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1. 发明申请

WO2019125804A1 NUCLEASES AND METHODS FOR MAKING AND USING THEM 审中-公开
公开(公告)号：WO2019125804A1
公开(公告)日：2019-06-27
申请号：PCT/US2018/064718
申请日：2018-12-10
申请人： BASF SE , TAN, Xuqiu
发明人： TAN, Xuqiu , BARRETT, Kenneth , DENNIS, Jared , REN, Dongmei , DODGE, Corey , ZHANG, Bin
IPC分类号： C12N9/22 , C12N15/10
CPC分类号： C12Y301/30002 , C12N9/22
摘要： Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis ("GSSM") method or the tailored multi-site combinatorial assembly ("TMCA") method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

2. 发明申请

WO2005086900A3 PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：磷脂，核酸编码及其制备与使用方法
公开(公告)号：WO2005086900A3
公开(公告)日：2009-04-02
申请号：PCT/US2005007908
申请日：2005-03-08
申请人： DIVERSA CORP , GRAMATIKOVA SVETLANA , HAZLEWOOD GEOFF , LAM DAVID , BARTON NELSON R , STURGIS BLAKE G , ROBERTSON DAN E , LI JINCAI , KREPS JOEL A , FIELDING RODERICK , BROWN ROBERT C , VASAVADA AMIT , TAN XUQIU , BADILLO ADRIAN , VAN HOEK WILHELMUS P , JANSSEN GISELLE , ISAAC CHARLES , BURK MARK J
发明人： GRAMATIKOVA SVETLANA , HAZLEWOOD GEOFF , LAM DAVID , BARTON NELSON R , STURGIS BLAKE G , ROBERTSON DAN E , LI JINCAI , KREPS JOEL A , FIELDING RODERICK , BROWN ROBERT C , VASAVADA AMIT , TAN XUQIU , BADILLO ADRIAN , VAN HOEK WILHELMUS P , JANSSEN GISELLE , ISAAC CHARLES , BURK MARK J
IPC分类号： C12N9/20 , C07H21/04 , C12N1/20 , C12N9/16 , C12N15/00
CPC分类号： C12N15/8242 , C07K2319/00 , C12N9/16 , C12N9/18 , C12N9/20 , C12N15/8247 , C12P7/6418 , C12P7/6481
摘要： The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.
摘要翻译：本发明提供具有磷脂酶活性的新型多肽，包括例如磷脂酶A，B，C和D活性，patatin活性，磷脂酸磷酸酶（PAP））和/或脂质酰基水解酶（LAH）活性，编码它们的核酸和抗体绑定到他们。还提供了工业方法，例如油脱胶，以及包含使用这些磷脂酶的产品。

3. 发明申请

WO2020106599A1 A RECOMBINANT YEAST CELL 审中-公开
公开(公告)号：WO2020106599A1
公开(公告)日：2020-05-28
申请号：PCT/US2019/061929
申请日：2019-11-18
申请人： BASF SE , TAN, Xuqiu
发明人： TAN, Xuqiu , ZHONG, Jingping , SCRANTON, Melissa Ann , LI, Daphne
IPC分类号： C12N1/16 , C12N15/81
摘要： The present invention relates to a recombinant yeast cell for high yield protein expression. The invention further relates to cell culture involving the recombinant yeast cell, a method for preparing protein involving culturing the recombinant yeast cell and a use of the recombinant yeast cell.

