activating factor, and optionally a functional 5'-splice donor, in absence of 3'-splice acceptor. The RP-encoding segment of the transcript can not undergo splicing. Independent claims are also included for the following: (1) DNA sequence (II) that contains RP-encoding sequence, modified from the wild type so that, unlike the wild type, RNA export occurs, depending on a linked cis-acting RNA export element, and optionally a functional 5'-splice donor in absence of 3'-splice acceptor; (2) eukaryotic cell containing (II) in transcribable form; (3) reagent kit comprising (II) or cell transfected by it, and/or the cells of (2); (4) detecting viral infection by detecting activity of a viral nuclear export factor; (5) DNA sequence (III) encoding a fluorescent protein (FP), that is at least partly codon-optimized for a retrovirus, specifically human immune deficiency virus (HIV)-1; (6) DNA sequence (IV) comprising a sequence encoding FP between functional 5'-splice donor and 3'-splice acceptor sites; and (7) detecting RNA export from nuclei of eukaryotic cells, using an RP that can be detected without lysis or fixing of cells.