4. 发明申请

WO2005086900A2 PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：磷脂，核酸编码及其制备与使用方法
公开(公告)号：WO2005086900A2
公开(公告)日：2005-09-22
申请号：PCT/US2005/007908
申请日：2005-03-08
申请人： DIVERSA CORPORATION , GRAMATIKOVA, Svetlana , HAZLEWOOD, Geoff , LAM, David , BARTON, Nelson, R. , STURGIS, Blake, G. , ROBERTSON, Dan, E. , LI, Jincai , KREPS, Joel, A. , FIELDING, Roderick , BROWN, Robert, C. , VASAVADA, Amit , TAN, Xuqiu , BADILLO, Adrian , VAN HOEK, Wilhelmus, P. , JANSSEN, Giselle , ISAAC, Charles , BURK, Mark, J.
发明人： GRAMATIKOVA, Svetlana , HAZLEWOOD, Geoff , LAM, David , BARTON, Nelson, R. , STURGIS, Blake, G. , ROBERTSON, Dan, E. , LI, Jincai , KREPS, Joel, A. , FIELDING, Roderick , BROWN, Robert, C. , VASAVADA, Amit , TAN, Xuqiu , BADILLO, Adrian , VAN HOEK, Wilhelmus, P. , JANSSEN, Giselle , ISAAC, Charles , BURK, Mark, J.
IPC分类号： C12N9/16
CPC分类号： C12N15/8242 , C07K2319/00 , C12N9/16 , C12N9/18 , C12N9/20 , C12N15/8247 , C12P7/6418 , C12P7/6481
摘要： The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.
摘要翻译：本发明提供具有磷脂酶活性的新型多肽，包括例如磷脂酶A，B，C和D活性，patatin活性，磷脂酸磷酸酶（PAP））和/或脂质酰基水解酶（LAH）活性，编码它们的核酸和抗体绑定到他们。还提供了工业方法，例如油脱胶，以及包含使用这些磷脂酶的产品。

5. 发明申请

WO2020106600A1 LEADER SEQUENCE FOR YEAST 审中-公开
公开(公告)号：WO2020106600A1
公开(公告)日：2020-05-28
申请号：PCT/US2019/061930
申请日：2019-11-18
申请人： BASF SE , TAN, Xuqiu
发明人： TAN, Xuqiu , ZHONG, Jingping , SCRANTON, Melissa, Ann
IPC分类号： C11D3/386 , C12N9/20 , C12R1/84
摘要： The present invention relates to a leader peptide which promotes the secretion of recombinant proteins and a nucleic acid sequence encoding the leader peptide as well as expression cassettes, vectors and host cells comprising this leader sequence. Also disclosed is a method for producing a protein using this leader peptide.

6. 发明申请

WO2019236451A1 PROMOTER FOR YEAST 审中-公开
公开(公告)号：WO2019236451A1
公开(公告)日：2019-12-12
申请号：PCT/US2019/035140
申请日：2019-06-03
申请人： BASF SE , TAN, Xuqiu
发明人： TAN, Xuqiu , CAI, Ruorong , ZHONG, Jingping , SCRANTON, Melissa Ann , SPEER, Michael
IPC分类号： C12N15/31 , C12N15/81 , C07K14/39 , C12N1/19 , C12N1/16 , C12P21/00
摘要： A promoter operably linked to a gene encoding a protein is disclosed. The promoter drives expression of the protein in a yeast cell in the absence of methanol. Also disclosed are vectors, host cells and expression systems that include the promoter, as well as methods of using the promoter to express proteins in yeast.

7. 发明申请

WO2009018449A1 TAILORED MULTI-SITE COMBINATORIAL ASSEMBLY 审中-公开
标题翻译：定制多地点组合装配
公开(公告)号：WO2009018449A1
公开(公告)日：2009-02-05
申请号：PCT/US2008/071771
申请日：2008-07-31
申请人： VERENIUM CORPORATION , TAN, Xuqiu
发明人： TAN, Xuqiu
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N15/102 , C12N15/1024
摘要： The present invention provides a novel method of producing a plurality of modified polynucleotides having different combinations of various mutations at multiple sites by a tailored multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the primers are not overlapping, and wherein each of the primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers. The method can be performed without employing a ligation step prior to transforming the extended modified polynucleotides into a cell. The plurality of extended modified polynucleotides can be treated with an enzyme for destroying the template polynucleotide prior to transforming in to the cell.
摘要翻译：本发明提供了一种通过定制的多位点组合组合产生多个具有多个位点的不同突变组合的多个修饰的多核苷酸的新方法，包括向双链模板多核苷酸中添加至少两个或至少三个引物单反应混合物，其中引物不重叠，并且其中每个引物包含与其它引物不同的至少一个突变，其中至少一个引物是可以与模板的负链退火的至少一个引物，并且至少一个引物是可以与模板的正链退火的反向引物，并且使反应混合物进行聚合酶延伸反应，以从至少三种引物产生多个延伸的修饰的多核苷酸。在将延伸的修饰的多核苷酸转化成细胞之前，可以不使用连接步骤进行该方法。多个延伸修饰的多核苷酸可以在用于在转化到细胞之前破坏模板多核苷酸的酶处理。

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